Summary
Chandipura virus was inoculated intraperitoneally into 9-day old mice. Rising viral titers were detected in blood, skeletal muscle and viscera, beginning 3 to 6 h post-inoculation. Significant quantities of virus were initially noted in the brain at 24 h, and titers in that region rose precipitously during the succeeding 72 h. Detectable clinical signs, including neurologic dysfunctions began 48 h post-inoculation. Death was common at 72 and 96 h. The most significant and consistently observed lesions were in the central nervous system and included necrosis of neurons and ependymal cells.
Similar content being viewed by others
References
Bhatt, P. N., Rodrigues, F. M.: Chandipura: a new arbovirus isolated in India from patients with febrile illness. Indian J. med. Res.55, 1295–1305 (1967).
Bruno-Lobo, M., Peralta, P. H., Bruno-Lobo, G. G., DePaola, D.: Pathogenesis of vesicular stomatitis virus infection in the infant hamster and mouse. An. Microbiol. (Rio de J.)15, 53–68 (1968).
Dhandha, V., Rodrigues, F. M., Ghosh, S. N.: Isolation of Chandipura virus from sandflies in Aurangabad. Indian J. med. Res.58, 179–180 (1970).
Falke, D., Rowe, W. P.: Die Erkrankung der Maus durch das Virus der Stomatitis vesicularis. I. Die Ausbreitung des Virus in Abhängigkeit vom Alter der Maus. Arch. ges. Virusforsch.17, 549–559 (1965a).
Falke, D., Rowe, W. P.: Die Erkrankung der Maus durch das Virus der Stomatitis vesicularis. II. Die Pathologie der Organläsionen und der Befall das zentralen und peripheren Nerven-systems. Arch. ges. Virusforsch.17, 560–576 (1965b).
Johnson, R. T., Johnson, K. P.: Hydrocephalus following viral infection: the pathology of aqueductal stenosis developing after experimental mumps virus infection. J Neuropath. exp. Neurol.27, 591–600 (1968).
Johnson, R. T., Johnson, K. P.: Hydrocephalus as a sequela of experimental myxovirus infections. Exp. molec. Path.10, 68–80 (1969).
Kemp, G. E.: personal communication (1970).
Kilham, L., Margolis, G.: Hydrocephalus in hamsters, ferrets, rats and mice following inoculations with reovirus type I. I. Virologic studies. Lab. Invest.21, 183–188 (1969).
Margolis, G., Kilham, L.: Virus-induced cerebellar hypoplasia. Res. Publ. Ass. nerv. ment. Dis.44, 113–146 (1968).
Margolis, G., Kilham, L.: Hydrocephalus in hamsters, ferrets, rats and mice following inoculations with reovirus type I. II. Pathologic studies. Lab. Invest.21, 189–198 (1969).
Margolis, G., Kilham, L., Johnson, R. H.: The parvoviruses and replicating cells: insights into the pathogenesis of cerebellar hypoplasia. Progr. Neuropath.1, 168–201 (1971).
Mims, C. A.: Intracerebral injections and the growth of viruses in the mouse brain. Brit. J. exp. Path.41, 52–59 (1960a).
Mims, C. A.: An analysis of the toxicity of influenza virus. I. Intracerebral toxicity. Brit. J. exp. Path.41, 586–592 (1960b).
Murphy, F. A., Harrison, A. K., Whitfield, S. G.: The infection of cells by bullet-shaped viruses. 27th Ann. Proc. Electron Microscopic Soc. Am. (1969).
Murphy, F. A., Shope, R. E.: Comparison of the characteristics of vertebrate viruses-bullet shaped & bacilliform viruses. Int. Virol.2, 261–262 (1971) (Proc. 2nd int. Congr. Virol., Budapest).
Murphy, F. A., Shope, R. E., Metselaar, D., Simpson, D. I. H.: Characterization of Mount Elgon bat virus, a new member of the rhabdovirus group. Virology40, 288–297 (1970).
Reed, L. J., Muench, H.: A simple method of estimating fifty percent endpoints. Amer. J. Hyg.27, 493–497 (1938).
Rhim, J. S., Schell, K., Creasy, B., Case, W.: Biological characteristics and viral susceptibility of an African green monkey kidney cell line (Vero). Proc. Soc. exp. Biol. (N.Y.)132, 670–678 (1969).
Sabin, A. B., Olitsky, P. K.: Influence of host factors on neuroinvasiveness of vesicular stomatitis virus. III. Effect of age and pathway of infection on the character and localization of lesions in the central nervous system. J. exp. Med.67, 201–228 (1938).
Author information
Authors and Affiliations
Additional information
Supported by U.S. Public Health Service Grants PHS-FR-05358-08 and PHS 2 PO 6 RR 00393.
Rights and permissions
About this article
Cite this article
Jortner, B.S., Bhatt, P.N. & Solitare, G.B. Experimental Chandipura virus infection in mice. Acta Neuropathol 23, 320–325 (1973). https://doi.org/10.1007/BF00687461
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00687461