Summary
It has been hypothesized that phagocytosis of a layer of mineralized material which is sometimes deposited along the root surface following trauma is an important factor in the initiation of the resorption of teeth [1]. To test this hypothesis, freezing of the mouse incisor periodontium was used as a model where deposition of mineral was prevented by the systemic administration of 1-hydroxyethylidene-1,1-bisphosphonate (HEBP, 10 mg P/kg b.w.) for time periods varying from 2 to 31 days. The animals were killed either 24 hours or 1 month after the last injection and their mandibles were processed for light microscopy. In the HEBP-treated animals, killed 24 hours after the last injection, no mineralized material had been deposited along the root surface, and very little resorption was seen. Large amounts of bone matrix were deposited in the intraperiodontal space (often causing localized ankylosis). In HEBP-treated animals killed 1 month after the last injection, again no layer of mineralized material along the root surface was seen, but now root resorption had become manifest, occupying up to 40% of the cement surface. This occurred irrespective of the duration of HEBP administration. In the saline-treated animals, extensive resorption of the incisor was noted (up to 90% of its surface). It is concluded that the layer of mineralized material deposited after the periodontium is frozen does not play a decisive part in the onset of root resorption. HEBP treatment slows down root resorption but does not prevent it.
Similar content being viewed by others
References
Wesselink PR, Beertsen W, Everts V (1986) Resorption of the mouse incisor after the application of cold to the periodontal attachment apparatus. Calcif Tissue Int 39:11–21
Andreasen JO (1981) Traumatic injuries of the teeth. Munksgaard, Copenhagen, pp 211–218
Everts V, Beertsen W (1981) Root resorption induced by application of liquid nitrogen to the periodontium. J Dent Res (Special Issue) A60:338
Francis MD, Russell RGG, Fleisch H (1969) Diphosphonates inhibit formation of calcium phosphate crystals in vitro and pathological calcification in vivo. Science 165:1264–1266
Beertsen W, Niehof A, Everts V (1985) Effects of 1-hydroxyethylidene 1,1-bisphosphonate (HEBP) on the formation of dentin and the periodontal attachment apparatus in the mouse. Am J Anat 174:83–103
Russell RGG, Mühlbauer RC, Bisaz S, Williams DA, Fleisch H (1970) The influence of pyrophosphate, condensed phosphates, phosphonates and other phosphate compounds on the dissolution of hydroxyapatite in vitro and on bone resorption induced by parathyroid hormone in tissue culture and in thyroparathyroidectomized rats. Calcif Tissue Int 6:183–196
Reynolds JJ, Minkin C, Morgan DB, Fleisch H (1972) The effect of two diphosphonates on the resorption of mouse calvaria in vitro. Calcif Tissue Res 10:302–313
Boonekamp PM, Van der Wee-Pals LJA, Van Wijk-van Lennep NML, Wil Thesing C, Bijvoet OLM (1986) Two modes of action of bisphosphonates on osteoclastic resorption of mineralized matrix. Bone and Mineral 1:27–39
Chambers TJ, Thomson BM, Fuller K (1984) Effect of substrate composition on bone resorption by rabbit osteoclasts. J Cell Science 70:61–71
Melcher AH (1970) Repair of wounds in the periodontium. Arch Oral Biol 15:1183–1196
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wesselink, P.R., Beertsen, W. Theinnfluenceoof 1-hydroxyethylidene-1,1-bisphosphonate (HEBP) on dental root resorption in the mouse. Calcif Tissue Int 45, 104–110 (1989). https://doi.org/10.1007/BF02561409
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02561409