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Isolation and characterization of a bacteriophage forBacillus licheniformis A5

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Abstract

The isolation of a virulent bacteriophage forBacillus licheniformis A5 is reported. This bacteriophage, designated NLP-1, has an icosahedral head 100 nm in diameter and a contractible tail with a maximum length of 130 nm. Its DNA has a density of 1.741 g/cm3 and aT m of 78.4°C. Base composition analysis showed that thymine is absent and is replaced by hydroxymethyluracil. NLP-1 appears to belong to theBacillus group of bacteriophages that includes SP01 and SP82. It will infectB. cereus T andB. brevis 8185, but will not infectB. subtilis W23 or 168.

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Authors and Affiliations

  1. Program in Biochemistry, The Pennsylvania State Univeristy, 16802, University Park, Pennsylvania, USA

    Harold J. Schreier & Robert W. Bernlohr

  2. Program in Microbiology, The Pennsylvania State University, 16802, University Park, Pennsylvania, USA

    Eillen K. Vonada & Ronald E. Yasbin

Authors
  1. Harold J. Schreier
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  2. Eillen K. Vonada
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  3. Ronald E. Yasbin
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  4. Robert W. Bernlohr
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Schreier, H.J., Vonada, E.K., Yasbin, R.E. et al. Isolation and characterization of a bacteriophage forBacillus licheniformis A5. Current Microbiology 7, 103–106 (1982). https://doi.org/10.1007/BF01568423

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