Summary
In a series of four studies, adult female Swiss-Webster mice were used to measure the effects of salmon calcitonin on two biochemical indices of local and systematic bone formation: (1) skeletal alkaline phosphatase activity—in serum and in extracts of calvaria and tibiae, and (2) calvarial collagenase-digestible protein synthesis—measured, acutely, in vitro. Subcutaneous calcitonin doses ranged from 50 to 400 mU/mouse/day (0.95–18.1 U/kg/day), and treatment schedules were continuous (daily) for 2–14 days, acute, or intermittent (2 days/week for 6 weeks). The effects of calcitonin on these bone formation indices (skeletal alkaline phosphatase and collagenase-digestible protein synthesis) were biphasic with respect to dose and treatment time, being increased in response to short-term, low-dose treatment, but not long-term, continuous treatment. The effects of long-term intermittent calcitonin treatment were dose-dependent increases in skeletal alkaline phosphatase in calvaria and serum (r=0.948, P< 0.02, and r=0.960, P< 0.01, respectively).
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Farley, J.R., Hall, S.L., Herring, S. et al. Two biochemical indices of mouse bone formation are increased, in vivo, in response to calcitonin. Calcif Tissue Int 50, 67–73 (1992). https://doi.org/10.1007/BF00297300
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DOI: https://doi.org/10.1007/BF00297300