Abstract
The effects of laparoscopic colectomy on immune function require further study. Because the rat has been widely used to investigate the immune system we studied the feasibility of laparoscopic sutured colon anastomoses in a rat model. We used a low-pressure CO2 pneumoperitoneum, a 2.7-mm 30° wide-angle arthroscope and video system, and microotologic forceps and scissors to perform two-handed dissection and suturing of the rat colon. The procedures included laparoscopic division of the colon and colostomy (group A,n=3), laparoscopic closure of a colotomy (group B,n=4), and complete laparoscopic division and reanastomosis of the colon (group C,n=4). One anesthetic death occurred in group C. Necropsy performed 1–15 days postoperatively revealed no anastomotic leaks and two anastomotic strictures. Laparoscopic sutured colon anastomosis in the rat is feasible and may provide a new model for the physiologic study of laparoscopic colon surgery.
Similar content being viewed by others
References
Berguer R, Gutt CN, Stiegmann GV (1993) Laparoscopic surgery in the rat: description of a new technique. Surg Endosc 7: 345–347
Berkenbosch F, Wolvers DA, Derijk R (1991) Neuroendocrine and immunological mechanisms in stress-induced immunomodulation. J Steroid Biochem Mol Biol 40: 639–647
Cooper GM, Scoggins AM, Ward ID, Murphy D (1982) Laparoscopy: a stressful procedure. Anaesthesia 37: 266–269
Falk PM, Beart R Jr, Wexner SD, Thorson AG, Jagelman DG, Lavery IC, Johansen OB, Fitzgibbons R Jr (1993) Laparoscopic colectomy: a critical appraisal. Dis Colon Rectum 36: 28–34.
Filmar S, Gomel V, McComb PF (1987) Operative laparoscopy versus open abdominal surgery: a comparative study on post-operative adhesion formation in the rat model. Fertil Steril 48: 486–489
Flecknell, PA (1987) Laboratory animal anesthesia. Academic Press, San Diego
Garcia-Caballero M, Vara-Thorbeck C (1993) The evolution of postoperative ileus after laparoscopic cholecystectomy: a comparative study with conventional cholecystectomy and sympathetic blockade treatment. Surg Endosc 7: 416–419
Griffith J, Everitt N, Curley P, McMahon M (1993) Laparoscopic versus “open” cholecystectomy—reduced influence upon immune function and the acute phase response. Surg Endosc 7: 123
Harmon G, Senagore A, Kilbride M, Luchtefeld M, MacKeigan J, Warzynski M (1993) Cortisol and IL-6 response attenuated following laparoscopic colectomy. Surg Endosc 7: 121
Hill ADK, Banwell PE, Querechi A, Menzies-Gow N, Darzi A, Monson JRT, Guillou PJ (1993) IL-6 response in laparoscopic versus open herniorrhaphy. Surg Endosc 7: 232
Houdart R, Lavergne A, Galian A, Hautefeuille P (1983) [Anatomo-pathological evolution of single-layer end-to-end digestive anastomoses. A study of 210 colonic anastomoses in rats from the 2nd to the 180th day]. Gastroenterol Clin Biol 7: 465–473
Khansari DN, Murgo AJ, Faith RE (1990) Effects of stress on the immune system. Immunol Today 11: 170–175
Koruda MJ, Rolandelli RH (1990) Experimental studies on the healing of colonic anastomoses. J Surg Res 48: 504–515
Munday C, McGinn FP (1976) A comparison of polyglycolic acid and catgut sutures in rat colonic anastomoses. Br J Surg 63: 870–872
Murphy AA, Nager CW, Wujek JJ, Kettel LM, Torp VA, Chin HG (1992) Operative laparoscopy versus laparotomy for the management of ectopic pregnancy: a prospective trial. Fertil Steril 57: 1180–1185
NIH (1993) National Institutes of Health consensus development conference on gallstones and laparoscopic cholecystectomy. Am J Surg 165: 390–398
Papalois B, Antoniou P, Athanasiades C, Pappas D, Papdopoulou M, Vlachos J, Alexiou G (1993) Comparison of surgical stress in laparoscopic and “open” cholecystectomy. Surg Endosc 7: 214
Peters JH, Ellison EC, Innes JT, Liss JL, Nichols KE, Lomano JM, Roby SR, Front ME, Carey LC (1991) Safety and efficacy of laparoscopic cholecystectomy: a prospective analysis of 100 initial patients. Ann Surg 213: 3–12
Rademaker BM, Ringers J, Odoom JA, de Wit LT, Kalkman CJ, Ossting J (1992) Pulmonary function and stress response after laparoscopic cholecystectomy: comparison with subcostal incision and influence of thoracic epidural analgesia. Anesth Analg 75: 381–385
Trueblood HW, Nelsen TS, Kohatsy S, Oberhelman HA (1969) Wound healing in the colon: comparison of inverted and everted closures. Surgery 65: 919–930
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Berguer, R., Gutt, C.N. Laparoscopic colon surgery in a rat model. Surg Endosc 8, 1195–1197 (1994). https://doi.org/10.1007/BF00591049
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00591049