Summary
We demonstrate that the steroid hormone receptor-positive breast carcinoma cell lines T-47D and MCF-7 can be induced by androgens, progestins, mineralocorticosteroids, glucocorticosteroids, and antiestrogens, to produce prostate specific antigen (PSA). Estrogens failed to induce such stimulation in both cell lines and, in addition, were able to block the induction by androgens in the cell line T-47D. These data support and extend our previous report on PSA production by breast tumors and describe anin vitro system which can be used to study the phenomenon for possible application in prognosis and design of new therapy.
References
Oesterling JE: Prostate specific antigen: a critical assessment of the most useful tumor marker for adenocarcinoma of the prostate. J Urol 45: 907–923, 1991
Armbruster DA: Prostate-specific antigen: biochemistry, analytical methods and clinical application. Clin Chem 39: 181–195, 1993
Hudson MA, Bahnson RR, Catalona WJ: Clinical use of prostate specific antigen in patients with prostate cancer. J Urol 142: 1011–1017, 1989
Van Krieken JHJM: Prostate marker immunoreactivity in salivary gland neoplasms. A rare pitfall in immunohistochemistry. Am J Surg Pathol 17: 410–414, 1993
Papotti M, Paties C, Peveri V, Moscuzza L, Bussolati G: Immunocytochemical detection of prostate-specific antigen (PSA) in skin adnexal and breast tissues and tumors. Bas Appl Histochem 33: 25–29, 1989
Diamandis EP, Yu H, Sutherland DJA: Detection of immunoreactive prostate-specific antigen in breast tumors. Breast Cancer Res Treat (in press), 1994
Read LD, Snider CE, Miller JS, Greene GL, Katzenellenbogen BS: Ligand-modulated regulation of progesterone receptor messenger ribonucleic acid and protein in human breast cancer cell lines. Mol Endocrinol 2: 263–271, 1988
Read LD, Greene GL, Katzenellenbogen BS: Regulation of estrogen receptor messenger ribonucleic acid and protein levels in human breast cancer cell lines by sex steroid hormones, their antagonists, and growth factors. Mol Endocrinol 3: 295–304, 1989
Hall RE, Lee CSL, Alexander IE, Shine J, Clarke CL, Sutherland RL: Steroid hormone receptor gene expression in human breast cancer cells: inverse relationship between estrogen and glucocorticoid receptor messenger RNA levels. Int J Cancer 46: 1081–1087, 1990
May FEB, Westley BR: Identification and characterization of estrogen-regulated RNAs in human breast cancer cells. J Biol Chem 263: 12901–12908, 1988
Yu H, Diamandis EP: Ultrasensitive time-resolved immunofluorometric assay of prostate-specific antigen in serum and preliminary clinical studies. Clin Chem 39: 2108–2114, 1993
Beato M: Gene regulation by steroid hormones. Cell 56: 335–344, 1989
Lea A, Kvinnsland S, Thorsen T: Improved measurement of androgen receptors in human breast cancer. Cancer Res 49: 7162–7167, 1989
McGinnis MY, Davies PG, Meaney MJ, Singer M, McEwen BS:In vitro measurement of cytosol and cell nuclear androgen receptors in male rat brain and pituitary. Brain Res 275: 75–82, 1983
Yu H, Diamandis EP, Levesque MA, Sismondi P, Zola P, Katsaros D: Ectopic production of prostate specific antigen by a breast tumor metastatic to the ovary. J Clin Lab Analysis (in press), 1994
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yu, H., Diamandis, E.P., Zarghami, N. et al. Induction of prostate specific antigen production by steroids and tamoxifen in breast cancer cell lines. Breast Cancer Res Tr 32, 291–300 (1994). https://doi.org/10.1007/BF00666006
Issue Date:
DOI: https://doi.org/10.1007/BF00666006