Summary
The antiperoxidative effects of 35 phenolic compounds, most of them belonging to the flavonoid class, were investigated using CCl4-induced peroxidation of rat liver microsomes. This system was rather insensitive to gallic acid, methyl gallate and ellagic acid. Nevertheless it was inhibited by flavonoids and structure/activity relationships were established. The most potent compounds were gardenin D, luteolin, apigenin (flavones), datiscetin, morin, galangin (flavonols), eriodictyol (flavanone), amentoflavone (biflavone) and the reference compound, (+)-catechin. The natural polymethoxyflavone gardenin D has shown a potency comparable to that of (+)-catechin and higher than that of silybin. Thus, it may be considered as a new type of natural antioxidant with potential therapeutical applications.
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References
Slater, T. F., and Sawyer, B. C., Biochem. J.123 (1971) 805.
Cheeseman, K. H., in: Recent Advances in Lipid Peroxidation and Tissue Injury, p. 86. Eds T. F. Slater and A. Garner. Brunel University Printing Services, Uxbridge 1981.
Cheeseman, K. H., in: Free Radicals, Lipid Peroxidation and Cancer, p. 197. Eds D. C. H. McBrien and T. F. Slater. Academic Press, London 1982.
Poli, G., Albano, E., Biasi, F., Cecchini, G., Carini, R., Bellomo, G., and Dianzani, M. U., in: Free Radicals in Liver Injury, p. 207. Eds G. Poli, K. H. Cheeseman, M. U. Dianzai and T. F. Slater. IRL Press, Oxford 1985.
Dianzani, M. U., and Poli, G., in: Free Radicals in Liver Injury, p. 149. Eds G. Poli, K. H. Cheeseman, M. U. Dianzani and T. F. Slater. IRL Press, Oxford 1985.
Perrissoud, D., and Weibel, I., Naunyn-Schnmiedeberg's Arch. Pharmac.312 (1980) 285.
Slater, T. F., Cheeseman, K. H., Davies, M. J., Proudfoot, K., and Sharma, O. P., in: Free Radicals in Liver Injury. p. 197. Eds G. Poli, K. H. Cheeseman M. U. Dianzani and T. F. Slater. IRL Press, Oxford 1985.
Rodriguez, B., Phytochemistry25 (1977) 800.
Villar, A., Gascó, M. A., Alcaraz, M. J., Máñez, S., and Cortes, D., Planta Med.51 (1985) 70.
Payá, M., Máñez, S., and Villar, A., Z. Naturforsch.41c (1986) 976.
Lowry O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem.193 (1951) 265.
Mansuy, D., Sassi, A., Dansette, P. M., and Plat, M., Biochem. biophys. Res. Commun.135 (1986) 1015.
Osawa, T., Ide, A., Su, J.-D., and Namiki, M. J., Agric. Food Chem.35 (1987) 808.
Scott, R., and Slater, T. F., in: Recent Advances in Lipid Peroxidation and Tissue Injury, p. 233. Eds T. F. Slater and A. Garner. Brunel University Printing Services, Uxbridge 1981.
Ratty, A. K., and Das, N. P., Biochem. Med. Betab. Biol.39 (1988) 69.
Younes, M., and Siegers, C. P., in: Free Radicals in Liver Injury, p. 87. Eds. G. Poli, K. H. Cheeseman, M. U. Dianzani and T. F. Slater. IRL Press, Oxford 1985.
Afanas'ev, I. B., Dorozhko, A. I., Brodskii, A. V., Kostyuk, V. A., and Potapovitch, A. I., Biochem. Pharmac.38 (1989) 1763.
Slater, T. F., and Eakins, M. N., in: New Trends in The Therapy of Liver Diseases. p. 84. Ed. A. Bertelli. Karger, Basel 1975.
Slater, T. F., in: Free Radicals, Lipid Peroxidation and Cancer, p. 243. Eds D. C. H. McBrien and T. F. Slater. Academic Press, London 1982.
Torel, J., Cillard, J., and Cillard, P., Phytochemistry25 (1986) 383.
Sousa, R. L., and Marletta, M. A., Archs Biochem. Biophys.240 (1985) 345.
Havsteen, B., Biochem. Pharmac.32 (1983) 1141.
Campos, R., Garrido, A., Guerra, R., and Valenzuela, A., in: Plant Flavonoids in Biology and Medicine II. Biochemical, Cellular and Medicinal Properties, p. 375. Eds V. Cody, E. Middleton Jr, J. B. Harborne and A. Beretz. Alan R. Liss Inc., New York 1988.
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Cholbi, M.R., Paya, M. & Alcaraz, M.J. Inhibitory effects of phenolic compounds on CCl4-induced microsomal lipid peroxidation. Experientia 47, 195–199 (1991). https://doi.org/10.1007/BF01945426
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DOI: https://doi.org/10.1007/BF01945426