Summary
In this paper methods for ABO blood grouping of saliva from mixed body fluids have been established. Monoclonal antibodies to tissue specific epitopes on blood group substances in saliva were used as solid phase antibodies to catch the blood group substances. ABO blood grouping of saliva could be performed by these methods without interference from other body fluids (eg. semen, vaginal secretion, urine, sweat and serum). At least 16,000 and 3,000 fold dilutions of secretor saliva were sufficient for ABO blood grouping by sandwich ELISA and sandwich absorption-elution test, respectively.
Zusammenfassung
In dieser Untersuchung wurden Methoden zur ABO-Blutgruppenbestimmung an Speichel aus gemischten Körperflüssigkeiten etabliert. Monoklonale Antikörper zu gewebsspezifischen Epitopen für Blutgruppensubstanzen in Speichel wurden als „solid phase” Antikörper benutzt, um Blutgruppensubstanzen zu binden. Die ABO-Blutgruppenbestimmung von Speichel konnte mit diesen Methoden ohne Interferenz mit anderen Körperflüssigkeiten (z. B. Sperma, Vaginalsekret, Urin, Schweiß und Serum) durchgeführt werden. Speichelverdünnungen von Sekretoren zwischen 3.000fach und 16.000fach waren ausreichend für die ABO-Blutgruppenbestimmung mit Hilfe von „Sandwich-ELISA” und bzw. „Sandwich-Absorptions-Elutions-Test”.
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References
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Kimura, A., Matsumura, F., Sodesaki, Ki. et al. ABO blood grouping of saliva from mixed body fluids by sandwich methods using monoclonal antibodies to tissue specific epitopes on blood group substance in saliva. Int J Leg Med 104, 189–192 (1991). https://doi.org/10.1007/BF01369804
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DOI: https://doi.org/10.1007/BF01369804