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T-cell subsets and T-cell receptor Vβ utilization by Igh-1-congenic mice in herpetic retinal necrosis

  • Vitreo-Retinal
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Abstract

• Background: After unilateral anterior chamber (AC) inoculation with herpes simplex virus type 1 (HSV-1), C.B-17 and BALB/c congenic mice, which differ only in a limited region around the Igh-1 locus on chromosome 12, show a striking difference in susceptibility to development of encephalitis and contralateral necrotizing chorioretinitis. • Methods: After AC inoculation with HSV-1 (KOS), C.B-17 and BALB/c mice were followed up for the clinical signs of encephalitis and chorioretinitis. At different time points following inoculation, lymphocytes isolated from the spleen were triple-stained with antibodies directed against CD4 or CD8, IL-2R, and various V β T-cell receptor (TCR) subsets, and were analyzed by flow cytometry. • Results: These Igh-l-disparate congenic mice showed differences in the time course of splenic V β T-cell receptor (TCR) usage in both CD4+, IL-2R+ and CD8+, IL-2R+ T cells. By day 1 post infection (p.i.), C.B-17 mice showed an increase of V β 8 and V β 9 TCR by both CD4+, IL-2R+ and CD8+, IL-2R+ splenic T cells. Susceptible BALB/c mice delayed the increase of splenic V β 8 and V β 9 TCR by CD4+, IL-2R+ T cells, which was noted by day 4 p.i. Furthermore, in BALB/c mice the usage of V β 9 by CD8+ cells was increased by day 6 p.i. • Conclusions: Our findings indicate that early preferential splenic usage of a restricted repertoire of TCR occurs after ocular inoculation with HSV-1 in resistant C.B-17 mice. Such preferential TCR usage by activated T cells may prevent viral replication in the brain and contralateral eye and may be linked to protection from development of encephalitis and destructive herpes-mediated ocular inflammation.

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Authors and Affiliations

  1. Hilles Immunology Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts, USA

    Alejandro Berra, Arnd Heiligenhaus & C. Stephen Foster

  2. Department of Pathology, University of Buenos Aires, Buenos Aires, Argentina

    Alejandro Berra

  3. Department of Ophthalmology, University of Essen, Hufelandstrasse 55, D-45122, Essen, Germany

    Arnd Heiligenhaus

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  1. Alejandro Berra
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  2. Arnd Heiligenhaus
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Berra, A., Heiligenhaus, A. & Foster, C.S. T-cell subsets and T-cell receptor Vβ utilization by Igh-1-congenic mice in herpetic retinal necrosis. Graefe's Arch Clin Exp Ophthalmol 234 (Suppl 1), S83–S88 (1996). https://doi.org/10.1007/BF02343053

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  • DOI: https://doi.org/10.1007/BF02343053

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