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Fate of3H- and14C-labelled A-4166 in pancreatic islets

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Abstract

The fate of3H- and14C-labelled A-4166 was examined in rat pancreatic islets. The net uptake of the meglitinide analogue by islets incubated for 60 min in the presence of 0.1 mM A-4166 and then submitted to repeated washes was close to 0.1 pmol/islet. It was significantly increased when the concentration ofd-glucose in the incubation medium was raised from 2.8 to 16.7 mM. No sizeable internalization of tritiated A-4166 into insulin-producing cells could be detected by autoradiography. These findings suggest that the interaction of A-4166 with the beta-cell may be restricted to its insertion on the plasma membrane and binding to sulphonylurea receptors.

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Authors and Affiliations

  1. Laboratory of Experimental Medicine, Brussels Free University, Erasmus Medical School, 808 Route de Lennik, B-1070, Brussels, Belgium

    F. Malaisse-Lagae & W. J. Malaisse

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  1. F. Malaisse-Lagae
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  2. W. J. Malaisse
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Malaisse-Lagae, F., Malaisse, W.J. Fate of3H- and14C-labelled A-4166 in pancreatic islets. Acta Diabetol 33, 298–300 (1996). https://doi.org/10.1007/BF00571569

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  • DOI: https://doi.org/10.1007/BF00571569

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