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Effects of subchronic nicotine administration on central dopaminergic mechanisms in the rat

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Abstract

Nicotine was administered acutely and subchronically (14 days) to determine whether various synaptic mechanisms are selectively altered in the nigrostriatal and mesolimbic dopaminergic systems in the rat. When added to tissue preparations in vitro, nicotine had no effects on tyrosine hydroxylase, synaptosomal uptake of [3H]dopamine or binding of [3H]spiperone to D2 receptors in either system. However, acute treatment in vivo stimulated tyrosine hydroxylase activity in the nucleus accumbens. This effect was prevented by pretreatment with a nicotinic antagonist, suggesting that it was mediated by nicotinic receptors. Since subchronic exposure to nicotine had no effect on tyrosine hydroxylase, it appears that tolerance develops to this action. In vivo treatment with nicotine did not alter dopamine uptake or receptor binding. The results suggest that, in doses which result in moderate plasma levels, nicotine has selective stimulant actions on nerve terminals of the mesolimbic system.

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References

  1. Andersson, K., Fuxe, K., Eneroth, P., Gustafsson, J.-A., and Agnati, L. F. 1980. Mecamylamine induced blockade of nicotine induced inhibition of gonadotrophin and TSH secretion and of nicotine induced increase of catecholamine turnover in the rat hypothalamus. Acta Physiol. Scand. Suppl. 497:27–29.

    Google Scholar 

  2. Andersson, K., Fuxe, K., and Agnati, L. F. 1981. Effects of single injections of nicotine on the ascending dopamine pathways in the rat. Acta Physiol. Scand. 112:345–347.

    PubMed  Google Scholar 

  3. Annunziato, L., Leblanc, P., Kordon, C., and Weiner, R. L. 1980. Differences in the kinetics of dopamine uptake in synaptosome preparations of the median eminence relative to other dopaminergically inervated brain regions. Neuroendocrinology 31:316–320.

    PubMed  Google Scholar 

  4. Arqueros, L., Naquira, D., and Zunino, E. 1978. Nicotine-induced release of catecholamines from rat hippocampus and striatum. Biochem. Pharmacol. 27:2667–2674.

    Google Scholar 

  5. Benowitz, N. L., Kuyt, F. and Jacob, P. 1982. Circadian blood nicotine concentrations during cigarette smoking. Clin. Pharmacol. Ther. 32:758–764.

    PubMed  Google Scholar 

  6. Bustos, G., Simon, J., and Roth, R. H. 1980. Tyrosine hydroxylase regulation: apparent kinetic alterations following incubation of brain slices in a sodium-free medium. J. Neurochem. 35:47–57.

    PubMed  Google Scholar 

  7. Carr, L. A., Walters, D. E., and Meyer, D. C. 1985. Postnatal development in the rat following pre- or postnatal exposure to nicotine. Res. Commun. Subst. Abuse 6:151–164.

    Google Scholar 

  8. Feverabend, C., Ings, R. M. J. and Russell, M. A. H. 1985. Nicotine pharmacokinetics and its application to intake from smoking, Br. J. Clin. Pharmacol. 19:239–247.

    PubMed  Google Scholar 

  9. Fung, Y. K., and Lau, Y. S. 1986. Acute effect of nicotine on the striatal dopaminergic system in the rat. J. Pharm. Pharmacol. 38:920–922.

    PubMed  Google Scholar 

  10. Fuxe, K., Agnati, L. F., Eneroth, P., Gustafsson, J.-A., Hokfelt, T., Lofstrom, A., Skett, B., and Skett, P. 1977. The effect of nicotine on central catecholamine neurons and gonadotropin secretion. I. Studies in the male rat. Med. Biol. 55:148–157.

    PubMed  Google Scholar 

  11. Fuxe, K., Andersson, K., Harfstrand, A., and Agnati, L. F. 1986. Increases in dopamine utilization in certain limbic dopamine terminal populations after a short period of intermittent exposure of male rats to cigarettes smoke. J. Neural Transm. 67:15–29.

    PubMed  Google Scholar 

  12. Imperato, A., Mulas, A., and Di Chiara, G. 1986. Nicotine preferentially stimulates dopamine release in the limbic system of freely moving rats. European J. Pharmacol. 132:337–338.

    Google Scholar 

  13. Kirch, D. G., Gerhardt, G. A. Shelton, R. C., Freedman, R., and Wyatt, R. J. 1987. Effects of chronic nicotine administration on monoamine and monoamine metabolite concentrations in rat brain. Clin. Neuropharmacol. 10:376–383.

    PubMed  Google Scholar 

  14. Konig, J. F. R., and Klippel, R. A. 1963. The Rat Brain: A Stereotaxic Atlas of the Forebrain and Lower Parts of the Brain Stem, Robert E. Krieger, Huntington, N.Y.

    Google Scholar 

  15. Lichtensteiger, W., Felix, D., Hefti, F., and Schlumpf, M. 1979. Effects of nicotine on dopamine neurons of adult and prenatal mammals and of invertebrates. Pages 15–30,in Remond A., and Izard, C. (eds.), Electrophysiological Effects of Nicotine, Elsevier/North Holland Biomedical Press, Amsterdam.

    Google Scholar 

  16. Lowry, O. H., Roseborough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    PubMed  Google Scholar 

  17. Marks, M. J., Collins, A. C. 1985. Tolerance, cross-tolerance, and receptors after chronic nicotine or oxotremorine. Pharmacol. Biochem. Behav. 22:283–291.

    PubMed  Google Scholar 

  18. Marks, M. J., Stitzel, J. A., and Collins, A. C. 1985. Time course study of the effects of chronic nicotine infusion on drug response and brain receptors. J. Pharmacol. Exp. Ther. 235:619–628.

    PubMed  Google Scholar 

  19. Mishra, R. K., Wong, Y.-W., Varmuza, S. L. and Tuff, L. 1978. Chemical lesion and rug induced supersensitivity and subsensitivity of caudate dopamine receptors. Life Sci. 23:443–446.

    PubMed  Google Scholar 

  20. Pert, A., and Clarke, P. B. S. 1987. Nicotinic modulation of dopaminergic neurotransmission. Pages 169–189,in Martin, W. R., Van Loon, G. R., Iwamoto, E. T., and Davis, L. (eds.), Tobacco Smoking and nicotine, A Neurobiological Approach, Plenum Press, New York.

    Google Scholar 

  21. Rosecrans, J. A. 1987. Noncholinergic mechanisms involved in the behavioral and stimulus effects of nicotine, and relationships to the process of nicotine dependenc. Pages 125–139,in Martin, W. R., Van Loon, G. R., Iwamoto, E. T., and Davis, L. (eds.), Tobacco Smoking and Nicotine, A Neurobiological Apporach, Plenum Press, New York.

    Google Scholar 

  22. Rowell, P. P., Carr, L. A., and Garner, A. C. 1987. Stimulation of [3H]dopamine release by nicotine in rat nucleus accumbens. J. Neurochem. 49:1449–1454.

    PubMed  Google Scholar 

  23. Singer, G., Wallace, M., and Hall, R., 1982. Effects of dopaminergic nucleus accumbens lesions on the acquisition of schedule induced self injection of nicotine in the rat. Pharmacol. Biochem. Behav. 17:579–581.

    PubMed  Google Scholar 

  24. Tobias, H., Carr, L. A., and Voogt, J. L. 1981. Effect of estradiol benzoate and clomiphene on tyrosine hydroxylase activity and on luteinizing hormone and prolactin levels in ovariectomized rats. Life Sci. 29:711–716.

    PubMed  Google Scholar 

  25. Walters, D. E., and Carr, L. A., 1986. Changes in brain catecholamine mechanisms following perinatal exposure to marihuana. Pharmacol. Biochem. Behav. 25:763–768.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, University of Louisville, 40292, Louisville, Kentucky

    Laurence A. Carr, Peter P. Rowell & William M. Pierce Jr.

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  1. Laurence A. Carr
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  2. Peter P. Rowell
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  3. William M. Pierce Jr.
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Carr, L.A., Rowell, P.P. & Pierce, W.M. Effects of subchronic nicotine administration on central dopaminergic mechanisms in the rat. Neurochem Res 14, 511–515 (1989). https://doi.org/10.1007/BF00964911

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