Summary
Descending rabbit colon, stripped ofmuscularis externa, absorbs Na and Cl under short-circuit conditions and exhibits a residual ion flux, consistent with HCO3 secretion, whose magnitude is approximately equal to the rate of active Cl absorption. Net K transport was not observed under short-circuit conditions. The results of ion replacement studies and of treatment with ouabain or amiloride suggest that the short-circuit currentI sc is determined solely by the rate of active Na transport and that the net movements of Cl and HCO3 are mediated by a Na-independent, electrically-neutral, anion exchange process. Cyclic AMP stimulates an electrogenic Cl secretion, abolishes HCO3 secretion but does not affect the rate of Na absorption under short-circuit conditions. Studies of the effect of transepithelial potential difference on the serosa-to-mucosa fluxesJ i sm of Na, K and Cl suggest thatJ Na sm ,J K sm and one-third ofJ Cl sm may be attributed to ionic diffusion. The permeabilities of the passive conductance pathway(s) are such thatP K∶P Na∶P Cl=1.0∶0.07∶0.11. Electrolyte transport byin vitro rabbit colon closely resembles that reported fromin vivo studies of mammalian colon and thus may serve as a useful model for the further study of colonic ion transport mechanisms.
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Frizzell, R.A., Koch, M.J. & Schultz, S.G. Ion transport by rabbit colon. J. Membrain Biol. 27, 297–316 (1976). https://doi.org/10.1007/BF01869142
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DOI: https://doi.org/10.1007/BF01869142