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Renal reabsorption of phosphate during development: tubular events

  • Proceedings of the Third International Workshop on Developmental Renal Physiology September 6–7, 1986 Tokyo, Japan
  • Original Article
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Abstract

Studies performed in our laboratory on the isolated perfused kidney of the guinea pig have demonstrated that the rate of Pi reabsorption is substantially greater in the newborn than in the adult, when appropriate corrections are being made either for differences in glomerular filtration rate (GFR) or in renal tubular mass. In order to determine the location of this enhanced reabsorption along the nephron, micropuncture experiments were performed on euvolemic, non-fasted guinea pigs 5–14 and 42–49 days of age, maintained on standard guinea-pig chow diet (0.76% Pi). Concomitant measurements of overall kidney function were also obtained. The results confirmed that fractional reabsorption of Pi (TRPi%) across the entire kidney was significantly higher (P<0.01) in the newborn (89.93±2.55%) than in the adult (78.25±2.89%) animals. The difference was also significant (P<0.05) when TRPi was expressed in mol/ml GFR (1.87±0.14 vs 1.53±0.12, respectively). At comparable locations along the proximal tubule (TF/Pin of 1.90±0.16 in the newborn, and 1.79±0.15 in the adult,P>0.70), the fraction of the filtered load of Pi reabsorbed was significantly higher (P<0.001) in the immature (76.66±2.74%) than in the mature (67.21±2.74%) guinea pigs. Estimates based on the differences between proximal Pi reabsorption and the urinary excretion of Pi indicate that the reabsorption of Pi in tubular segments located beyond the proximal tubule is also enhanced in the newborn when compared with the adult (15.62±2.11% vs 10.51±1.83%, respectively,P<0.05). Alternatively, this finding may be interpreted to represent a higher fractional reabsorption of Pi by deep nephrons.

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References

  1. Dean RFA, McCance RA (1984) Phosphate clearance in infants and adults. J Physiol (Lond) 107: 182–186

    Google Scholar 

  2. Richmond JB, Kravitz H, Segar W, Waisman HA (1951) Renal clearance of endogenous phosphate in infants and children. Proc Soc Exp Biol Med 77: 83–87

    Google Scholar 

  3. Brodehl J, Gellissen K, Weber HP (1982) Postnatal development of tubular phosphate reabsorption. Clin Nephrol 17: 163–171

    Google Scholar 

  4. McCrory WW, Forman CW, McNamara H, Barnett HL (1952) Renal excretion of inorganic phosphate in newborn infants. J Clin Invest 31: 357–365

    Google Scholar 

  5. Johnson V, Spitzer A (1981) Renal reabsorption of Pi during development: whole kidney events. Am J Physiol 251 [20]: F251–F256

    Google Scholar 

  6. Caverzasio J, Bonjour JP, Fleisch H (1982) Tubular handling of Pi in young growing and adult rats. Am J Physiol 242 [11]: F705–F710

    Google Scholar 

  7. Russo JC, Nash MA (1980) Renal response to alterations in dietary phosphate in the young beagle. Biol Neonate 38: 1–10

    Google Scholar 

  8. Evan AP, Vincent GH, Schwartz GJ (1983) Development of solute transport in rabbit proximal tubule. II. Morphologic segmentation. Am J Physiol 245 [14]: F391–F407

    Google Scholar 

  9. Hay DA, Evan AP (1979) Maturation of the proximal tubule in the puppy kidney: a comparison to the adult. Anat Rec 195: 273–299

    Google Scholar 

  10. Staum BB, Hamburger RJ, Goldberg M (1972) Tracer microinjection study of renal tubular phosphate reabsorption in the rat. J Clin Invest 51: 2271–2276

    Google Scholar 

  11. Ullrich KJ, Rumrich G, Kloss S (1977) Phosphate transport in the proximal convolution of the rat kidney. I. Tubular heterogeneity; effect of parathyroid hormone in acute and chronic parathyroidectomized animals, and effect of phosphate diet. Pflügers Arch 372: 269–274

    Google Scholar 

  12. Aperia A, Larsson L, Zetterstrom R (1981) Hormonal induction of Na+−K+-ATPase in developing proximal tubular cells. Am J Physiol 241 [10]: F356–F361

    Google Scholar 

  13. Schwartz GJ, Evan AP (1984) Development of solute transport in rabbit proximal tubule. III. Na−K-ATPase activity. Am J Physiol 246 [15]: F845–F852

    Google Scholar 

  14. Aperia A, Elinder G (1981) Distal tubular sodium reabsorption in developing rat kidney. Am J Physiol 240 [9]: F487–F491

    Google Scholar 

  15. Schoeneman MJ, Spitzer A (1980) The effect of intravascular volume expansion on proximal tubular reabsorption during development. Proc Soc Exp Biol Med 165: 315–322

    Google Scholar 

  16. Spitzer A (1982) The role of the kidney in sodium homeostasis during development. Kidney Int 21: 539–545

    Google Scholar 

  17. Root A, Gruskin A, Reber RM, Stopa A, Duckett J (1974) Serum concentration of parathyroid hormone in infants, children and adolescents. J Pediatr 85: 329–336

    Google Scholar 

  18. Tsang RC, Chen IW, Friedman MA, Chen I (1973) Neonatal parathyroid function. Role of gestational age and post-natal age. J Pediatr 33: 728–738

    Google Scholar 

  19. Gengler WR, Forte LR (1972) Neonatal development of rat kidney adenyl cyclase and phosphodiesterase. Biochim Biophys Acta 279: 367–373

    Google Scholar 

  20. Linarelli LG, Bobik J, Bobik C (1972) Newborn urinary cyclic AMP and developmental renal responsiveness to parathroid hormone. Pediatrics 50: 14–23

    Google Scholar 

  21. Beck LH, Goldberg M (1974) Mechanism of blunted phosphaturia in saline loaded thyroparathyroidectomized dogs. Kidney Int 6: 18–23

    Google Scholar 

  22. Beck LH, Goldberg M (1973) Effects of acetazolamide and parathyroidectomy on renal transport of sodium, calcium and phosphate. Am J Physiol 224 [5]: 1136–1142

    Google Scholar 

  23. Spitzer A, Brandis M (1974) Functional and morphologic maturation of the superficial nephrons. Relationship to total renal function. J Clin Invest 53: 279–287

    Google Scholar 

  24. Fiske HC, Subarow Y (1925) The colorimetric determination of phosphorous. J Biol Chem 66: 375–400

    Google Scholar 

  25. Lechene C (1970) The use of the electron microprobe to analyze every minute amounts of liquid samples. Proc Natl Conf Electron Probe Anal 5: 32A-32C

    Google Scholar 

  26. Vurek GG, Pegram SE (1966) Fluorometric method for the determination of nanogram quantities of inulin. Anal Biochem 16: 409–419

    Google Scholar 

  27. Strickler JC, Thompson DD, Klose RM, Giebisch G (1964) Micropuncture study of inorganic phosphate excretion in the rat. J Clin Invest 43: 1596–1607

    Google Scholar 

  28. Amiel C, Kuntziger HE, Richet G (1970) Micropuncture study of handling of phosphate by proximal and distal nephron in normal and parathyroidectomized rat. Evidence for distal reabsorption. Pflügers Arch 317: 93–109

    Google Scholar 

  29. Baumann K, de Rouffignac C, Roinel N, Rumrich G, Ullrich KJ (1975) Renal phosphate transport: inhomogeneity of local proximal transport rates and sodium dependence. Pflügers Arch 356: 287–297

    Google Scholar 

  30. Dennis VW, Bello-Reuss E, Robinson RR (1977) Response of phosphate transport to parathyroid hormone in segment of rabbit nephron. Am J Physiol 233: [2] F29–F38

    Google Scholar 

  31. Pastoriza-Munoz E, Mishler DR, Lechene C (1983) Effect of phosphate deprivation on phosphate reabsorption in rat nephron: role of PTH. Am J Physiol 244 [13]: F140–F149

    Google Scholar 

  32. Larsson L (1975) The ultrastructure of the developing proximal tubule in the rat kidney. J Ultrastruct Res 51: 119–139

    Google Scholar 

  33. Friis C (1980) Postnatal development of the pig kidney: ultrastructure of the glomerulus and the proximal tubule. J Anat 130: 513–520

    Google Scholar 

  34. Oliver J (1968) Structural status of the kidney at birth. In: Nephrons and kidneys. A quantitative study of development and evolutionary mammalian renal architectonics. Harper and Row, New York, pp 4–116

    Google Scholar 

  35. Goldfarb S (1980) Juxtamedullary and superficial nephrons phosphate reabsorption in the cat. Am J Physiol 239 [8]: F336–F342

    Google Scholar 

  36. Knox FG, Haas JA, Berndt T, Marchand GR, Youngberg SP (1977) Phosphate transport in superficial and deep nephrons in phosphate loaded rats. Am J Physiol 233 [2]: F150–F153

    Google Scholar 

  37. Haramati A, Haas JA, Knox FG (1983) Adaptation of deep and superficial nephrons to changes in dietary phosphate intake. Am J Physiol 244 [13]: F265–F269

    Google Scholar 

  38. Haas JA, Berndt T, Knox FG (1978) Nephron heterogeneity of phosphate reabsorption. Am J Physiol 234 [3]: F287–F290

    Google Scholar 

  39. McKeown JW, Brazy PC, Dennis VW (1979) Intrarenal heterogeneity for fluid, phosphate, and glucose absorption in the rabbit. Am J Physiol 237 [6]: F312–F318

    Google Scholar 

  40. Spitzer A, Kaskel FJ, Feld LG, Trachtman H, Johnson V, Choi Y, Kumar AM (1983) Renal regulation of phosphate homeostasis during growth. Semin Nephrol 3: 87–93

    Google Scholar 

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Authors and Affiliations

  1. Division of Nephrology, Department of Pediatrics, Albert Einstein College of Medicine, 10461, Bronx, NY, USA

    Frederick J. Kaskel, Adarsh M. Kumar, Leonard G. Feld & Adrian Spitzer

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  1. Frederick J. Kaskel
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  2. Adarsh M. Kumar
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  3. Leonard G. Feld
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  4. Adrian Spitzer
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Kaskel, F.J., Kumar, A.M., Feld, L.G. et al. Renal reabsorption of phosphate during development: tubular events. Pediatr Nephrol 2, 129–134 (1988). https://doi.org/10.1007/BF00870393

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