Summary
Specific antisera against glycogen phosphorylase, phosphofructokinase, aldolase, glyceraldehyde-phosphate dehydrogenase, enolase, lactate dehydrogenase, cytosolic and mitochondrial malate dehydrogenase from rabbit muscle were obtained from sheep. The gammaglobulins were used for indirect immunofluorescent localization of the respective enzymes in rabbit skeletal muscle and heart. In stretched skeletal muscle a cross-striation like distribution was observed for all enzymes studied. In the case of mitochondrial malate dehydrogenase this pattern is due to the staining of I-band mitochondria. In cross-sections, an intense staining of the sarcolemma and of subsarcolemmal mitochondria was observed. Comparative analyses with polarized light revealed that the cytosolic enzymes under study are distributed in the relaxed muscle fibre predominantly within the isotropic zones. The same distribution holds also for heart. In contracting muscle a decrease in cross-striated fluorescence and a faint staining of the interfibrillar spaces suggests a location also within the interfibrillar space.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnold, H., Nolte, J., Pette, D.: Quantitative and histochemical studies on the desorption and readsorption of aldolase in cross-striated muscle. J. Histochem. Cytochem. 17, 314–320 (1969)
Brandau, H., Pette, D.: Topische Muster von Enzymen des energieliefernden Stoffwechsels im quergestreiften Muskel. Enzymol. Biol. Clin. 6, 123–156 (1966)
Brosemer, R. W.: Immunofluorescent localization of two dehydrogenases in honeybee flight muscle. J. Histochem. Cytochem. 20, 266–271 (1972)
Clarke, F.M., Masters, C.J.: Multi-enzyme aggregates: new evidence for an association of glycolytic components. Biochim. biophys. Acta (Amst.) 327, 223–226 (1973)
Clarke, F.M., Masters, C.J.: On the association of glycolytic components in skeletal muscle extracts. Biochim. biophys. Acta (Amst.) 358, 193–207 (1974)
Dölken, G., Brdiczka, D., Pette, D.: Studies on the biosynthesis of malate dehydrogenase isozymes in rabbit liver. FEBS Lett. 35, 247–249 (1973)
Dölken, G., Leisner, E., Pette, D.: Turnover of malate dehydrogenase isozymes in rabbit liver and heart. Europ. J. Biochem. 47, 333–342 (1974)
Dölken, G., Pette, D.: Turnover of several glycolytic enzymes in rabbit heart, soleus muscle and liver. Hoppe-Seylers Z. physiol. Chem. 355, 289–299 (1974)
Dvorak, H.F., Cohen, R.B.: Localization of skeletal muscle phosphorylase using a fluorescent antibody technique and its correlation with histochemical observations. J. Histochem. Cytochem. 13, 454–460 (1965)
Emmart, E.W., Spicer, S.S., Turner, W.A., Henson, J.G.: The localization of glyceraldehyde-3-phosphate dehydrogenase within the muscle of the roach, periplaneta americana, by means of fluorescent antibody. Exp. Cell Res. 26, 78–97 (1962)
Fahimi, D., Amarasingham, C.R.: Cytochemical localization of lactic dehydrogenase in white skeletal muscle. J. Cell Biol. 22, 29–48 (1964)
Fahimi, H.D., Karnovsky, M.J.: Cytochemical localization of two glycolytic dehydrogenases in white skeletal muscle. J. Cell Biol. 29, 113–128 (1966)
Goldman, M.: Fluorescent antibody methods. New York and London: Academic Press 1968
Hofer, H.W.: Influence of enzyme concentration on the kinetic behaviour of rabbit muscle phosphofructokinase. Hoppe-Seylers Z. physiol. Chem. 352, 997–1004 (1971)
Hofer, H.W.: Relations between structure and catalytic activity of rabbit muscle phosphofructokinase. In: Reaction Mechanisms and Control Properties of Phosphotransferases. E. Hofmann and H.J. Böhme, eds., p. 367–384. Berlin: Akademie-Verlag 1973
Kekwick, R.A.: The serum proteins in multiple myelomatosis. Biochem. J. 34, 1248–1257 (1940)
Mayersbach, H. v.: Immunhistologische Methoden in der Histochemie. In: Handbuch der Histochemie, hrsg. v. Graumann, K.H. Neumann, Bd. I, 1, S. 599–639. Stuttgart: G. Fischer 1958
Mayersbach, v.H.: Immunhistologische Methoden in der Histochemie. In: Handbuch der Histochemie, hrsg. v. M. Vialli, J. Kruszynski, H. v. Mayersbach, Bd. I 2 Allgemeine Methodik. Stuttgart: G. Fischer 1966
Nairn, R.C.: Fluorescent protein tracing. Edinburgh and London: E. & S. Livingstone Ltd. 1964
Pette, D.: Some aspects of supramolecular organization of glycogenolytic and glycolytic enzymes in muscle. Acta histochem. (Jena), in press (1973)
Pette, D., Brandau, H.: Intracellular localization of glycolytic enzymes in cross-striated muscles of locusta migratoria. Biochem. biophys. Res. Commun. 9, 367–370 (1962)
Riggs, J.L., Loh, P.C., Eveland, W.C.: A simple fractionation method for preparation of fluorescein-labelled gammaglobulin. Proc. Soc. exp. Biol. (N.Y.) 105, 655–658 (1960)
Schulze, W., Will, H., Krause, E.-G., Tribulova, N.: Zur histochemischen Darstellung der Phosphorylase-Isoenzyme in Skelett- und Herzmuskel. Acta biol. med. germ. 28, 1063–1068 (1972)
Sigel, P., Pette, D.: Intracellular localization of glycogenolytic and glycolytic enzymes in white and red rabbit skeletal muscle. A gel film method for coupled enzyme reactions in histochemistry. J. Histochem. Cytochem. 17, 225–237 (1969)
Takeuchi, T., Kuriaki, H.: Histochemical detection of phosphorylase in animal tissues. J. Histochem. Cytochem. 3, 153–160 (1954)
Turner, D.C., Wallimann, T., Eppenberger, H.M.: A protein that binds specifically to the M-line of skeletal muscle is identified as the muscle form of creatine kinase. Proc. nat. Acad. Sci. (Wash.) 70, 702–705 (1973)
Author information
Authors and Affiliations
Additional information
This study was supported by Deutsche Forschungsgemeinschaft, Schwerpunktprogramm “Enzymdiagnostik” and Sonderforschungsbereich 138 “Biologische Grenzflächen und Spezifität”.
Rights and permissions
About this article
Cite this article
Dölken, G., Leisner, E. & Pette, D. Immunofluorescent localization of glycogenolytic and glycolytic enzyme proteins and of malate dehydrogenase isozymes in cross-striated skeletal muscle and heart of the rabbit. Histochemistry 43, 113–121 (1975). https://doi.org/10.1007/BF00492440
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00492440