Abstract
Sequencing of the VP1 of a large number of subtypes of foot-and-mouth disease virus (FMDV) has revealed the presence of a conserved arginine-glycine-aspartic acid (RGD) sequence located in a highly exposed region. This sequence has been shown to be essential for the interaction of certain extracellular matrix and adhesion proteins with a superfamily of cell-surface receptors called integrins. We have examined the effects of synthetic peptides containing the RGD sequence on the binding of eight different subtypes of FMDV to tissue culture cells. The results showed that such peptides inhibited viral adsorption by 50–80%. The inhibition was dose dependent but not as great as that achieved by using a saturating amount of virus as an inhibitor. Substitution of other amino acids for any of the three main residues lowered the inhibitory properties of the peptides. These results suggest that the RGD sequence in FMDV VP1 appears to be important for the interaction of virus with cellular receptor sites.
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Pierschbacher, M.D. and Ruoslahti, E., Nature 309, 30–33, 1984.
Pierschbacher, M.D. Hayman, E.G., and Ruoslahti, E., J. Cell Biochem. 28, 115–126, 1985.
Ruoslahti, E. and Pierschbacher, M.D., Cell 44, 517–518, 1986.
Hynes, R.O., Cell 48, 549–554, 1987.
Ruoslahti, E. and Pierschbacher, M.D., Science 238, 491–497, 1987.
Pytela, R., Pierschbacher, M.D., Ginsberg, M.H., Plow, E.F., and Ruoslahti, E., Science 231, 1559–1562, 1986.
Pytela, R., Pierschbacher, M.D., and Ruoslahti, E., Proc. Natl. Acad. Sci. USA 82, 5766–5770, 1985.
Rice, C.M. and Strauss, J.H., Proc. Natl. Acad. Sci. USA 78, 2062–2066, 1981.
Baxt, B. and Bachrach, H.L., Virology 104, 42–55, 1980.
Baxt, B. and Bachrach, H.L., Virology 116, 391–405, 1982.
Sekiguchi, K., Franke, A.J., and Baxt, B., Arch. Virol. 74, 53–64, 1982.
Baxt, B., Morgan, D.O., Robertson, B.H., and Timpone, C.A., J. Virol. 51, 298–305, 1984.
Baxt, B., Virus Res. 7, 257–271, 1987.
Acharya, R., Fry, E., Stuart, D., Fox, G., Rowlands, D., and Brown, F., Nature 337, 709–716, 1989.
Hogle, J.M., Chow, M., and Filman, D.J., Science 229, 1358–1365, 1985.
Rossmann, M.G., Arnold, E., Erickson, J.W., Frankenberger, E.A., Griffith, J.P., Hecht, H.J., Johnson, J.E., Kamer, G., Luo, M., Mosser, A.G., Rueckert, R.R., Sherry, B., and Vriend, G., Nature 317, 145–153, 1985.
Luo, M., Vriend, G., Kamer, G., Minor, I., Arnold, E., Rossmann, M.G., Boege, U., Scraba, D.G., Duke, G.M., and Palmenberg, A.C., Science 235, 182–191, 1987.
Colonno, R.J., Condra, J.H., Mizutani, S., Callahan, P.L., Davies, M.E., and Murcko, M.A., Proc. Natl. Acad. Sci. USA 85, 5449–5453, 1988.
Luo, M., Rossmann, M.G., and Palmenberg, A.C., Virology 116, 503–514, 1988.
Weddell, G.N., Yansura, D.G., Dowbenko, D., Hoatlin, M.E., Grubman, M.J., Moore, D.M., and Kleid, D.G., Proc. Natl. Acad. Sci. USA 82, 2618–2622, 1985.
Fox, G., Parry, N.R., Barnett, P.V., McGinn, B., Rowlands, D.J., and Brown, F., J. Gen. Virol. 70, 625–637, 1989.
Surovoi, A. Yu., Ivanov, V.T., Chepurkin, A.V., Ivanyuschenkov, V.N., and Dryagalin, N.N. Translated from Bioorganicheskaya Khimiya 14, 965–968, 1988.
Swaney, L.M., Vet. Microb. 18, 1–14, 1988.
Strohmaier, K., Franze, R., and Adman, K.H., J. Gen. Virol. 59, 295–306, 1982.
Baxt, B., Vakharia, V., Moore, D.M., Franke, A.J., and Morgan, D.O., J. Virol. 63, 2143–2151, 1989.
Robertson, B.H., Grubman, M.J., Weddell, G.N., Moore, D.M., Welsh, J.D., Fischer, T., Dowbenko, D.J., Yansura, D.G., Small, B., and Kleid, D., J. Virol. 54, 651–660, 1985.
Argraves, W.S., Suzuki, S., Arai, H., Thompson, K., Pierschbacher, M.D., and Ruoslahti, E., J. Cell. Biol. 105, 1183–1190, 1987.
Thomas, A., Woortmrijer, R.J., Puijk, W., and Barteling, S.J., J. Virol. 62, 2782–2789, 1988.
Akiyama, S.K. and Yamada, K.M., J. Biol. Chem. 260, 4492–4500, 1985.
Akiyama, S.K. and Yamada, K.M., J. Biol. Chem. 260, 10402–10405, 1985.
Yamada, K.M. and Kennedy, D.W., J. Cell. Physiol. 130, 21–28, 1987.
Hautanen, A., Gailit, J., Mann, D.M., and Ruoslahti, E., J. Biol. Chem. 264, 1437–1442, 1989.
Ruoslahti, E., Ann. Rev. Biochem 57, 375–413, 1988.
D'Souza, S.E., Ginsberg, M.H., Burke, T.A., Lam, S.C.T., and Plow, E.F., Science 242, 91–93, 1988.
Robertson, B.H., Moore, D.M., Grubman, M.J., and Kleid, D.G., J. Virol. 46, 311–316, 1983.
Greve, J.M., Davis, G., Meyer, A.M., Forte, C.P., Yost, S.C., Marlor, C.W., Karnarck, M.E., and McClelland, A., Cell 58, 839–847, 1989.
Staunton, D.E., Merluzzi, V.J., Rothlein, R., Barton, R., Marlin, S.D., and Springer, T.A., Cell 58, 849–853, 1989.
Mendelsohn, C.L., Wimmer, E., and Racaniello, V.R., Cell 58, 855–865, 1989.
Tomassini, J.E., Graham, D., DeWitt, C.M., Lineberger, D.W., Rodkey, J.A., and Colonno, R.J., Proc. Natl. Acad. Sci. USA 86, 4907–4911, 1989.
Marlin, S.D. and Springer, T.A., Cell 51, 813–819, 1987.
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Baxt, B., Becker, Y. The effect of peptides containing the arginine-glycine-aspartic acid sequence on the adsorption of foot-and-mouth disease virus to tissue culture cells. Virus Genes 4, 73–83 (1990). https://doi.org/10.1007/BF00308567
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DOI: https://doi.org/10.1007/BF00308567