Summary
Antibodies to human type II collagen were examined in the sera of 105 patients with rheumatoid arthritis (RA), 44 patients with systemic lupus erythematosus (SLE) and 11 patients who fulfilled the criteria of both diseases (RA-SLE overlap), using a solid-phase radioimmunoassay (RIA). The frequencies of antibodies to native and denatured human type II collagen were 20% and 27% in RA, 14% and 16% in SLE, and 45% and 36% in RA-SLE overlap. The specificity of the antibodies was further examined by inhibition with native and denatured type II collagen, by immunoblotting on native and denatured type II collagen, and by immunoblotting on cyanogen-bromide derived polypeptides of type II collagen. We could not identify any disease-specific patterns of reactivity. Thus, in the three disease groups the antibody response was polyclonal; there were antibody populations that reacted with native and/or denatured collagen, and epitopes could be assigned to at least three CB peptides, CB10.5, CB11 and CB8.
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References
Steffen C (1970) Considerations of the pathogenesis of rheumatoid arthritis as collagen autoimmunity. Z Immunitätsforsch 139:219–227
Clague RB, Shaw MJ, Holt PJL (1980) Incidence of serum antibodies to native type I and type II collagens in patients with inflammatory arthritis. Ann Rheum Dis 39:201–206
Trentham DE, Kammer GM, McCune WJ, David JR (1981) Autoimmunity to collagen: a shared feature of psoriatic and rheumatoid arthritis. Arthritis Rheum 24:1363–1369
Stuart JM, Huffstutter EH, Townes AS, Kang AH (1983) Incidence and specificity of antibodies of types I, II, III, IV, and V collagen in rheumatoid arthritis and other rheumatic diseases as measured by 125I-radioimmunoassay. Arthritis Rheum 26:832–840
Rowley M, Tait B, Mackay IR, Cunningham T, Phillips B (1986) Collagen antibodies in rheumatoid arthritis. Significance of antibodies to denatured collagen and their association with HLA-DR4. Arthritis Rheum 29:174–184
Choi EKK, Getenby PA, McGill NW, Bateman JF, Cole WG, York JR (1988) Autoantibodies to type II collagen: occurrence in rheumatoid arthritis, other arthritides, autoimmune connective tissue diseases, and chronic inflammatory syndromes. Ann Rheum Dis 47:313–322
Trentham DE, Kammer GM, McCune WJ, David JR (1981) Autoimmunity to collagen. A shared feature of psoriatic and rheumatoid arthritis. Arthritis Rheum 24:1363–1369
Trentham DE, Dynesius RA, Rocklin RE, David JR (1978) Cellular sensitivity to collagen in rheumatoid arthritis. N Engl J Med 299:327–332
Stuart JM, Postlethwaite AE, Townes AS, Kang AH (1980) Cell-mediated immunity to collagen and collagen α chains in rheumatoid arthritis and other rheumatic diseases. Am J Med 69:13–18
Golds EE, Stephen IBM, Esdaile JM, Strawczynski H, Poole AR (1983) Lymphocyte transformation to connective tissue antigens in adult and juvenile rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, systemic lupus erythematosus, and a nonarthritic control population. Cell Immunol 82:196–209
Charriere G, Hartmann DJ, Vignon E, Vignon E, Ronziere M-C, Herbage D, Ville G (1988) Antibodies to types I, II, IX, and XI collagen in the serum of patients with rehumatic diseases. Arthritis Rheum 31:325–332
Rosenberg AM, Hunt DWC, Petty RE (1984) Antibodies to native and denatured type II collagen in children with rheumatic diseases. J Rheumatol 11:425–431
Rowley MJ, Gershwin ME, Mackay IR (1988) Collagen antibodies in juvenile arthritis and adult rheumatoid arthritis: differences in levels and type-specificity. J Rheumatol 15:289–293
Trentham DE, Townes AS, Kang AH (1977) Autoimmunity to type II collagen: an experimental model of arthritis. J Exp Med 146:857–868
Courtenay JS, Dalloman MJ, Dayan AD Martin A, Mosedale B (1980) Immunization against heterologous type II collagen induces arthritis in mice. Nature 283:666–667
Yoo TJ, Kim S-Y, Stuart JM, Floyd RA, Olsen GA, Cremer MA, Kang AH (1988) Induction of arthritis in monkeys by immunization with type II collagen. J Exp Med 168:777–782
Holmdahl R, Jansson J, Larsson E, Rubin K, Klareskog L (1986) Homologous type II collagen induces chronic and progressive arthritis in mice. Arthritis Rheum 29:106–113
Terato K, Hasty KA, Cremer MA, Cremer MA, Stuart JM, Townes AS, Kang AH (1985) Collagen-induced arthritis in mice: localization of an arthritogenic determinant to a fragment of the type II collagen molecule. J Exp Med 162:637–646
Englert ME, Ferguson KM, Suarez CR, Sapp TM, Oronsky AL, Kerwar SS (1987) Type II collagen arthritis: identification of arthritogenic epitopes using fractionated anticollagen IgG. Cell Immunol 105:447–453
Terato K, Arai H, Shimozuru Y, Fukada T, Tanaka H, Watanabe H, Nagai Y, Fijimoto K, Okubo F, Cho F, Honjo S, Cremer MA (1989) Sex-linked differences in susceptibility of cynomolgus monkeys to type II collagen-induced arthritis. Arthritis Rheum 32:748–758
Buckee C, Morgan K, Ayad S, Collins I, Clague RB, Holt PJL (1990) Diversity of antibodies to type II collagen in patients with rheumatoid arthritis: detection by binding to α-chains and to cyanogen bromide peptides. Br J Rheumatol 29:254–258
Terato K, Shimozuru Y, Katayama K, Takemitsu Y, Yamashita I, Miyatsu M, Fujii K, Sagara M, Kobayashi S, Goto M, Nishioka K, Miyasaka N, Nagai Y (1990) Specificity of antibodies to type II collagen in rheumatoid arthritis. Arthritis Rheum 33:1493–1499
Choi EKK, Gatenby PA, Bateman JF, Cole GW (1990) Antibodies to type II collagen in SLE: a role in the pathogenesis of deforming arthritis? Immunol Cell Biol 68:27–31
Brand CA, Rowley MJ, Tait BD, Muirden KD, Whittingham SF (1992) Coexistent rheumatoid arthritis and systemic lupus erythematosus: clinical, serological and phenotypic features. Ann Rheum Dis 51:173–176
Scott PG, Veis A (1976) The cyanogen bromide peptides of bovine soluble and insoluble collagens. Connect Tissue Res 4:107–116
Ramshaw JAM, Werkmeister JA (1988) Electrophoresis and electroblotting of native collagens. Anal Biochem 168:82–87
Bornstein P, Sage H (1980) Structurally distinct collagen types. Annu Rev Biochem 49:957–1003
Miller EJ, Rhodes RK (1982) Preparation and characterization of the different types of collagen. Methods Enzymol 82:33–64
Terato K, Cremer MA, Hasty KA, Kang AH, Hasty DL, Townes AS (1985) Physicochemical and immunological studies of the renatured α1 (II) chains and isolated cyanogen bromide peptides of type II collagen. Collagen Res Rel 5:469–480
Benjamini E, Michaeli D, Leung CY, Wong K, Scheuenstuhl H (1973) Immunochemical studies with synthetic peptides related to collagen. Immunochemistry 10:629–638
Watson WC, Townes AS (1985) Genetic susceptibility to murine collagen II autoimmune arthritis: proposed relationship to the IgG2 autoantibody subclass response, complement C5, major histocompatibility complex (MHC), and non-MHC loci. J Exp Med 162:1878–1891
Watson WC, Brown PS, Pitcock JA, Townes AS (1987) Passive transfer studies with type II collagen antibody in B10.D2/old and new line and C57B1/6 normal and beige (Chediak-Higashi) strains: evidence of important roles for C5 and multiple inflammatory cell types in the development of erosive arthritis. Arthritis Rheum 30:460–465
Ludgate M, Vaasart G (1990) Molecular genetics of three thyroid autoantigens: thyroglobulin, thyroid peroxidase and the thyrotropin receptor. Autoimmunity 7:301–211
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Rowley, M.J., Mackay, I.R., Brand, C.A. et al. Epitope specificity of antibodies to type II collagen in rheumatoid arthritis and systemic lupus erythematosus. Rheumatol Int 12, 65–69 (1992). https://doi.org/10.1007/BF00300979
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DOI: https://doi.org/10.1007/BF00300979