Summary
Fibroblasts were cultured from autologous dermal connective tissue of albino rabbits and implanted (1×106–3×106 cells) into the vitreous space of 18 rabbit eyes under sterile surgical conditions. In 13 eyes a distinct growth of the cells occurred, and vitreous strands were formed. In 9 eyes a retinal detachment followed, and in some it was associated with traction of optic nerve tissue into the vitreous.
The proliferating fibroblasts produced collagen fibrils showing a band period of 45–55 nm. Intravitreal collagen formation is considered to be a pathologic phenomenon capable of inducing the development of vitreous retraction and retinal detachment.
Zusammenfassung
Züchtung von Fibroblasten aus dem Bindegewebe der Kaninchenhaut in Zellkulturen. Autologe Fibroblasten (1×106–3×106 Zellen) wurden unter sterilen Bedingungen in den Glaskörperraum der 18 Kaninchenaugen eingespritzt. In 13 Augen konnte eine deutliche Proliferation der Fibroblasten beobachtet werden, nach deren Entwicklung sich feinfädige Glaskörperverdichtungen bildeten. Eine Netzhautablösung folgte in 9 Augen, bisweilen durch eine Traktion des Nervus opticus in den Glaskörperraum kompliziert.
Die proliferierenden Fibroblasten produzierten kollagene Fibrillen mit periodischen Querstreifen von 45–55 nm. Die Bildung von Kollagen im Glaskörper ist ein pathologisches Phänomen, das zur Entwicklung einer Glaskörperschrumpfung und Netzhautablösung führen kann.
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This work was supported by the Swedish Medical Research Councilproject No. B74-12X-4207-01.
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Algvere, P., Kock, E. Experimental fibroplasia in the rabbit vitreous. Albrecht von Graefes Arch. Klin. Ophthalmol. 199, 215–222 (1976). https://doi.org/10.1007/BF00417290
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DOI: https://doi.org/10.1007/BF00417290