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Skin equivalent produced with human collagen

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Summary

Several studies have recently been conducted on cultured skin equivalent (SE), prepared using human keratinocytes seeded on various types of dermal equivalents (DE). We previously showed the advantages of our anchorage method in preventing the severe surface reduction of DE due to fibroblast contractile properties in vitro. A new anchored human SE was established in our laboratory in order to obtain a bioengineered tissue that would possess the appropriate histological and biological properties. In order to compare the effects of different collagen origins on the evolution of SE in vitro, human keratinocytes were seeded on three types of anchored DE. A comparative study was carried out between bovine SE (bSE), human SE (hSE), and human skin equivalent containing additional dermal matrix components (hSE +). Immunohistological analysis showed that hSE and hSE+ presented good structural organization, including the deposition of several basement membrane constituents. Higher amounts of transglutaminase, ceramides, and keratin 1 were detected in the epidermal layers of all SE when cultured at the air-liquid interface. However, a 92 kDa gelatinase activity was higher in bovine skin equivalent (bSE) compared to hSE cultures. The use of human collagens comparatively to bovine collagen as SE matricial component delayed the degradation of the dermal layer in culture.

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Authors and Affiliations

  1. Laboratoire de Recherche des Grands Brûlés/LOEX, Saint-Sacrement Hospital, G1S 4L8, Quebec, QC, Canada

    François A. Auger, Carlos A. López Valle, Rina Guignard, Nathalie Tremblay, Bernard Noel, Francine Goulet & Lucie Germain

  2. Department of Surgery and Department of Microbiology, Laval University Faculty of Medicine, G1K 7P4, Sainte-Foy, QC, Canada

    François A. Auger, Carlos A. López Valle, Rina Guignard, Nathalie Tremblay, Bernard Noel, Francine Goulet & Lucie Germain

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  1. François A. Auger
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  2. Carlos A. López Valle
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  3. Rina Guignard
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  4. Nathalie Tremblay
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  5. Bernard Noel
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  6. Francine Goulet
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  7. Lucie Germain
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Auger, F.A., López Valle, C.A., Guignard, R. et al. Skin equivalent produced with human collagen. In Vitro Cell Dev Biol - Animal 31, 432–439 (1995). https://doi.org/10.1007/BF02634255

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  • DOI: https://doi.org/10.1007/BF02634255

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