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IL-1 is an important mediator for microcirculatory changes in endotoxin-induced intestinal mucosal damage

  • Intestinal Disorders, Inflammatory Bowel Disease, And Irritable Bowel Syndrome
  • Published:
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Abstract

Although small intestine is frequently injured in endotoxin shock, the exact pathological sequence has not been fully understood. The major objective of this study is to elucidate the role of interleukin (IL)-1 in endotoxin-induced microcirculatory disturbance of rat small intestine. Mucosal and submucosal microvessels of the rat ileum were observed by intravital microscope with a high speed video camera system and the attenuating effect of E5090, an inhibitor of IL-1 generation, on endotoxin-induced intestinal microcirculatory disturbances was investigated. Endotoxin infusion produced significant mucosal damage, but before these morphological changes became significant, microvascular stasis in villi, decreased red blood cell velocity, and increased leukocyte adherence to venular walls were observed in intestinal microcirculatory beds 30 min after endotoxin administration. Intestinal IL-1α levels were also significantly increased at that time. Endotoxin treatment enhanced chemiluminescence activity from neurophils and rapidly mobilized CD18 on leukocytes. E5090, which suppressed the IL-1 production in intestinal mucosa, attenuated the microcirculatory disturbances induced by endotoxin, and significantly reduced the subsequent mucosal damage. E5090 also attenuated the increased chemiluminescence activity and CD18 expression on leukocytes. In conclusion, the production of IL-1α is enhanced in the intestinal mucosa during endotoxin infusion. IL-1 may be an important mediator of microcirculatory changes, including decreased red blood cell velocity and increased leukocyte sticking and its activation, leading to the mucosal damage.

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Authors and Affiliations

  1. From the Departments of Internal Medicine and Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan

    Dai Fukumura MD, Soichiro Miura MD, Iwao Kurose MD, Hajime Higuchi MD, Hidekazu Suzuki MD, Hirotoshi Ebinuma MD, Jing-Yan Han MD, Naoyuki Watanabe MD, Go Wakabayashi MD, Masaki Kitajima MD &  Hiromasa Ishii MD

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  1. Dai Fukumura MD
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  2. Soichiro Miura MD
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  3. Iwao Kurose MD
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  4. Hajime Higuchi MD
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  5. Hidekazu Suzuki MD
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  6. Hirotoshi Ebinuma MD
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  8. Naoyuki Watanabe MD
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  11. Hiromasa Ishii MD
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Fukumura, D., Miura, S., Kurose, I. et al. IL-1 is an important mediator for microcirculatory changes in endotoxin-induced intestinal mucosal damage. Digest Dis Sci 41, 2482–2492 (1996). https://doi.org/10.1007/BF02100147

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  • DOI: https://doi.org/10.1007/BF02100147

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