Abstract
The autologous mixed lymphocyte response (AMLR) and the allogeneic mixed lymphocyte response were deficient in a subset of patients with newly diagnosed insulin-dependent diabetes mellitus (IDDM). Using a single set of HLA-identical twins, the cellular and molecular basis of deficient AMLR was investigated and appears to be due to a defect in both responder T cells and stimulator non-T cells. Interleukin-2 production was diminished in the patient but not in the healthy twin. Thein vitro addition of purified interleukin-2 enhanced the depressed AMLR in the diseased twin. This suggests that the deficient AMLR in IDDM may be in part due to a deficiency in the production of interleukin-2.
Similar content being viewed by others
References
DiMario U, Iavicoli M, Andreani D: Circulating immune complexes in diabetes. Diabetologia 19:89–92, 1980
Doberson MJ, Scharff JE, Ginsberg-Fallner F, Notkins AL: Cytotoxic autoantibodies to beta cells in the serum of patients with insulin-dependent diabetes mellitus. N Engl J Med 303:1493–1498, 1980
Gupta S, Fikrig SM, Khanna S, Orti E: Deficiency of suppressor T cells in insulin-dependent diabetes mellitus. An analysis with monoclonal antibodies. Immunol Lett 4: 289–294, 1982
Gupta S, Fikrig SM, Orti E: Autologous mixed lymphocyte reaction in man. VI. Deficiency of autologous mixed lymphocyte reaction in type 1 (insulin-dependent) diabetes mellitus. J Clin Lab Immunol 11:59–62, 1983
Gupta S, Fikrig SM, Khanna S, Orti E: Abnormal immunoregulatory function in patients with insulin-dependent diabetes mellitus. Human Lymph Diff 1:197–202, 1981
Riley WJ, MacLaren NK, Neufeld M: Adrenal autoantibodies and Addison's disease in insulin dependent diabetes mellitus. J Pediat 97:191–195, 1980
Selam JL, Clot J, Andary M, Mirouze J: Circulating lymphocyte subpopulations in juvenile insulin dependent diabetes mellitus. Diabetologia 16:35–40, 1979
Gupta S: Autologous mixed lymphocyte reaction in health and disease states in man. Vox Sang 44:265–288, 1983
Weksler ME, Kozak RW, Moody CE: The autologous mixed lymphocyte reaction. Adv Immunol 31:271–312, 1981
Chandy KG, Charles MA, Buckingham B, Waldeck N, Kershnar A, Gupta S: Deficiency of monoclonal antibody (Leu 7) defined NK cells in newly diagnosed insulin-dependent diabetes mellitus. Immunol Lett 8:89–91, 1984
Stern AS, Pan YC, Urdal DL, Mochizuki DY, DeChiana S, Blacher R, Wideman J, Gillis S: Purification to homogeneity and partial characterization of interleukin 2 from a human T cell leukemia. Proc Natl Acad Sci USA 81:871–874, 1982
Hansen JA, Martin PJ, Nowinski RC: Monoclonal antibodies identifying a novel T-cell antigen and Ia antigens of human lymphocytes. Immunogenetics 10:247–260, 1980
Gillis S, Ferm MW, Ou W, Smith KA: T-cell growth factor; Parameters of production and a qualitative microassay for activity. J Immunol 120:2027–2032, 1978
Steeg SP, Moore RN, Johnson HM, Oppenheim JJ: Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity. J Exp Med 156:1780–1783, 1982
Palacios R, Moller G: HLA-DR antigens render resting T cells sensitive to interleukin-2 and induce production of the growth factor in the autologous mixed lymphocyte reaction. Cell Immunol 63:143–153, 1981
Smith JB: Stimulation of autologous and allogeneic human T cells by B cells occurs through separate B cell antigen systems. Cell Immunol 36:203–209, 1978
Biddison WE: The role of the human major histocompatibility complex in cytotoxic T-cell responses to virus-infected cells. J Cin Immunol 2:1–9, 1982
Zinkernagel RM, Doherty PC: MHC-restricted cytotoxic T cells: Studies on the biologic role of polymorphic major transplantation antigens determining T-cell restriction, specificity, function, and responsiveness. Adv Immunol 27:52–177, 1979
Huber SA, MacPherson BR: Virus and insulin dependent diabetes mellitus. InImmunology of Clinical and Experimental Diabetes, S Gupta (ed). New York, Plenum Press, 1984, pp 295–321
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chandy, K.G., Charles, A.M., Kershnar, A. et al. Autologous mixed lymphocyte reaction in man: XV. Cellular and molecular basis of deficient autologous mixed lymphocyte response in insulin-dependent diabetes mellitus. J Clin Immunol 4, 424–428 (1984). https://doi.org/10.1007/BF00916571
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00916571