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A recombinant fowlpox virus that expresses the VP2 antigen of infectious bursal disease virus induces protection against mortality caused by the virus

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Summary

The coding sequences of VP 2 from a virulent strain, 52/70, of infectious bursal disease virus (IBDV) were excised from a cDNA clone and inserted into a fowlpox plasmid insertion vector. The resulting plasmid, pIBD 1, was used to construct a recombinant fowlpox virus, fpIBD 1, which expressed VP 2 as a β-galactosidase fusion protein. Chickens vaccinated with fpIBD 1 at 1 and 14 days of age, were challenged at 28 days with either IBDV strain 52/70 or the highly virulent strain CS 89. These chickens were protected against mortality, but not against damage to the bursa of Fabricius. The protection achieved by the use of fpIBD 1 shows that VP 2 is a host protective antigen.

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Author notes

  1. M. M. Binns

    Present address: Equine Virology Unit, Animal Health Trust, Newmarket, Suffolk, U.K.

  2. M. E. G. Boursnell

    Present address: Immunology Ltd, Cambridge, U.K.

Authors and Affiliations

  1. Houghton Laboratory, AFRC Institute for Animal Health, Huntingdon, U.K.

    C. D. Bayliss, R. W. Peters, Jane K. A. Cook, R. L. Reece, K. Howes, M. M. Binns & M. E. G. Boursnell

Authors
  1. C. D. Bayliss
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  2. R. W. Peters
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  3. Jane K. A. Cook
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  4. R. L. Reece
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  5. K. Howes
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  6. M. M. Binns
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  7. M. E. G. Boursnell
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Bayliss, C.D., Peters, R.W., Cook, J.K.A. et al. A recombinant fowlpox virus that expresses the VP2 antigen of infectious bursal disease virus induces protection against mortality caused by the virus. Archives of Virology 120, 193–205 (1991). https://doi.org/10.1007/BF01310475

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  • DOI: https://doi.org/10.1007/BF01310475

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