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Helicobacter pylori potentiates histamine release from rat serosal mast cells induced by bile acids

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Abstract

In the present study we have experimentally addressed the effects ofHelicobacter pylori on the bile acid capability of histamine release. Bile acids alone were confirmed to be able to inducein vitro histamine release from rat serosal and mucosal mast cells. On the contrary, no significant histamine release was obtained when incubating anyHelicobacter pylori preparations alone with mast cells. However, histamine release induced by bile acids was significantly enhanced, without any significant increase in lactate dehydrogenase activity, when whole washed or formalin-killed bacterial cells or crude cell walls were incubated with mast cells in the presence of cholic (0.3 mM), deoxycholic (0.3 mM), or lithocholic (0.3 mM) acids, chenodeoxycholylglycine (0.3 mM), and deoxycholyltaurine (3 mM). The electron microscopic features of mast cells incubated withHelicobacter pylori were consistent with an exocytotic secretion. The release of histamine induced by 0.3 mM deoxycholic acid in the presence ofHelicobacter pylori was inhibited by the preincubation of the cells with dimaprit (an H2 agonist) and potentiated by the H2 antagonist, ranitidine. The current results suggest a link between humanHelicobacter pylori infection and histamine release and a possible involvement of gastric mucosal mast cells in the pathogenesis ofHelicobacter pylori-associated gastritis.

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Authors and Affiliations

  1. From the Istituti di Clinica Chirurgica III e Microbiologia, Dipartimenti di Farmacologia Preclinica e Clinica, Anatomia Umana e Istologia, Universitá di Firenze, Florence, Italy

    E. Masini MD,  P. Bechi MD, R. Dei PhD, M. G. DI Bello MD & T. Bani Sacchi MD

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  1. E. Masini MD
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  2. P. Bechi MD
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  3. R. Dei PhD
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  4. M. G. DI Bello MD
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  5. T. Bani Sacchi MD
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This work was supported by a grant from Ministero dell'Università e della Ricerca Scientifica e Tecnologica.

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Masini, E., Bechi, P., Dei, R. et al. Helicobacter pylori potentiates histamine release from rat serosal mast cells induced by bile acids. Digest Dis Sci 39, 1493–1500 (1994). https://doi.org/10.1007/BF02088054

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  • DOI: https://doi.org/10.1007/BF02088054

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