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Thermostability of Newcastle disease virus strains of different virulence

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Summary

The thermal inactivation rate constant for infectivity of a total of 24 Newcastle disease virus (NDV) strains was determined at 50° and 56° C. The greater part of the examined NDV strains were found, irrespective of virulence properties, to be thermostable, since the loss of infectivity titre did not exceed 2 logarithmic orders after exposure at 50° C for 60 minutes.

Thermostable (I+) and thermolabile (I−) strains with respect to infectivity were uniformly encountered among the 12 avirulent (lentogenic) and 10 fully virulent (velogenic) strains studied. Strains with both heat stable (Ha+) and heat labile (Ha−) haemagglutinin were found in the lentogenic group, whereas haemagglutinins of all examined velogenic strains were heat stable. On the basis of I/Ha character,i.e. combination of thermosensitivity of infectivity and haemagglutinin, all examined strains could be classified into one of three categories: I−Ha− (8 lentogenic strains), I+Ha+ (4 lentogenic and 7 velogenic strains) and I−Ha+ (2 mesogenic and 3 velogenic strains). The possible fourth combination, I+Ha−, was not encountered among the strains studied. Determination of the I/Ha character may be helpful in clarifying the origin of a lentogenic NDV strain.

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  1. Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary

    B. Lomniczi

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Lomniczi, B. Thermostability of Newcastle disease virus strains of different virulence. Archives of Virology 47, 249–255 (1975). https://doi.org/10.1007/BF01317812

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  • DOI: https://doi.org/10.1007/BF01317812

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