Abstract
Dopamine and serotonin were determined in extracellular fluid of rat striatum by semiderivativein vivo voltammetry during normoxia and a single or repeated exposure to 15% O2 (i.e., mild hypoxia) or 12.5% O2 (i.e., moderate hypoxia). A single exposure to 15% oxygen increased extracellular dopamine 76%. With reintroduction of air to the animals, dopamine values returned to baseline. During a second episode of 15% oxygen, dopamine increased 63% and remained elevated even during a final exposure to air. On the other hand, serotonin was unaffected by 15% oxygen. Moderate hypoxia (12.5% oxygen) increased dopamine (79%) and serotonin (26%) and both remained elevated even after the initial reintroduction of air. These studies demonstrate thatin vivo hypoxia increases rat striatal extracellular dopamine and, to a lesser extent, extracellular serotonin. Furthermore, after repeated, mild hypoxic episodes or moderate hypoxia, the increases in rat striatal extracellular dopamine and serotonin continue even during normoxia. These studies further support a role for dopamine and serotonin in hypoxic-induced changes in brain function. The hypoxic-induced elevation of these two neurotransmitters during normoxia may be important in the production of hypoxic/ischemic-induced cell damage.
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Altman, P. L. (1961). In D. S. Dittman; (ed.),Blood and Other Body Fluids, Fed. Am. Soc. Exp. Biol., Washington, D.C., p. 186.
Biggio, G., Casu, M., Corda, M. G., Dibello, C., and Gessa, G. L. (1978). Stimulation of dopamine synthesis in caudate nucleus by intrastriatal enkephalins and antagonism by naloxone.Science 200: 552–554.
Broderick, P. A. (1986). Rat striatal dopamine release mechanisms of cocaine. In Holaday, J. W., Law, P. Y., and Herz, A. (eds.),Progress in Opioid Research, National Institute on Drug Abuse Monograph 75, pp. 367–370.
Broderick, P. A. (1987). Striatal neurochemistry of dynorphin-(1–13):In vivo electrochemical semi-differential analyses.Neuropeptides 10: 369–386.
Broderick, P. A. (1988). Distinguishingin vitro electrochemical signatures for norepinephrine and dopamine.Neurosci. Lett. 95: 275–280.
Broderick, P. A. (1989). Characterizing stearate probesin vitro for the electrochemical detection of dopamine and serotonin.Brain Res. (in press).
Brown, R. M., Snider, S. R., and Carlsson, A. (1974). Changes in biogenic amine synthesis and turnover induced by hypoxia and or foot shock stress. II. The central nervous system.J. Neural. Transm. 35: 293–305.
Brown, R. M., Kehr, W., and Carlsson, A. (1975). Functional and biochemical aspects of catecholamine metabolism in brain under hypoxia.Brain Res. 85: 491–509.
Clemens, J. A., and Phebus, L. A. (1988). Dopamine depletion protects striatal neurons from ischemia-induced cell death.Life Sci. 42: 707–713.
Davis, J. N., and Carlsson, A. (1973). Effect of hypoxia on tyrosine and tryptophan hydroxylation in unanesthetized rat brain.J. Neurochem. 20: 913–915.
Diament, M. L., and Palmer, K. N. V. (1966). Postoperative changes in gas tensions of arterial blood and ventilatory function.Lancet 2: 180.
Freeman, G. B., and Gibson, G. E. (1986). Effect of decreased oxygen onin vitro release of endogenous dopamine from mouse striatum.J. Neurochem. 47: 1924–1931.
Freeman, G. B., Nielsen, P., and Gibson, G. E. (1986). Monoamine neurotransmitter metabolism and locomotor activity during chemical hypoxia.J. Neurochem. 46: 733–738.
Gibson, G. E., Peterson, C., and Sansone, J. (1981). Decreases in amino acid and acetylcholine metabolism during hypoxia.J. Neurochem. 37: 192–201.
Globus, M. T. T., Ginsberg, M. D., Harik, S. I., Busto, R., and Kietrich, W. D. (1987). Role of dopamine in ischemic striatal injury: Metabolic evidence.Neurology 37: 1712–1719.
Hirsch, J. A., and Gibson, G. E. (1984). Selective alteration of neurotransmitter release by low oxygenin vitro.Neurochem. Res. 9: 1039–1049.
Lee, E. H., and Geyer, M. A. (1984). Dopamine autoreceptor mediation of the effects of apomorphine on serotonin neurons.Pharm. Biochem. Behav. 21: 301–311.
Lillie, R. D. (ed.) (1977).H. J. Conn's Biological Stains, 9th ed., Williams and Wilkens, Baltimore, Md., p. 412.
Miwa, S., Fujiwara, M., Inoue, M., and Fujiwara, M. (1986). Effects of hypoxia on the activities of noradrenergic and dopaminergic neurons in the rat brain.J. Neurochem. 47: 63–69.
Mrsulja, B. B., Mrsulja, B. J., Spatz, M., and Klatzo, I. (1976). Catecholamines in brain ischemia-effects of alpha-methyl-p-tyrosine and pargyline.Brain Res. 104: 373–378.
Nunn, J. F., and Payne, J. P. (1962). Hypoxemia after general anesthesia.Lancet 2: 631.
Pastuszko, A., Wilson, D. F., and Erecinska, M. (1982). Neurotransmitter metabolism in rat brain synaptosomes: Effect of anoxia and pH.J. Neurochem. 38: 657–1667.
Pelligrino, L. J., and Cushman, A. J. (1967).A Sterotaxic Atlas of the Rat Brain, Appleton-Century-Crofts, New York, p. 19.
Peterson, C., and Gibson, G. E. (1982). 3,4-Diaminopyridine alters acetylcholine metabolism and behavior during hypoxia.J. Pharmacol. Exp. Ther. 22: 576–582.
Phebus, L. A., Perry, K. W., Clemens, J. A., and Fuller, R. W. (1986). Brain anoxia releases striatal dopamine in rats.Life Sci. 38: 2447–2453.
Prioux-Guyonneau, M., Cretet, E., Jacquot, C., Rapin, J. R., and Cohen, Y. (1979). The effect of various simulated altitudes on the turnover of norepinephrine and dopamine in the central nervous system of rats.Pflugers Arch. 380: 127–132.
Rice, M. E., Oke, A. F., Bradberry, C. W., and Adams, R. N. (1985). Simultaneous voltammetric and chemical monitoring of dopamine releasein situ.Brain Res. 340: 151–155.
Sandler, J. (1955). A test of the significance of the difference between the means of correlated measures based on a simplification of Student's t.Br. J Psychol. 46: 225–226.
Shimada, M., Kihara, T., Kurimoto, K., and Watanabe, M. (1974). Incorporation of14C from [U-14C]-sglucose into free amino acids under cyanide intoxication.J. Neurochem. 23: 379–384.
Silverstein, F., and Johnston, M. V. (1984). Effects of hypoxia-ischemia on monoamine metabolism in the immature brain.Ann. Neurol. 15: 342–347.
Steel, R. G. D., and Torrie, J. H. (1960).Principles and Procedures of Statistics, McGraw-Hill, New York, pp. 99–107.
Trouvin, J. H., Prioux-Guyonneau, M., Cohen, Y., and Jacquot, C. (1986). Rat brain monoamine metabolism and hypobaric hypoxia: A new approach.Gen. Pharm. 17: 69–73.
Vulto, A. G., Sharp, T., and Ungerstedt, U. (1985). Rapid post-mortal increase in extracellular concentration of dopamine in the rat as assessed by intra-cranial dialysis.Soc. Neuroci. Abstr. 11: 1207.
Weinberger, J., Nieves-Rosa, J., and Cohen, G. (1985). Nerve terminal damage in cerebral ischemia: Protective effect of alpha-methyl-para-tyrosine.Stroke 16: 864–870.
Wurtman, R. J., and Zervas, N. T. (1974). Monoamine neurotransmitters and the pathophysiology of stroke and central nervous system trauma.J. Neurosurg. 40: 34–36.
Yoshino, Y., and Elliot, K. A. C. (1970). Incorporation of carbon atoms from glucose into free amino acids in brain under normal and altered conditions.Can. J. Biochem. 48: 228–235.
Zervas, N. T., Hori, H., Negora, M., Wurtman, R. J., Larin, F., and Lavyne, M. H. (1974). Reduction in brain dopamine following experimental cerebral ischaemia.Nature 247: 283–284.
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Broderick, P.A., Gibson, G.E. Dopamine and serotonin in rat striatum duringin vivo hypoxic-hypoxia. Metabolic Brain Disease 4, 143–153 (1989). https://doi.org/10.1007/BF00999391
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DOI: https://doi.org/10.1007/BF00999391