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Insulinlike growth factor gene expression in human fracture callus

  • Clinical Investigations
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Summary

The effects of insulinlike growth factors on bone and cartilage-derived cells in culture have been extensively investigated, but there is little information on their rolein vivo in bone, especially in fracture healing. This study investigated insulinlike growth factor (IGF) I and II mRNA expression in normally healing human fractures byin situ hybridization. Endothelial and mesenchymal cells at the granulation tissue stage expressed IGF-II mRNA. At the stage of bone and cartilage formation, osteoblasts and nonhypertrophic chondrocytes expressed mRNA for both IGF-I and II. Some osteoclasts were positive for IGF-II mRNA at the stage of bone remodeling. The greater time span of IGF-II expression relative to IGF-I reflects the predominance of IGF-II in human bone matrix. Taken together with the known effects of IGFs on bone and cartilage cellsin vitro, these findings support a role for IGFs in local cellular regulation in human fracture healing.

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Authors and Affiliations

  1. Department of Orthopaedics, Clinical Sciences Building, Hope Hospital, Eccles Old Road, M6 8HD, Salford, UK

    J. G. Andrew & D. Marsh

  2. Department of Pathological Sciences, University of Manchester Medical School, Oxford Road, M13 9PT, Manchester, UK

    J. Hoyland & A. J. Freemont

Authors
  1. J. G. Andrew
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  2. J. Hoyland
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  3. A. J. Freemont
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  4. D. Marsh
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Andrew, J.G., Hoyland, J., Freemont, A.J. et al. Insulinlike growth factor gene expression in human fracture callus. Calcif Tissue Int 53, 97–102 (1993). https://doi.org/10.1007/BF01321886

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  • DOI: https://doi.org/10.1007/BF01321886

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