Abstract
Lymph node status is still the single most important prognostic factor in breast cancer and surgery remains the only reliable means of providing this information. This study evaluates using a highly specific radiolabeled monoclonal antibody to provide equivalent information.
The optimum labeling conditions for radiolabeling a monoclonal antibody against the gene product of the protooncogene c-erbB-2 with Tc99m were established. This immunoconjugate was next evaluated in a mouse model system and averaged 20% localization of the total injected dose per gram of tumor at 24h.
Ten patients have had this immunoconjugate, with planar and tomographic reconstructed images being obtained at 24 h. The resulting images were compared to histopathological examination of the surgical specimens. Three patients acted as normal controls, two patients were selected on the basis of inappropriate sampling of adjacent ductal carcinomain situ, three patients demonstrated only moderate antigen expression, and two patients demonstrated excellent tumor localization in both breast primary and regional node metastases.
The high specificity of this antibody, ease of labeling, and excellent localization performance with a good antigen target encourage the development of this system as a method of localization and a potential means of antibody-guided therapy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Weinstein, J. N., Covell, D. G., Keenan, A. M., Holton, O. D., Steller, M. A., Sieber, S. M., et al. (1984)Hybridoma 3, 66.
Vaickus, L. and Foon, K. A. (1991)Cancer Invest. 9, 195–209.
Bradwell, A. R., Fairweather, D. S., Dykes, P. W., Keeling, A., Vaughan, A., and Taylor, J. (1985)Immunol. Today 6, 163–170.
Schlom, J. (1989)J. Am. Med. Assoc. 261, 744–746.
Styles, J. M., Harrison, S., Gusterson, B. A., and Dean, C. J. (1990)Int. J. Cancer 45, 320–324.
Slamon, D. J., Clark, G. M., Wong, S. G., Lewin, W. J., Ullrich, A., and McGuire, W. L. (1987)Science 235, 177–182.
Gusterson, B. A., Machin, L. G., Gullick, W. J., Gibbs, N. M., Powles, T. J., Price, P., et al. (1988)Int. J. Cancer 42, 842–845.
Perren, T. J. (1992)Br. J. Cancer 63, 328–332.
Dean, C. J., Styles, J., Valeri, M., Modjtahedi, H., Bakir, A., Babich, J. W., et al. (1992) Growth factor receptors as targets for antibody therapy, inMutant Oncogenes: Targets for Therapy? (Epenetos, A. A. and Lemoine, N., eds.), 27–34.
Bakir, M. A., Eccles, S. A., Babich, J. W., Aftab, N., Styles, J. M., Dean, C. J., et al. (1992)J. Nucl. Med. 33, 2154–2160.
Fernando, I. N., Allan, S. M., Sandle, J., Sacks, N. P. M., and Trott, P. A. (1993)Cytopathology 4, 219–224.
Schwarz, A. and Steinstraesser, A. (1987)J. Nucl. Med. 28, A695-A721.
Mather, S. J. and Ellison, D. (1990)J. Nucl. Med. 31, 692–697.
Wright, C., Nicholson, S., Angus, S., Sainsbury, J. R. C., Farndon, J., Cairns, J., et al. (1992)Br. J. Cancer 65, 118–121.
Gusterson, B. A., et al. For the International (Ludwig) Breast Cancer Study Group (1992)J. Clin. Oncol. 10, 1049–1056.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Allan, S.M., Dean, C.J., Eccles, S. et al. Clinical radioimmunolocalization with a rat monoclonal antibody directed against c-erbB-2. Cell Biophysics 24, 93–98 (1994). https://doi.org/10.1007/BF02789219
Issue Date:
DOI: https://doi.org/10.1007/BF02789219