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Differential sensitivity of Fanconi anaemia lymphocytes to the clastogenic action of cis-diamminedichloroplatinum (II) and trans-diamminedichloroplatinum (II)

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Summary

Fanconi anaemia (FA) lymphocytes were tested for their susceptibility to chromosomal breakage by cis-diamminedichloroplatinum (II) [cis-Pt(II)] and its stereoisomer trans-diamminedichloroplatinum (II) [trans-Pt(II)]. Unlike trans-Pt(II), which is a rather inefficient clastogen, cis-Pt(II) is very efficient in inducing chromosomal breakage in FA cells at concentrations that hardly affect control cells. As both cis-Pt(II) and trans-Pt(II) are capable of inducing DNA interstrand crosslinks but only cis-Pt(II) can induce DNA intra-strand crosslinks, this result suggests that FA cells may be specifically sensitive to the intrastrand type of DNA crosslink.

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Authors and Affiliations

  1. Institute of Human Genetics, Faculty of Medicine, Free University, NL-1007 MC, Amsterdam, The Netherlands

    E. H. A. Poll, F. Arwert, H. Joenje & A. H. Wanamarta

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  1. E. H. A. Poll
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  2. F. Arwert
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  3. H. Joenje
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  4. A. H. Wanamarta
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Poll, E.H.A., Arwert, F., Joenje, H. et al. Differential sensitivity of Fanconi anaemia lymphocytes to the clastogenic action of cis-diamminedichloroplatinum (II) and trans-diamminedichloroplatinum (II). Hum Genet 71, 206–210 (1985). https://doi.org/10.1007/BF00284574

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  • DOI: https://doi.org/10.1007/BF00284574

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