Abstract
In Henoch-Schönlein purpura nephritis (HSPN), glomeruli may develop cellular “crescents” composed of infiltrating monocytes and proliferating renal epithelia. In this study, we demonstrate that peripheral human monocytes can release an epidermal growth factor (EGF)-like substance detectable by a radioreceptor assay, which recognizes both EGF and transforming growth factor-alpha (TGF-alpha), but not with a radioimmunoassay, which recognizes only EGF. Furthermore, we report that urine from pediatric patients during the acute phase of HSPN contains a similar EGF-like species in addition to the endogenous EGF which is normally present. The EGF-like material was not present in urine from nine healthy children or from six children with acute post-streptococcal glomerulonephritis. The extent of crescent formation in our patients is uncertain, since renal biopsy was performed in only one case. However, we speculate that the urinary material resembling TGF-alpha which appears during the acute phase of HSPN may derive from monocytes infiltrating the kidney.
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References
Hancock WW, Atkins RC (1984) Cellular composition of crescents in human rapidly progressive glomerulonephritis identified using monoclonal antibodies. Am J Nephrol 4:177–181
Golds EE, Santer V, Killackey J, Roughly PJ (1983) Mononuclear cell factors stimulate the concomitant secretion of distinct latent proteoglycan, gelatin and collagen degrading enzymes from human skin fibroblasts and synovial cells. J Rheumatol 10:861–871
Magill AB, Wadsworth MB (1982) Monocyte involvement in glomerular crescents. Lab Invest 47:160–166
Haycock GB (1988) The treatment of glomerulonephritis in children. Pediatr Nephrol 2:247–255
Nolasco FEB, Cameron JS, Hartley B, Coelho A, Hildreth G, Reuben R (1987) Intraglomerular T cells and monocytes in nephritis: study with monoclonal antibodies. Kidney Int 31:1160–1166
Nathan CF (1987) Secretory products of macrophages. J Clin Invest 79:319–326
Madtes DK, Raines EW, Sakariassen KS, Assoian RK, Sporn MB, Bell GI, Ross R (1988) Induction on transforming growth factor-alpha in activated human alveolar macrophages. Cell 53:285–293
Carpenter G (1987) Receptors for epidermal growth factor and other polypeptide mitogens. Annu Rev Biochem 56: 881–914
Goodyer PR, Kachra Z, Bell C, Rozen R (1988) Renal tubular cells are targets for epidermal growth factor. Am J Physiol 255:F1191-F1196
Lai WH, Guyda HJ (1984) Characterization and regulation of epidermal growth factor receptors in human placental cell cultures. J Clin Endocrinol Metab 58:344–352
Mattila A-L, Perheentupa J, Pesonen K, Viinkka L (1985) Epidermal growth factor in human urine from birth to puberty. J Clin Endocrinol Metab 61:997–1000
Salido EC, Barajas L, Lechago J, Laborde NP, Fisher DA (1986) Immunocytochemical localization of epidermal growth factor in mouse kidney. J Histochem Cytochem 34:1155–1160
Mattila A-L, Pasternack A, Viinkka L, Perheentupa J (1986) Subnormal concentrations of urinary epidermal growth factor in patients with kidney disease. J Clin Endocrinol Metab 62:1180–1183
Goodyer PR, Fata J, Kaplan BS (1987) Potential role for EGF in proliferative glomerulonephritis. Xth Int Congr Nephrol (London) A328
Cybulsky AV, Bonventre JV, Quigg RJ, (1988) Extracellular matrix (ECM) regulates proliferation and phospholipid turnover in glomerular epithelial cells (GEC). Am Soc Nephrol 21st Meeting 22A
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Goodyer, P.R., Fata, J. & Goodyer, C.G. Excretion of epidermal growth factor-like material in acute Henoch-Schönlein purpura nephritis. Pediatr Nephrol 4, 101–104 (1990). https://doi.org/10.1007/BF00858818
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DOI: https://doi.org/10.1007/BF00858818