Abstract
The present study was conducted to evaluate some of the components of antioxidant defense system and oxidative damage in 20 male patients of alcoholic liver disease (ALD). The results were compared with 20 healthy male smokers and 20 healthy male non-smokers volunteers. Patients were subjected to detailed clinical examination and laboratory investigations. Blood samples were collected for estimating reduced glutathione (GSH), total thiols (T-SH) malondialdehyde (MDA), transaminases (AST, ALT), glutathione-S-transferease (GST) and gammaglutamyl transferase (GGT). Serum aspartate amino transferase (AST)/alanine amino transferase (ALT) ratio was significantly (p<0.01) reduced in ALD patients as compared to the controls. However, the core of utility of MDA and GST was found to be significantly (p<0.01) increased in ALD patients compared to controls. There was a significant negative correlation of MDA with both GSH and TSH. Plasma GGT levels were significantly (p<0.01) increased in alcoholics and the enzyme showed a significant positive correlation with MDA. These results give enough evidence of increased oxidative stress and compromised antioxidant defense system in patients with ALD.
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Liber, C.S. (2000) Alcohol and liver: metabolism of alcohol and its role in hepatic and extrahepatic diseases. J. Med. 67(1), 84–94.
Sherlock, S. (1995). Alcohol and the liver. In: Sherlock S. Diseases of the liver and biliary system. 6th ed. Blackwell publications London, pp 385–403.
Halliwell B. (1989). Free radical, reactive oxygen species and human disease. Br. J. Exp. Pathol. 70, 737–57.
Hirnwich, H.E., Nahum, L.H., Pakieten, N., Fazekas, J.F., Du Bots, H. (1982). Effects of alcohol on metabolism. Am. J. Physiol. 101, 57–68.
Frienhel, N., Aky, R.A., Singer, D.L., Cohen, A.K. (1985). Alcohol hypoglycemia IV. Current concepts of its pathogenesis. Diabetes 4, 350–361.
Halliwell, B., Gutlridge, J.M.C. (1990). The antioxidant of human extra-cellular fluids. Arch. Biochem. Biophys 280 (1), 1–8.
Datla, K., Sinha, S., Chattopadhyay, P. (2000). Reactive oxygen species in health and disease. Natl. Med. J. Ind. 13 (6), 305–311.
Beutler, E., Duron, O., Kelly, B.M. (1963). Improved method for the determination of blood glutathione. J. Lab. Clin. Med 61, 882–888.
Ellman, G.L. (1959). Tissue sulfhydryl groups. Arch. Biochem. Biophy., 82, 70–77.
Beuge, J.A., Aust, S.D. (1978). The thiobarbituric acid assay. Meth. Enzymol. 52, 306–307.
Habig, W.H., Pabst, M.J., Jakoby, W.B. (1974). Glutathione-S-transferase, the first enzymatic step in mercapaturic acid formation. J. Biochem. 249, 7130–7139.
Szass, M. (1976). Committee of enzymes of the Scandinavian society for clinical chemistry and clinical physiology: Recommended method for the determination of gamma-glutamyl transferase in blood. Scand. J. Clin. Lab. Invest. 36, 119–125.
Jose, M.M., Javer, F.P., Florence, C., Susana, C., Antonia, C. (1999). S-adenosyl methionine in alcoholic liver cirrhosis: a randomized, placebo controlled, double-blind multi-centre clinical trial. J. Hepatol. 30, 1081–1089.
Lieber, C.S. (1984). Metabolism and metabolic effects of alcohol. Med. Clin. North Am. 68 (1), 3–31.
Morimotto, M., Hagbjork, A.I., Nanji, A.A. (1993). Role of cytochrome P450 2EI in alcoholic liver disease pathogenesis. Alcohol 10, 459–64.
Nordmann, R., Ribiere, C., Rouach, H. (1992). Implication of free radical mechanisms in ethanol included cellular injury. Free Radical Biol. Med. 12, 219–240.
Peters, T.J., Ward, R.J. (1998). Tole of acetaldehyde in the pathogenesis of alcohol liver disease. Mol. Aspects Med. 10, 179–190.
Pyorr, W.A., Stone, K. (1993). Oxidants in cigarette smoke: Radicals, hydrogen peroxides, peroxynitrate and peroxynitrite. Ann, N.Y. Acad. Sci. 686, 12–28.
Cohen, J.A., Kaplan, M.M. (1979). The SGOT/SGPT ratio an indication of alcoholic liver disease. Dig. Dis. Sci. 24, 835–838.
Lieber, C.S. (1998). Biochemical and molecular basis of alcohol induced injury to liver and other tissues. N. Engl. J. Med. 319, 1639–1650.
Tatsuo, S., Sato, C., Maromo, F. (1992). Significance of plasma glutathione determination in patients with alcoholic and non-alcoholic liver disease. J. Gastroenterol. Hepatol. 7, 7–11.
Loguercio, C., Blanco, Dr., Girdamo, D., Disalco, D., Nardi, -G. (1999). Ethanol consumption, amino acid and glutathione blood levels in patients with and without chronic liver disease. Alcoholism Clin. Expt. Res. 24, 1780–84.
Checa, J.C.F., Hirano, T., Tsukamoto, H., Kaplowitz, N., (1993). Mitochondrial glutathione deflection in alcoholic liver disease. Alcohol 10, 469–475.
Poli, G. (1993). Liver damage due to free radicals. Br. Med. Bull. 49(3), 604–620.
Penn, R., Worthington, D.J. (1983). Is serum gamma glutamyl transferase a misleading test. Br. Med. J. 286, 531–535.
Whitefield, J.B., Moss, D.W., Nelae, G. (1973). Changes in plasma gamma glutamyl transpeptidase activity associated with alteration in drug metabolism in man. Br. Med. J. 1, 316–318.
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Gupta, S., Pandey, R., Katyal, R. et al. Lipid peroxide levels and antioxidant status in alcoholic liver disease. Indian J Clin Biochem 20, 67–71 (2005). https://doi.org/10.1007/BF02893045
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DOI: https://doi.org/10.1007/BF02893045