Summary
Autoradiographic studies revealed concentration of 3H 1,25(OH)2 vitamin D3 in nuclei of certain neurons in the spinal cord of adult and neonatal mice, fed a normal or a vitamin D deficient diet. Nuclear uptake and retention was strongest in motor neurons in lamina IX. Nuclear concentration also existed in neurons of lamina II, lamina VIII, lamina X and intermediate nucleus of the lateral column. The results indicate that these neurons are target neurons which contain nuclear receptors for 1,25(OH)2 vitamin D3. this suggests that 1,25(OH)2 vitamin D3 has direct genomic actions on the innervation of skeletal muscle by exerting related trophic, secretory, and electrophysiological effects. In addition, these data point to direct genomic actions of 1,25(OH)2 vitamin D3 on spinal sensory perception, and on certain autonomic functions. Nuclear binding in certain neurons in the peripheral ganglion of the trigeminal nerve further suggests that sensory perception is influenced by 1,25(OH)2 vitamin D3 not only at the level of the substantia gelatinosa, but also at the level of spinal ganglia.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boland R, Norman A, Ritz Z, Hasselbach W (1985) Presence of 1,25 dihydroxyvitamin D3 receptor in chick skeletal muscle myoblasts. Biochem Biophys Res Commun 128:305–311
Clark SA, Stumpf WE, Sar M, DeLuca HF, Tanaka Y (1980) Target cells for 1,25-dihydroxyvitamin D3 in the pancreas. Cell Tissue Res 209:515–520
Clark SA, Dame M, Kim YS, Stumpf WE, DeLuca HF (1985) 1,25-dihydroxyvitamin D3 in teeth of rats and humans: receptors and nuclear localization. Anat Rec 212:301–306
Clark SA, Stumpf WE, Bishop CW, DeLuca HF, Park DH, Joh TH (1986) The adrenal: a new target organ of the calciotropic hormone 1,25-dihydroxyvitamin D3. Cell Tissue Res 243:299–302
Clark SA, Stumpf WE, Sar M, DeLuca HF (1987) 1,25-dihydroxyvitamin D3 target cells in immature panereatic islets. Am J Physiol 253:E99-E105
Duncan GE, Stumpf WE (1984) Target neurons for 3H-corticosterone in the rat spinal cord. Brain Res 307:321–326
Garcia-Segura LM, Baetens D, Roth J, Norman AW, Orci L (1984) Immunohistochemical mapping of calcium-binding protein immunoreactivity in the rat central nervous system. Brain Res 296:75–86
Heyburn PJ, Peacock M, Casson IF, Crilly RG, Taylor GA (1983) Vitamin D metabolism in post-menopausal women and their relationship to the myopathic electromyogram. Eur J Clin Invest 13:41–44
Kim YS, Clark SA, Stumpf WE, DeLuca HF (1985) Nuclear uptake of 1,25-dihydroxyvitamin D3 in developing rodent teeth: an autoradiographic study. Anat Rec 212:3301–3306
Mallette LE, Patten BM, Engel WK (1975) Neuromuscular disease in secondary hyperparathyroidism. Ann Int Med 82:474–483
Peacock M (1977) The treatment of proximal myopathy with 1,25-dihydroxyvitamin D3 or its synthetic analogue 1alpha hydroxyvitamin D in various endocrinological diseases. Q J Med 56:556
Sar M, Stumpf WE (1977) Androgen concentration in motor neurons of cranial nerves and spinal cord. Science 197:77–79
Sar M, Stumpf WE (1980) Simultaneous localization of 3H-estradiol and neurophysin I or arginine vasopressin in hypothalamic neurons demonstrated by a combined technique of dry mount autoradiography and immunohistochemistry. Neurosci Lett 17:179–184
Sar M, Stumpf WE, DeLuca HF (1980) Thyrotropes in the pituitary are target cells for 1,25 (OH)2 vitamin D3. Cell Tissue Res 209:161–166
Sar M, Stumpf WE (1981) Combined autoradiography and immunohistochemistry for simultaneous localization of radioactively labeled steroid hormones and antibodies in the brain. J Histochem Cytochem 29(1A):161–166
Schott GD, Wills MR (1976) Muscle weakness in osteomalacia. Lancet 1:626–629
Simpson RU, Thomas GA, Arnold AJ (1985) Identification of 1,25-dihydroxyvitamin D3 receptors and activities in muscle. J Biol Chem 260:8882–8891
Stumpf WE (1976) Techniques for the autoradiography of diffusible compounds. In: Prescott DM (ed) Methods in Cell Biology, vol XIII. Academic Press, New York, pp 171–193
Stumpf WE, Downs TW (1987) Nuclear receptors for 1,25 (OH)2 vitamin D3 in thymus reticular cells studied by autoradiography. Histochemistry 87:367–369
Stumpf WE, O'Brien LP (1987a) Autoradiographic studies with 1,25-dihydroxyvitamin D3 in thyroid and associated tissues of the neck region. Histochemistry 87:53–58
Stumpf WE, O]Brien LP (1987b) 1,25 (OH)2 vitamin D3 sites of action in the brain: an autoradiographic study. Histochemistry 87:393–406
Stumpf WE, Sar M (1979) Glucocorticosteroid and mineralcorticosteroid hormone target sites in the brain: Autoradiographic studies with corticosterone, aldosterone and dexamethasone. In: Jones MT, Dallman MF, Gillham B, Chattopadhyay S (eds) Interactions within the brain-pituitary adrenocortical system. Academic Press, New York, pp 137–147
Stumpf WE, Sar M, Reid FA, Tanaka Y, DeLuca HF (1979) Target cells for 1,25-dihydroxyvitamin D3 in intestinal tract, stomach, kidney, skin, pituitary, and parathyroid. Science 206:1188–1190
Stumpf WE, Sar M, Narbaitz R, Reid FA, DeLuca HF, Tanaka Y (1980a) Cellular and subcellular localization of 1,25 (OH)2 vitamin D3 in rat kidney: comparison with that of parathyroid hormone and estradio. Proc Natl Acad Sci USA 77:1149–1153
Stumpf WE, Sar M, Clark SA, Lieth E, DeLuca HF (1980b) Target neurons for 1,25 (OH)2 vitamin D3 in brain and spinal cord. Neuroendocrin Lett 2:297–301
Stumpf WE, Sar M, DeLuca HF (1981) Sites of action of 1,25 (OH)2 vitamin D3 identified by thaw-mount autoradiography. In: Hormonal control of calcium metabolism. Cohn DV, Talmage RV, Matthews Jr JL, (eds) Excerpta Medica, Amsterdam, pp 222–229
Stumpf WE, Sar M, Clark SA, DeLuca HF (1982) Brain target sites for 1,25-dihydroxyvitamin D3. Science 215:1403–1405
Stumpf WE, Clark SA, Sar M, DeLuca HF (1984) Topographical and developmental studies on 1,25 (OH)2 vitamin D3 target sites in skin. Cell Tissue Res 238:489–496
Stumpf WE, Sar M, Chen K, Morin J, DeLuca HF (1987a) Sertoli cells in the testis and epithelium of the ductuli efferentes are targets for 3H 1,25 (OH)2 vitamin D3: an autoradiographic study. Cell Tissue Res 247:453–455
Stumpf WE, Sar M, O'Brien LP, Morin JK (1987b) Pyloric gastrin-producing cells and pyloric sphincter muscle cells are nuclear targets for 3H 1,25(OH)2 vitamin D3: studied by autoradiography and immunohistochemistry. Histochemistry, submitted
Wassner SJ, Li JB, Sperduto A, Norman ME (1983) Vitamin D deficiency, hypocalcemia, and increased skeletal muscle degradation in rats. J Clin Invest 72:102–112
Author information
Authors and Affiliations
Additional information
Supported in part by USPHS grants NS 09914 and HD 03110
Rights and permissions
About this article
Cite this article
Stumpf, W.E., Clark, S.A., O'Brien, L.P. et al. 1,25(OH)2 vitamin D3 sites of action in spinal cord and sensory ganglion. Anat Embryol 177, 307–310 (1988). https://doi.org/10.1007/BF00315837
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00315837