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Effect of interleukin 1 beta on osteoblastic clone MC3T3-E1 cells

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Summary

The effect of recombinant interleukin 1 Beta (IL-1(β)) was investigated on osteoblastic cell line MC3T3-E1 cloned from mouse calvaria. IL-1(β) stimulated cell proliferation which increased cell number and caused dose-related stimulation of DNA synthesis, with a maximal effect at a concentration of 12.5 U/ml; suppressed alkaline phosphatase activity and collagen synthesis maximally at 0.5 and 62.5 U/ml, respectively; and increased the amount of free [3H] hydroxyproline in the cultures, but the amount was quite low. Prostaglandin E2 synthesis was also stimulated dose dependently by the presence of IL-1(β), with a maximal increase at 2.5 U/ml, at which concentration the prostaglandin E2 level in the medium was 1.61±0.10 ng/ml. The increased prostaglandin E2 synthesis did not affect either the IL-1(β)-mediated change in DNA or collagen synthesis or alkaline phosphatase activity. These results extend the possibility that IL-1(β) is to act as a regulator of bone formation.

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Authors and Affiliations

  1. Department of Oral Anatomy, Josai Dental University, Sakado, Saitama, Japan

    Eiko Ikeda, Yoshiyuki Hakeda & Masayoshi Kumegawa

  2. Department of Biochemistry, Tokushima University, School of Medicine, Kuramoto-cho, Tokushima, Japan

    Masami Kusaka & Shozo Yamamoto

  3. Laboratory of Food Chemistry, Department of Agricultural Chemistry, Simane University, Matsue, Japan

    Kazushige Yokota

Authors
  1. Eiko Ikeda
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  2. Masami Kusaka
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  3. Yoshiyuki Hakeda
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  4. Kazushige Yokota
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  5. Masayoshi Kumegawa
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  6. Shozo Yamamoto
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Ikeda, E., Kusaka, M., Hakeda, Y. et al. Effect of interleukin 1 beta on osteoblastic clone MC3T3-E1 cells. Calcif Tissue Int 43, 162–166 (1988). https://doi.org/10.1007/BF02571314

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  • DOI: https://doi.org/10.1007/BF02571314

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