Summary
Nests of cells within the central nervous system, namely the circumventricular organs (CVOs) which include the subfornical organ (SFO), organum vasculosum lamina terminalis (OVLT), area postrema (AP) and the median eminence (ME) are known to contain not only receptors for angiotensin II (ANG II) but also ANG II itself. Though the significance of this central ANG II network in the pathophysiology of certain conditions like hypertension is well established, there appears to be a lack of knowledge as to how this system might be involved after subarachnoid haemorrhage (SAH). In this study, we have investigated ANG II receptor content change at various circumventricular organs after experimental subarachnoid haemorrhage in rats using a transcervical transclivai model. ANG II receptor content was detected by in vivo autoradiography using intracisternal ANG II Sar 1, Ile 8 labelled with iodine (I) 125 both at 30 minutes and 48 hours after the SAH. Serum angiotensin converting enzyme activity was also detected during the time course reflecting the involvement of the peripheral angiotensin system and showed an early rise and a fall after two days. Immunohistochemistry was utilized to show the ANG II-containing cells within the circumventricular organs. SFO and OVLT were found to have a statistically significant increase in ANG II receptor content persisting over two days after the SAH. These alterations in the receptor content of CVOs may indicate their possible role in delayed ischaemic deficits seen after SAH.
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References
Barry KJ, Gogjian MA, Stein BM (1979) Small animal model for investigation of subarachnoid hemorrhage and cerebral vasospasm. Stroke 10: 538–541
Bennett J, Snyder SH (1980) Receptor binding interactions of the angiotensin II antagonist, I-125 (Sarcosine 1, Leucine 8) angiotensin II, with mammalian brain and peripheral tissues. Eur J Pharmacol 67: 11–25
Bennett JP, Snyder SH (1976) Angiotensin II binding to mammalian brain membranes. J Biol Chem 251: 7423–7430
Berney J (1986) Subarachnoid hemorrhage. Clinical evaluation and natural history. Presented at 3rd EANS Course, Porto, Portugal, September 6–12
Borison HL (1984) History and status of the area postrema. Federation Proceedings 43: 2937–2940
Brownfield MS, Reid LA, Ganten D,et al (1982) Differential distribution of immunoreactive angiotensin and angiotensine converting enzyme in rat brain. Neuroscience 7: 1759–1769
Brunner H, Chang P, Wallach,et al (1972) Angiotensin II vascular receptors. Their avidity in relationship to sodium balance, the autonomic nervous system and hypertension. J Clin Invest 51: 58–67
Changaris DG, Severs WB, Keil LC (1978) Localization of angiotensin in rat brain. J Histochem Cytochem 26: 593–607
Delgado TJ, Brismar J, Svendgaard NA (1985) Subarachnoid hemorrhage in the rat. Angiography and flourescence microscopy of the major cerebral arteries. Stroke 16: 595–601
Editorial in Ganong WF, Martini L (eds) (1990) Frontiers in Neuroendocrinology, vol 11. Raven, New York, pp 1–5
Edvinsson L, Uddman R, Juul R (1990) Peptidergic innervation of the cerebral circulation. Role in subarachnoid hemorrhage in man. Neurosurg Rev 13: 265–272
Ferguson AV, Kasting NW (1986) Electrical stimulation in subfornical organ increases plasma vasopressin concentrations in the conscious rat. Am J Physiol 251: R425–428
Gavras H, Andrews P, Papadakis N (1981) Reversal of experimental delayed cerebral vasospasm by angiotensin converting enzyme inhibition. J Neurosurg 55: 884–888
Harding JW, Stone LP, Wright JW (1981) The distribution of angiotensin binding sites in the rodent brain. Brain Res 205: 265–274
Haywood JP, Fink GD, Buggy J,et al (1980) The area postrema plays no role in the pressor action of angiotensin in the rat. Am J Physiol 239: H108–113
Heros RC, Zervas NT, Varsos V (1983) Cerebral vasospasm after subarachnoid hemorrhage and update. Ann Neurol 14: 599–608
Israel A, Correa FMA, Niwa M,et al (1984) Quantitative determination of angiotensin II binding sites in rat brain and pituitary gland by autoradiography. Brain Res 322: 341–345
Kassell NF, Sasaki T, Colohan ART (1985) Cerebral vasospasm following aneurysm subarachnoid hemorrhage. Stroke 16: 562–572
Lind RW, Swanson LW, Ganten D (1985) Organization of angiotensin II immunoreactive cells and fibers in the rat central nervous system. Neuroendocrinology 40: 2–24
Mangiapane ML, Simpson JB (1980) Subfornical organ: forebrain site of pressor and dipsogenic action of angiotensin II. Am J Physiol 239: R382–389
Mann JFE, Johnson AK, Ganten D (1980) Plasma angiotensin II: dipsogenic levels and angiotensin-generating capacity of renin. Am J Physiol 238: R372–377
Nazarali AJ, Gutkind JS, Correa FMA,et al (1989) Enapril decreases angiotensin II receptors in subfornical organ of SHR. Am J Physiol 256: H1609–1614
Neil-Dwyer G, Cruickshank JM, Doshi R (1990) The stress response in subarachnoid hemorrhage. Acta Neurochir (Wien) [Suppl] 47: 102–110
Phillips MI, Stamler JF, Raizada MK (1980) Visualization of specific angiotensin II binding sites in the brain by fluorescent microscopy. Science 210: 791–793
Quinlan JT, Phillips MI (1981) Immunoreactivity for an angiotensin II-like peptide in the human brain. Brain Res 205: 212–218
Ray PE, Ruley EJ, Saavedra JM (1990) Down-regulation of angiotensin receptors in subfornical organ of young male rats chronic dietary sodium depletion. Brain Res 510: 303–308
Robertson AL, Khariallah PA (1971) Angiotensin II: rapid localization in nuclei of smooth cardiac muscle. Science 172: 1138–1139
Severs WB, Summy-Long JY, Taylor CS,et al (1970) A central effect of angiotensin: release of a pituitary pressor material. J Pharmacol Exp Ther 174: 27–34
Sirett NE, McLean AS, Bray JJ,et al (1977) Distribution of angiotensin receptors in rat brain. Brain Res 122: 299–312
Sirett NE, Thornton SN, Hubbard JI (1979) Angiotensin binding and pressor activity in the rat ventricular system and midbrain. Brain Res 166: 139–148
Spetzler RF, Selman WR (1980) New design for an implantable vessel occluder. Surg Neurol 13: 317–319
Svendgaard N (1988) Discussion on anterior circulation aneurysm. In: Suzuki J (ed) Advances in surgery for cerebral stroke. Springer, Tokyo, p 299
Verlooy J, Reempts JV, Haseldonckx M,et al (1992) Haemodynamic, intracranial pressure and electrocardiographic changes following subarachnoid hemorrhage in rats. Acta Neurochir (Wien) 115: 118–122
Weindl A (1973) Neuroendocrine aspects of circumventricular organs. In: Ganong WF, Martini L (eds) Frontiers in neuroendocrinology, vol 3. Oxford University Press, London, pp 3–32
Weindl A, Joynt RJ (1973) Subcommissural organ. Arch Neurol 29: 17–22
Weindl A, Joynt RJ (1972) Ultrastructure of the ventricular walls. Arch Neurol 26: 420–427
Wright JW, Harding JW (1992) Regulatory role of brain angiotensins in the control of physiological and behavioral responses. Brain Res Brain Res Rev 17: 227–262
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Açikgöz, B., Özgen, T., Özdoğan, F. et al. Angiotensin II receptor content within the subfornical organ and organum vasculosum lamina terminalis increases after experimental subarachnoid haemorrhage in rats. Acta neurochir 138, 460–465 (1996). https://doi.org/10.1007/BF01420309
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DOI: https://doi.org/10.1007/BF01420309