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Coronary collateral reserve during exercise induced ischemia in swine

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Summary

We determined coronary collateral vasodilator reserve during exercise-induced ischemia in 17 minis-wine. We induced coronary collateral development in the left circumflex bed by placing an ameroid occluder on that artery. Four weeks later we studied the animals at rest and during exercise (EX) eliciting heart rates (HR) of 240 and 265 beats/min. We measured myocardial blood flow with microspheres and moyocardial function by wall thickness sonomicrometry gauges. At matched exercise HRs we treated the animals with nifedipine (10 μg/kg IV) (EXN 10), nifedipine (100 μg/kg IV), (EXN 100), and adenosine infusion (1.2 mg/min/kg) EXAD. EXN 10 did not significantly alter hemodynamics compared to EX but EXN 100 and EXAD both decreased blood pressure significantly (p<0.05). Ischemic endocardial/nonischemic endocardial flow ratios and collateral resistance served as indices of vasodilator reserve. In the ischemic zone exercise reduced vasodilator reserve to 24±3% in the endocardium and 64±7% in the epicardium. Neither EXN 10 nor EXAD improved exercise-induced ischemia measured either as flow or function. However EXN 100 improved function during exercise-induced ischemia without improving coronary collateral flow. We conclude there is no additional coronary flow reserve during exercise-induced ischemia in the collateral dependent bed of the pig a few days after occlusion that can be recruited. Large doses of nifedipine improve function by direct action on the myocardium or by reducing afterload. The lack of development and deep myocardial distribution of the coronary collateral vessels in the pig may be an important factor of why these nifedipine responses differ from those reported in species which have primarily large epicardial coronary collaterals.

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References

  1. Aversano T, Becker LC (1985) Persistence of coronary vasodilator reserve despite functionally significant flow reduction. Am J Physiol 248:H403-H411

    PubMed  Google Scholar 

  2. Bloor CM, White FC, Magnet A (1988) Anatomic features of coronary collateral development in swine. Lab Invest 58 (1):59

    Google Scholar 

  3. Canty JM, Klocke FJ (1985) Reduced regional myocardial perfusion in the presence of pharmacologic vasodilator reserve. Circulation 71:370–377

    PubMed  Google Scholar 

  4. Dymek DJ, Bache RJ (1984) Effects of nifedipine and diltiazem on coronary reactive hyperaemia. Cardiovasc Res 18:249–256

    PubMed  Google Scholar 

  5. Gallagher KP, Kumada T, Koziol JA, McKown MD, Kemper WS, Ross J Jr (1980) Significance of regional wall thickening abnormalities relative to transmural myocardial perfusion in anesthetized dogs. Circulation 62:1266–1274

    PubMed  Google Scholar 

  6. Gunther S, Green L, Muller JE, Mudge GH, Grossman W (1981) Prevention by nifedipine of abnormal coronary vasoconstriction in patients with coronary artery disease. Circulation 63:849–855

    PubMed  Google Scholar 

  7. Guth BD, Tijimi T, Seitelberger R, Lee JD, Matsuzaki M, Ross J Jr (1986) Experimental exercise-induced ischemia: drug therapy can eliminate regional dysfunction and oxygen supply-demand imbalance. J Am Coll Cardiol 7:1036–1044

    PubMed  Google Scholar 

  8. Henry PD, Shuchleib R, Borda LJ, Roberts R, William JR, Sobel BE (1978) Effects of Nifedipine on myocardial perfusion and ischemic injury in dogs. Circ Res 43:372–380

    PubMed  Google Scholar 

  9. Heusch G, Deussen A (1984) Nifedipine prevents sympathetic vasoconstriction distal to severe coronary stenoses. J Cardiovasc Pharm 6:378–383

    PubMed  Google Scholar 

  10. Heusch G, Guth BD, Steitelberger R, Ross J Jr (1987) Recruitment of coronary reserve by nifedipine attenuates exercise-induced myocardial ischemia in dogs. Circulation 75:482–490

    PubMed  Google Scholar 

  11. Heymann MA, Payne BD, Hoffman JIE, Rudolph AM (1977) Blood flow measurements with radionuclide-labeled particles. Prog Cardiovasc Dis 20:55–79

    PubMed  Google Scholar 

  12. Matsuzaki M, Gallagher KP, Patritti J, Tajimi T, Kemper WS, White FC, Ross J Jr (1984) Effects of a calcium-entry blocker (diltiazem) on regional myocardial flow and function during exercise in conscious dogs. Circulation 69 (4):801–814

    PubMed  Google Scholar 

  13. Millard R (1980) Changes in cardiac mechanics and coronary blood flow of regionally ischemic porcine myocardium induced by Diltiazem. Chest 78:193–199

    PubMed  Google Scholar 

  14. O'Konski MS, White FC, Longhurst JR, Roth DM, Bloor CM (1987) Ameroid constriction of the proximal left circumflex coronary artery in swine. Am J Cardiovasc Path 1:69–77

    Google Scholar 

  15. Pantely GA, Bristow JD, Swenson LJ, Ladley HD, Johnson WB, Anselone GG (1985) Incomplete coronary vasodilation during myocardial ischemia in swine. Am J Physiol 249:H638-H647

    PubMed  Google Scholar 

  16. Roth DM, Mauroka Y, Rogers J, White FC, Longhurst JC, Bloor CM (1987) Development of the coronary collateral circulation in left circumflex ameroid occluded swine myocardium. Am J Physiol 253:H1279–1288.

    PubMed  Google Scholar 

  17. Roth DM, White FC, Mathieu-Costello O, Guth BD, Heusch G, Bloor CM, Longhurst JC (1987) Effects of left circumflex ameroid constrictor placement on adrenergic innervation of the myocardium. Am J Physiol

  18. Schaper W, Wusten B, Flameng W, Scholtholt J, Winkler B, Pasyk D (1975) Local dilatory reserve in chronic experimental coronary occlusion without infarction. Quantitation of collateral development. Bas Res Cardiol 70:159–173

    Google Scholar 

  19. Schulz W, Jost S, Kober G, Kaltenbach M (1985) Relation of antianginal efficacy of nifedipine to degree of coronary arterial narrowing and to presence of coronary collateral vessels. Am J Cardiol 55:26–32

    PubMed  Google Scholar 

  20. Sjoquist P-O, Duker G, Almgren O (1985) Coronary collateral blood flow in acute myocardial ischemia is not increased by Dxydropyridine-induced coronary vasodilatation. J Cardiovasc Pharmacology 7:630–636

    Google Scholar 

  21. Specchia G, DeServi S, Falcone C, Angoli L, Gavazzi A, Bramucci E, Mussini A, Ferrario M, Salerno J, Montemartini C (1983) Effects of nifedipine on coronary hemodynamic findings during exercise in patients with stable exertional angina. Circulation 68:1035–1043

    PubMed  Google Scholar 

  22. Vatner SF, Hintze TH (1982) The effects of a calcium-channel antagonist on large and small coronary arteries in conscious dogs. Circulation 66:579–588

    PubMed  Google Scholar 

  23. Warltier DC, Gross EJ, Brooks HL (1981) Pharmacology—vs ischemic-induced coronary vasodilation. Am J Physiol 9:H767-H774

    Google Scholar 

  24. Weintraub WS, Hattori S, Agarwal J, Bodenheimer MM, Banka VS, Helfant RH (1981) Variable effects of nifedipine on myocardial blood flow at three grades of coronary occlusion in the dog. Circ Res 48:937–942

    PubMed  Google Scholar 

  25. Weintraub WS, Hattori S, Agarwal JB, Bodenheimer MM, Benka VS, Helfant RH (1982) The effects of nifedipine on myocardial blood flow and contraction during ischemia in the dog. Circ 65:49–63

    Google Scholar 

  26. White FC, Bloor CM (1981) Coronary collateral circulation in the pig: Correlation of collateral flow and coronary bed size. Basic Res Cardiol 76:189–196

    PubMed  Google Scholar 

  27. Zacca NM, Verani MS, Chahine RA, Miller RR (1982) Effect of nifedipine on exercise-induced left ventricular dysfunction and myocardial hypoperfusion in stable angina. Am J Cardiol 50:689–698

    PubMed  Google Scholar 

  28. Zyvoloski MG, Brooks HL, Gross GJ, Warltier DC (1982) Myocardial perfusion distal to an acute or chronic coronary artery occlusion: effects of Diltiazem and nifedipine. The J of Pharm and Exp Therap 2:494–500

    Google Scholar 

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Author notes

  1. F. C. White (Research Pathologist)

    Present address: School of Medicine Department of Pathology, University of California San Diego, M-012, 92093, La Jolla, California, U.S.A.

Authors and Affiliations

  1. School of Medicine Department of Pathology, University of California San Diego, M-012, 92093, La Jolla, California, U.S.A.

    D. M. Roth & C. M. Bloor

Authors
  1. F. C. White
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  2. D. M. Roth
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  3. C. M. Bloor
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Grant Support: NHLBI HL-32670

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White, F.C., Roth, D.M. & Bloor, C.M. Coronary collateral reserve during exercise induced ischemia in swine. Basic Res Cardiol 84, 42–54 (1989). https://doi.org/10.1007/BF01907002

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  • DOI: https://doi.org/10.1007/BF01907002

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