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In vitro differentiation of diakinesis figures in human testis

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Summary

Fragments of tissue from human testicular biopsies were incubated at 31°C in medium supplemented with 10% w/v foetal calf serum and enriched with 4 mM L-glutamine. The tissue was supported during growth at the gas/medium interface. Autoradiography was used to follow the progress of differentiating spermatogonia. Activity of the testicular epithelium was observed throughout 21 days of culture. Many diakinesis figures were observed between 10–14 days growth in culture.

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Authors and Affiliations

  1. Sub-Department of Medical Genetics, University of Sheffield, 117, Manchester Road, S10, 5DN, Sheffield, England

    Diana Curtis

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  1. Diana Curtis
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Curtis, D. In vitro differentiation of diakinesis figures in human testis. Hum Genet 59, 406–411 (1981). https://doi.org/10.1007/BF00295480

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  • DOI: https://doi.org/10.1007/BF00295480

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