Summary
Induction of microtubulin paracrystals (Pc) by Kunjin (KUN) virus occurred after 15 hours post-infection and were often associated with convoluted membranes (cm) and virus particles. Vinblastine sulphate which disrupts microtubulin, had an inhibitory effect on the virus production when added during the viral latent period. When infected samples were extracted with Triton X-100 and analysed by SDS-PAGE, four viral proteins were observed.
This is a preview of subscription content, log in via an institution to check access.
References
Bensch KG, Malawista SE (1969) Microtubular crystals in mammalian cells. J Cell Biol 40: 95–107
Clewley JP, Kennedy SIT (1976) Purification and polypeptide composition of Semliki Forest virus RNA polymerase. J Gen Virol 32: 395–411
Dales S, Chardonnet Y (1973) Early events in the interaction of adenoviruses with HeLa cells IV. Association with microtubules and the nuclear pore complex during vectorial movement of the inoculum. Virology 56: 456–483
Fulton AB, Wan KM, Penman S (1980) The spatial distribution of polyribosomes in 3T3 cells and the associated assembly of proteins into the skeleton framework. Cell 20: 849–857
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Ng ML, Pederson JS, Toh BH, Westaway EG (1983) Immunofluorescent sites in Vero cells infected with the flavivirus Kunjin. Arch Virol 78: 177–190
Ng ML (1987) Ultrastructural studies of Kunjin virus-infectedAedes albopictus cells. J Gen Virol 68: 577–582
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hong, S.S., Lee Ng, M. Involvement of microtubules in Kunjin virus replication. Archives of Virology 97, 115–121 (1987). https://doi.org/10.1007/BF01310739
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310739