Abstract
The physiologic function of proteasome remains unclear. Evidence suggests a role in degradation of ubiquitin-protein conjugates, MHC antigen presentation, and some specificity of substrate within certain cell types. To explore further the properties of proteasome we have examined its effect on a well defined structure, the myofibril. We find that despite its large size (20S) proteasome is able to degrade myofibrils and intact, permeabilized muscle fibrils. The proteins degraded showed some specificity because actin, myosin and desmin were degraded faster than α-actinin, troponin T and tropomyosin. Changes in ultrastructure were slow and included a general loss of structure with Z and I bands effected before the M band and costameres.
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Taylor, R.G., Tassy, C., Briand, M. et al. Proteolytic activity of proteasome on myofibrillar structures. Mol Biol Rep 21, 71–73 (1995). https://doi.org/10.1007/BF00990974
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DOI: https://doi.org/10.1007/BF00990974