Summary
The distribution of various tissue antigens was studied in mucoepidermoid carcinomas (n=74) and acinic cell carcinomas (n=38) by means of immunocytochemistry. Mucoepidermoid carcinomas were generally positive for cytokeratin and showed double expression for cytokeratin and vimentin in 31.1% and triple expression for cytokeratin, vimentin and GFAP in 24.1%. CEA was studied using new monoclonal antibodies which distinguish between epitopes that are present on CEA alone and those which are present on nonspecific cross reacting antigens as well. The monospecific CEA antibody was completely negative in mucoepidermoid carcinomas, while nonspecific cross reacting antigens (NCAs) were positive in mucoepidermoid carcinomas to a varying degree. Alpha 1-antichymotrypsin, a marker formerly thought to be specific for tissues for histiocytic origin, was positive in 85.1% of mucoepidermoid carcinomas. Twenty three percent of mucoepidermoid carcinomas showed focal infiltration by S-100 positive dendritic stromal cells, tumour cell being negative. Leu-M 1 antigen was positive in 58.1% of mucoepidermoid carcinomas. Acinic cell carcinomas were generally positive for cytokeratin and in single cases showed double expression for cytokeratin and vimentin and triple expression for cytokeratin, vimentin and GFAP. Monospecific CEA antibody positivity could be demonstrated in 24.2% of acinic cell carcinoma, while nonspecific cross reacting antigens (NCAs) were positive in acinic cell carcinomas to a varying degree. Alpha 1-antichymotrypsin was positive in 97.4% of acinic cell carcinomas. 2.5% of acinic cell carcinomas showed focal infiltration by S-100 positive dendritic stromal cells, 2.5% of acinic cell carcinomas were positive for S-100 protein with no dendritic stromal cells present. Leu-M 1 antigen was positive in 86.8% of acinic cell carcinomas. For S-100 protein and Leu-M 1, no correlation with the clinical course, as reported previously for other tumours, could be observed.
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References
Achtstätter T, Moll R,Anderson A, Kuhn C, Pitz S, Schwechheimer K, Franke WW (1986) Expression of glial filament protein (GFP) in nerve sheaths and non-neural cells reexamined using monoclonal antibodies, with special emphasis on the coexpression of GFP and cytokeratins in epithelial cells of human salivary gland and pleomorphic adenoma. Differentiation 31:206–227
Altmannsberger M, Weber K, Hölscher A, Schauer A, Osborn M (1982) Antibodies to intermediate filaments as diagnostic tools. Human gastrointestinal carcinomas express prekeratin. Lab Invest 46:520–526
Aroni K, Kittas C, Papadimitriou CS, Papacharalampous NX (1984) An immunocytochemical study of the distribution of lysozyme, a 1-antitrypsin and a 1-antichymotrypsin in the normal and pathological gall bladder. Virchows Arch [A] 403:281–285
Bätge B, Bosslet K, Sedlacek HH, Kern HF, Klöppel G (1986) Monoclonal antibodies against CEA-related components discriminate between pancreatic duct type carcinomas and nonneoplastic duct lesions as well as nonduct type neoplasias. Virchows Arch [A] 408:361–374
Bosslet K, Lüben G, Schwarz A, Hundt E, Harthus HP, Seiler FR, Muhrer C, Klöppel G, Kayser K, Sedlacek HH (1985) Immunohistochemical localization and molecular characteristics of three monoclonal antibody defined epitopes detectable on carcinoembryonic (CEA). Int J Cancer 36:75–84
Caselitz J (1987) Das pleomorphe Adenom der Speicheldrüsen. Histogenese, zelluläre Differenzierung, Tumormarker. Fischer, Stuttgart
Caselitz J, Jaup T, Seifert G (1981a) Immunohistochemical detection of carcinoembryonic antigen (CEA) in parotid gland carcinomas. Analysis of 52 cases. Virchows Arch [A] 394:49–60
Caselitz J, Seifert G, Jaup T (1981b) Presence of carcinoembryonic antigen (CEA) in the normal and inflamed human parotid gland. An immunohistochemical study of 31 cases. J Cancer Res Clin Oncol 100:205–211
Caselitz J, Osborn M, Wustrow J, Seifert G, Weber K (1982) The expression of different intermediate-sized filments in human salivary glands and their tumours. Pathol Res Pract 175:266–278
Caselitz J, Becker J, Seifert G, Weber K, Osborn M (1984) Coexpression of keratin and vimentin filaments in adenoid cystic carcinomas of salivary glands. Virchows Arch [A] 403:337–344
Denk H, Tappeiner G, Eckerstorfer R, Holzner JH (1972) Carcinoembryonic antigen (CEA) in gastrointestinal and extra-gastrointestinal tumors and its relationship to tumor cell differentiation. Int J Cancer 10:262–272
Furukawa T, Watanabe S, Kodama T, Sato Y, Shimosato Y, Suemasu K (1985) T-zone histiocytes in adenocarcinoma of the lung in relation to postoperative prognosis. Cancer 56:2651–2656
Hara K, Ito M, Takeuchi J, Iijima S, Endo T, Hidaka H (1983) Distribution of S-100 b protein in normal salivary glands and salivary gland tumors. Virchows Arch [A] 401:237–249
Heerde P van Feltkamp CA, Hart AAM, Somers R (1984) Malignant histiocytosis and related tumours. A clinicopathological study of 42 cases using cytological, histochemical and ultrastructural parameters. Haematol Oncol 2:13–32
Hsu SM, Jaffe ES (1984) Leu-M 1 and peanut agglutinin stain the neoplastic cells of Hodgkins's disease. Am J Clin Pathol 82:29–32
Kittas C, Aroni K, Kotsis L, Papadimitriou CS (1982a) Distribution of lysozyme, alpha 1-antichymotrypsin and alpha 1-antitrypsin in adenocarcinomas of the stomach and large intestine. Virchows Arch [A] 398:139–147
Kittas C, Aroni K, Matani A, Papadimitriou CS (1982b) Immunocytochemical demonstration of alpha 1-antitrypsin and alpha 1-antichymotrypsin in human gastrointestinal tract. Hepato-Gastroenterology 29:275–277
Kornstein MJ, Bonner H, Gee B, Cohen R, Brooks JJ (1986) Leu-M 1 and S 100 in Hodgkin's disease and non-Hodgkin's lymphomas. Am J Clin Pathol 85:433–437
Leader M, Patel J, Collins M, Henry K (1987) Anti-alpha 1-antichymotrypsin staining of 194 sarcomas, 38 carcinomas, and 17 malignant melanomas. Its lack of specifity as a tumour maker. Am J Surg Pathol 11:133–139
Mori M, Tsukitani K, Ninomiya T, Okada Y (1987) Various expressions of modified myoepithelial cells in salivary pleomorphic adenoma. Pathol Res Pract 182:632–646
Motoi M, Stein H, Lennert K (1980) Demonstration of lysozyme, alpha 1-antichymotrypsin, alpha 1-antitrypsin albumin and transferrin with the immunoperoxidase method in lymph node cells. Virchows Arch [B] 35:73–82
Murase N, Kobayashi K, Mitani H, Mori M (1985) Immunohistochemical localization of alpha 1-antitrypsin and alpha 1-antichymotrypsin in salivary pleomorphic adenomas. Virchows Arch [A] 408:107–116
Nakajima T, Kameya T, Watanabe S, Hirota T, Sato Y, Shimosato Y (1982) An immunoperoxidase study of S-100 protein distribution in normal and neoplastic tissues. Am J Surg Pathol 6:715–727
Nakazato Y, Ishida Y, Takahashi K, Suzuki K (1985) Immunohistochemical distribution of S-100 protein and glial fibrillary acidic protein in normal and neoplastic salivary glands. Virchows Arch [A] 405:299–310
Nomori H, Watanabe S, Nakajima T, Shimosato Y, Kameya T (1986) Histiocytes in nasopharyngeal carcinoma in relation to prognosis. Cancer 57:100–105
Osborn M, Weber K (1983) Biology of disease. Tumor diagnosis by intermediate filament typing: A novel tool for surgical pathology. Lab Invest 48:372–394
Permanetter W, Meister P (1984) Distribution of lysozyme (muramidase) and alpha 1-antichymotrypsin in normal and neoplastic epithelial tissue. A survey. Acta histochem 74:173–179
Pruss RM, Mirsky R, Raff MC, Thorpe R, Dowding AJ, Anderton BH (1981) All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody. Cell 27:419–428
Ramaekers FCS, Haag D, Kant A, Moeskers O, Jap PHK, Vooijs GP (1983) Coexpression of keratin- and vimentin-type intermediate filaments in human metastatic carcinoma cells. Proc Natl Acad Sci 80:2618–2622
Regezi JA, Lloyd R, Zarbo R J, McClatchey KD (1985) Minor salivary gland tumors. A histologic and immunohistochemical study. Cancer 55:108–115
Schröder S, Klöppel G (1987) Carcinoembryonic antigen and nonspecific cross-reacting antigen in thyroid cancer. An immunocytochemical study using polyclonal and monoclonal antibodies. Am J Surg Pathol 11:100–108
Schröder S, Schwarz W, Rehpenning W, Löning T, Böcker W (1987) Prognostic significance of Leu-M 1 immunostaining in papillary carcinomas of the thyroid gland. Virchows Arch [A] 411:435–439
Schröder S, Schwarz W, Rehpenning W, Löning T, Böcker W (1988a) Dendritic/Langerhans cells and prognosis in patients with papillary thyroid carcinomas. Immunocytochemical study of 106 thyroid neoplasms correlated to follow-up data. Am J Clin Pathol 89:295–300
Schröder S, Schwarz W, Rehpenning W, Dralle H, Bay V, Böcker W (1988b) Leu-M 1 immunoreactivity and prognosis in medullary carcinomas of the thyroid gland. J Cancer Res Clin Oncol 114:291–296
Sehested M, Barfoed C, Krogdahl A, Bretlau P (1985) Immunohistochemical investigation of lysozyme, lactoferrin, alpha 1-antitrypsin, alpha 1-antichymotrypsin and ferritin in parotid gland tumors. J Oral Pathol 14:459–465
Seifert G, Caselitz J (1985) Markers of oral and salivary gland tumors. Cancer Detect Prevent 8:23–34
Sheibani K, Battifora H, Burke JS, Rappaport H (1986) Leu-M 1 antigen in human neoplasms. An immunohistologic study of 400 cases. Amer J Surg Pathol 10:227–236
Sumitomo S, Kumasa S, Mitani H, Mori M (1987) Comparison of CEA distribution in lesions and tumors of salivary glands as determined with monoclonal and polyclonal antibodies. Virchows Arch Cell Pathol 53:133–139
Takahashi H, Tsuda N, Tezuka F, Okaba H (1988) Difference of immunohistochemical reactions in epithelial cells of adenolymphoma. J Oral Pathol 17:287–292
Terry WD, Henkart PA, Coligan JE, Todd CW (1974) Carcinoembryonic antigen: characterization and clinical application. Transplant Rev. 20:100–129
Toto PD, Hsu DJ (1985) Product definition of pleomorphic adenoma of minor salivary glands. J Oral Pathol 14:818–832
Tsujitani S, Furukawa T, Tamada R, Okamura T, Yasumoto K, Sugimachi K (1987) Langerhans cells and prognosis in patients with gastric carcinoma. Cancer 59:501–505
Tsukitani K, Nakai M, Tatemoto Y, Hikosaka N, Mori M (1985) Histochemical studies of obstructive adenitis in human submandibular salivary glands. I. Immunohistochemical demonstration of lactoferrin, lysozyme and carcinoembryonic antigen. J Oral Pathol 14:631–638
Zarbo R, Regezi JA, Batsakis JG (1986) S-100 protein in salivary gland tumors: an immunohistochemical study of 129 cases. Head Neck Surg 8:268–275
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Supported by the Deutsche Forschungsgemeinschaft (DFG), the Hamburger Landesverband für Krebsbekämpfung und Krebsforschung, and the Hamburger Stiftung zur Förderung der Krebsbekämpfung
Prof. Dr. Dres. h.c. W. Doerr on the occasion of his 75th birthday
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Hamper, K., Schmitz-Wätjen, W., Mausch, HE. et al. Multiple expression of tissue markers in mucoepidermoid carcinomas and acinic cell carcinomas of the salivary glands. Vichows Archiv A Pathol Anat 414, 407–413 (1989). https://doi.org/10.1007/BF00718624
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DOI: https://doi.org/10.1007/BF00718624