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Murine osteoblastlike cells and the osteogenic cell MC3T3-E1 release a macrophage colony-stimulating activity in culture

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Summary

The osteoclast may be of hematopoietic lineage and as such its development could be regulated by colony-stimulating factors. Since there is much interest as to whether osteoblasts influence bone resorption, we examined whether bone cells produce colony-stimulating activity. Both cells isolated from neonatal calvaria and the osteogenic cell MC3T3-E1 were found to constitutively release a colony-stimulating activity possessing characteristics of a macrophage colony-stimulating factor, as determined by basic biochemical purification and by identity of colonies induced in cultures of bone marrow cells. Release could be increased by the presence of the bone-resorbing agents lipopolysaccharide and 1,25 dihydroxyvitamin D3. We conclude that the osteoblast may contribute to both the processes of osteoclast formation and of hematopoiesis through the secretion of colony-stimulating activity into the adjacent bone marrow.

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Authors and Affiliations

  1. Department of Pathophysiology, University of Berne, Murtenstrasse 35, CH-3010, Berne, Switzerland

    P. R. Elford, R. Felix, M. Cecchini, U. Trechsel & H. Fleisch

Authors
  1. P. R. Elford
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  2. R. Felix
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  3. M. Cecchini
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  4. U. Trechsel
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  5. H. Fleisch
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Elford, P.R., Felix, R., Cecchini, M. et al. Murine osteoblastlike cells and the osteogenic cell MC3T3-E1 release a macrophage colony-stimulating activity in culture. Calcif Tissue Int 41, 151–156 (1987). https://doi.org/10.1007/BF02563795

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  • DOI: https://doi.org/10.1007/BF02563795

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