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Effects of cyclosporine a on chick osteoclastsIn vitro

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Summary

We have studied the effects of cyclosporine A (CsA) on basal and bovine parathyroid hormone (1–34) (bPTH)-stimulated bone resorption by osteoclasts in 24-hour cultures of chick long bone cells. At a high concentration (10 μg/ml), CsA had a cytotoxic effect on both osteoclasts and mononuclear cells in the culture. At 1 μg/ml, CsA inhibited basal and bPTH-stimulated bone resorption but was not cytotoxic over 24 hours. We also studied the binding of bPTH to the osteoblastic cell line, Saos-2, and chick long bone cells in suspension culture. CsA inhibited bPTH binding in Saos-2 in a dose-dependent manner; inhibition of binding was also observed in chick bone cells. The effects of CsA on osteoclast viability and resorptive function may be due to a direct effect on the osteoclasts and/or to an interaction with the nonosteoclastic cell population in the culture.

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Authors and Affiliations

  1. Helen Hayes Hospital, New York State Dept. of Health, Regional Bone Center, Route 9 W, 10993, West Haverstraw, New York, USA

    Majeedul H. Chowdhury, Victor Shen & David W. Dempster

  2. Department of Pathology, Columbia University, 10032, New York, New York, USA

    David W. Dempster

Authors
  1. Majeedul H. Chowdhury
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  2. Victor Shen
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  3. David W. Dempster
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Chowdhury, M.H., Shen, V. & Dempster, D.W. Effects of cyclosporine a on chick osteoclastsIn vitro . Calcif Tissue Int 49, 275–279 (1991). https://doi.org/10.1007/BF02556217

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  • DOI: https://doi.org/10.1007/BF02556217

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