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Anti-dsDNA and Sm autoantibodies in systemic lupus erythematosus

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Summary

During the present study the coincidence of anti-dsDNA and Sm antibodies was detected in 16 percent of 51 consecutive SLE patients. These antibodies were detected by the standard indirect immunofluorescence and Ouchterlony tests. All patients with anti-dsDNA and Sm antibodies showed disease activity, including renal, CNS and pulmonary disease. We excluded a cross reactivity of these antibodies by ELISA, using competitive experiments with dsDNA and Sm antigens. The results support the presence of multiple autoantibody production during SLE activity, and suggest that different mechanisms may underlie the induction and regulation of both autoantibodies.

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References

  1. Isenberg, D.A., Shoenfeld, Y., Schwartz, R.S. Multiple serological reactions and their relationship to clinical activity in systemic lupus erythematosus. Arthritis Rheum 1984, 27, 132–138.

    Article  CAS  PubMed  Google Scholar 

  2. Smeek, R.T., Lucassen, V.A.M., Swaak, T.J.G. Is cardiolipin activity a cross reaction of anti-DNA or a separate entity. Arthritis Rheum 1987, 30, 607–617.

    Article  Google Scholar 

  3. Fisher, D.E., Reeves, W.H., Wisniewolski, R., Lahita, R.G., Chiorazzi, N. Temporal shifts from Sm to ribonucleoprotein reactivity in systemic lupus erythematosus. Arthritis Rheum 1985, 28, 1348–1355.

    Article  CAS  PubMed  Google Scholar 

  4. Rauch, J.A.V., Tannenbaum, H., Senecal, J.L., Janoff, A.S., Cullis, P.R., Frojmovic, M.M. Polyfunctional properties of hybridoma lupus anticoagulant antibodies. J Rheumatol 1987, 14 (suppl 13), 132–137.

    Google Scholar 

  5. Terborg, E.J., Horst, G., Hummel, E., Jaarsma, D., Limburg, P.C., Kallenberg, C.G.M. Sequential development of antibodies to specific Sm polypeptides in a patient with systemic lupus erythematosus: Evidence for independent regulation of anti-doublestranded DNA and anti-Sm antibody production. Arthritis Rheum 1988, 31, 1563–1567.

    Article  CAS  Google Scholar 

  6. Tan, E.M., Cohen, A.S., Fries, J.F., Masi, A.T., McShane, D.J., Rothfield, N., Schaller, J.G., Talal, N., Winchester, R.J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982, 25, 1271–1277.

    Article  CAS  PubMed  Google Scholar 

  7. Miller, M.H., Urowitz, M.B., Gladman, D.D., Killinger, D.W. Systemic lupus erythematosus in males. Medicine 1983, 62, 327–334.

    Article  CAS  PubMed  Google Scholar 

  8. Martinez-Cordero, E., Burgos-Vargas, R., Herrera-Esparza, R., Reyes, P.A. Perfil de anticuerpos antinucleares en la esclerosis generalizada progresiva. Estudio multicéntrico. Rev Invest Clin Méx 1983, 35, 273–277.

    CAS  Google Scholar 

  9. Kaburaki, J., Stollar, B.D. Identification of human anti-DNA anti-RNP and anti-Sm and anti-SS-A serum antibodies bearing the cross reactive 16/6 idiotype. J Immunol 1987, 139, 385–392.

    CAS  PubMed  Google Scholar 

  10. Pisetsky, D.S., Hoch, S.O., Katt, C.L., O'Donnell, M.A., Keene, J.D. Specificity and idiotypic analysis of a monoclonal anti-Sm antibody with anti-DNA activity. J Immunol 1985, 135, 4080–4085.

    CAS  PubMed  Google Scholar 

  11. Rubin, R.L., Joslin, F.G., Tan, E.M. An improved ELISA for native DNA by elimination of interference by histone antibodies. J Immunol Meth 1983, 63, 359–366.

    Article  CAS  Google Scholar 

  12. Clark, G., Reichlin, M., Tomasi, T.B. Characterization of a soluble cytoplasmic antigen reactive with sera from patients with systemic lupus erythematosus. J Immunol 1969, 102, 117–122.

    CAS  PubMed  Google Scholar 

  13. Tan, E.M., Kunkel, H.G. Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus. J Immunol 1966, 96, 464–471.

    CAS  PubMed  Google Scholar 

  14. Pettersson, I., Wang, G., Smith, E.F., Wigzell, H., Hedfors, E., Horn, J., Sharp, G.C. The use of immunoblotting and immunoprecipitation of (U) small nuclear ribonucleoproteins in the analysis of sera of patients with mixed connective tissue disease and systemic lupus erythematosus. A cross-sectional, longitudinal study. Arthritis Rheum 1986, 29, 986–996.

    Article  CAS  PubMed  Google Scholar 

  15. Martínez-Cordero, E. Antinuclear antibodies associated with pulmonary involvement in systemic sclerosis. Chest 1989, 96, 960–961.

    Article  PubMed  Google Scholar 

  16. Shoenfeld, Y., André-Schwartz, J., Stollar, B.D., Schwartz R.S. Anti-DNA Antibodies. Chap 7. In: Systemic Lupus Erythematosus. Eds: Lahita, R.G., Kunkel, G.H.. New York: John Wiley and Sons. 1987, 213–255.

    Google Scholar 

  17. Pisetsky, D.S., McCarthy, G.A., Peters, D.V. Mechanisms of autoantibody production in autoimmune MRL mice. J Exp Med 1980, 152, 1302–1310.

    Article  CAS  PubMed  Google Scholar 

  18. Bernstein, R.M., Bunn, C.C., Hughes, C.R.V. Cellular protein and RNA antigens in autoimmune disease. Mol Biol Med 1984, 2, 105–120.

    CAS  PubMed  Google Scholar 

  19. Clotet, B., Guardia, J., Pigraw, C., Lience, E., Murcia, C., Pujol, R., Bacardi, R. Incidence and clinical significance of anti-ENA antibodies in systemic lupus erythematosus. Estimation by counterimmunoelectrophoresis. Scand J Rheumatol 1984, 13, 15–20.

    Article  CAS  PubMed  Google Scholar 

  20. Worrall, J.G., Snaith, M.L., Batchelor, J.R., Isenberg, D.A. SLE a rheumatological view. Analysis of the clinical features, serology and immunogenetics of 100 SLE patients during long term follow-up. Q J Med 1990, 74, 319–330.

    CAS  PubMed  Google Scholar 

  21. Winfield, J.B., Brunner, C.M., Koffler, D. Serologic studies in patients with systemic lupus erythematosus and central nervous system dysfunction. Arthritis Rheum 1978, 21, 289–294.

    Article  CAS  PubMed  Google Scholar 

  22. Beaufils, M., Kouki, F., Mignon, F., Camus, J.P., Morel-Maroger, L., Richet, G. Clinical significance of anti-Sm antibodies in systemic lupus erythematosus. Am J Med 1983, 74, 201–205.

    Article  CAS  PubMed  Google Scholar 

  23. Barada, F.A., Andrew, B.S., Davis, J.S., Taylor, R.P. Antibodies to Sm in patients with systemic lupus erythematosus. Arthritis Rheum 1981, 24, 1236–1244.

    Article  PubMed  Google Scholar 

  24. Reyes, P.A., Tan, E.M. DNA binding property of Sm nuclear antigen. J Exp Med 1977, 145, 749–753.

    Article  CAS  PubMed  Google Scholar 

  25. Darwin, B.S., Grudier, J.P., Klatt, C.L., Pisetsky, D.B. IgG nuclear antibodies with cross reactive rheumatoid factor activity. J Immunol 1986, 137, 3796–3801.

    CAS  PubMed  Google Scholar 

  26. Casali, P. Human lymphocytes making RF and antibody to ssDNA belong to leu 1+B cell subset. Science 1987, 236, 81–82.

    Article  Google Scholar 

  27. Puccetti, A., Koizumi, T., Migliorini, P., Andre-Schwartz, J., Barrett, K., Schwartz, R.S. An immunoglobulin light chain from a lupus prone mouse induces autoantibodies in normal mice. J Exp Med 1990, 171, 1919–1930.

    Article  CAS  PubMed  Google Scholar 

  28. McCarthy, G.A., Rice, J.R., Bembe, M.L., Pisetsky, D.S. Independent expression of autoantibodies in systemic lupus erythematosus. J Rheumatol 1982, 9, 691–695.

    Google Scholar 

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Authors and Affiliations

  1. Unidad de Investigación, Instituto Nacional de Enfermedades Respiratorias, Calzadad de Tlalpan 4502, 14080, México, D.F., México

    E. Martínez-Cordero, E. Martínez-Miranda, M. C. Negrete-García, A. Padilla & D. E. Aguilar León

  2. Unidad de Epidemilogía Clínica, Departamento de Investigación, Hospital 20 de Noviembre, ISSSTE, México

    E. Martínez-Cordero, E. Martínez-Miranda, M. C. Negrete-García, A. Padilla & D. E. Aguilar León

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  1. E. Martínez-Cordero
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  2. E. Martínez-Miranda
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  3. M. C. Negrete-García
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  4. A. Padilla
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  5. D. E. Aguilar León
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Martínez-Cordero, E., Martínez-Miranda, E., Negrete-García, M.C. et al. Anti-dsDNA and Sm autoantibodies in systemic lupus erythematosus. Clin Rheumatol 11, 341–345 (1992). https://doi.org/10.1007/BF02207190

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  • DOI: https://doi.org/10.1007/BF02207190

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