Abstract
Lipid metabolism takes place in the Golgi apparatus, but at a higher rate in female than in male rats. I therefore examined the Golgi apparatus by morphometric means for differences between the sexes at the light-and electron-microscopic level. The Golgi apparatus was stained in situ by a zinc-iodide-osmium method. The counts of the Golgi apparatus in cross-sections in female hepatocytes by light microscopy were approximately twice that in male hepatocytes. Upon ovariectomy, these counts were greatly reduced but were reestablished after estrogen supplement. To clarify this phenomenon, three-dimensional reconstructions of the Golgi apparatus were produced from electron-microscopic images of serially cut 160-nm sections. The Golgi apparatus of both male and ovariectomized females had the shape of a small ring, whereas it took the form of a large elongated cylinder in normal females and in castrated males after treatment with estrogen. The numerical difference in Golgi apparatus counts by light microscopy of in males and females is, therefore, apparently attributable to the size and shape of the Golgi apparatus, and is controlled by the estrogen level.
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References
Bareither ML, Verhage HG (1980) Effect of estrogen and progesterone on secretory granule formation and release in the endometrium of the ovariectomized cat. Biol Reprod 22:635–643
Barrau MD, Abel JH Jr, Verhage HG, Tietz WJ Jr (1975) Development of the endometrium during the estrous cycle in the bitch. Am J Anat 142:47–66
Brenner RM, Masler IA (1988) The primate oviduct and endometrium. In: Knobil E, Neill J et al. (eds) The physiology of reproduction. Raven Press, New York, pp 303–329
Chan L, Jackson RL, O'Malley BW, Means AR (1976) Synthesis of very low density lipoproteins in the cockerel. Effects of estrogen. J Clin Invest 58:368–379
Clermont Y, Xia L, Rambourg A, Turner JD, Hermo L (1993) Structure of the Golgi apparatus in stimulated and nonstimulated acinar cells of mammary glands of the rat. Anat Rec 237:308–317
Luskey KL, Brown MS, Goldstein JL (1974) Stimulation of the synthesis of very low density lipoproteins in rooster liver by estradiol. J Biol Chem 249:5939–5947
Murray MK (1992) The effect of estrogen and progesterone on structural changes in the uterine glandular epithelium of the ovariectomized sheep. Biol Reprod 47:408–417
Noda T, Ogawa K (1984) Golgi apparatus is one continuous organelle in pancreatic exocrine cell of mouse. Acta Histochem Cytochem 17:435–451
Ockner RK, Burnett DA, Lysenko N, Manning JA (1979) Sex differences in long chain fatty acid utilization and fatty acid binding protein concentration in rat liver. J Clin Invest 64:172–181
Rambourg A, Clermont Y, Chretien M, Olivier L (1993) Modulation of the Golgi apparatus in stimulated and nonstimulated prolactin cells of female rats. Anat Rec 235:353–362
Soler-Argilaga C, Wilcox HG, Heimberg M (1976) The effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitro. J Lipid Res 17:139–145
Tarlow DM, Watkins PA, Reed RE, Miller RS, Zwergel EE, Lane MD (1977) Lipogenesis and the synthesis and secretion of very low density lipoprotein by avian liver cells in nonproliferating monolayer culture. Hormonal effects. J Cell Biol 73:332–353
Watkins ML, Fizette N, Heimberg M (1972) Sexual influences on hepatic secretion of triglyceride. Biochem Biophys Acta 280:82–85
Wilcox HG, Woodside WF, Breen KJ, Knapp HR Jr, Heimberg M (1974) The effect of sex on certain properties of the very low density lipoprotein secreted by the liver. Biochem Biophys Res Commun 58:919–926
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Yamamoto, K. Sexual differences in the Golgi apparatus of rat hepatocytes: three-dimensional analysis. Cell Tissue Res 279, 459–463 (1995). https://doi.org/10.1007/BF00318158
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DOI: https://doi.org/10.1007/BF00318158