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A method for the continuous cultivation of mammary epithelium

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Summary

Established cell lines of mammary epithelium have been obtained from mice, rats, and hamsters. Maintenance and replication of the epithelium in serial subcultures were dependent on their periodic treatment with collagenase. Because collagenase is not cytotoxic and has maximum efficiency at neutral pH in isotonic saline solutions containing calcium and magnesium, this enzyme can be introduced directly into the culture medium; cells have been maintained for 3 days in such a medium with serous enrichment at no detriment to them. Up to 10% concentrations of serum have not interfered with enzymatic activity.

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Authors and Affiliations

  1. Department of Cytological Biophysics, Institute for Medical Research, 08103, Camden, New Jersey

    Etienne Y. Lasfargues & Dan H. Moore

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  1. Etienne Y. Lasfargues
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  2. Dan H. Moore
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Additional information

Supported by United States Public Health Service Grant CA-08515 and CA-08740 from the National Cancer Institute. General Research Support Grant FR-5582 from the National Institutes of Health, and Grant-in-Aid Contract M-43 from the State of New Jersey.

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Lasfargues, E.Y., Moore, D.H. A method for the continuous cultivation of mammary epithelium. In Vitro 7, 21–25 (1971). https://doi.org/10.1007/BF02619001

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  • DOI: https://doi.org/10.1007/BF02619001

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