Skip to main content
SpringerLink
Log in

Human uromucoid

I. Quantitative immunoassay

  • Published:
Urological Research Aims and scope Submit manuscript

Summary

Uromucoid, the urinary glycoprotein, consists of an immunoelectrophoretically distinct low molecular weight protein and a macromolecular component that can be precipitated with sodium chloride. Uromucoid purified by salt precipitation had chemical and physico-chemical properties similar to those reported previously. The high molecular weight fragment gives a precipitin line in a ring test using an anti-uromucoid serum, but there is a visible reaction by the Ouchterlony technique only after it has been cleaved with sodium dodecyl sulfate. The molecules obtained by the latter method are related immunologically to native uromucoid and are completely identical to the urinary low molecular weight protein. A method is described in detail for the quantitative determination of uromucoid in human urine by single radial immunodiffusion and electroimmunoassay.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zühlke, V., Herman, G.: Immunologische Untersuchungen zur Proteinurie gesunder Personen mit Nachweis von Nierenproteinen. Z. ges. exp. Med. 149, 333 (1969).

    Google Scholar 

  2. Tamm, I., Horsfall, F. L. Jr.: Characterization and separation of an inhibitor of viral hemagglutination present in urine. Proc. Soc. exp. Biol. 74, 108 (1950).

    Google Scholar 

  3. Fletcher, A. P., Neuberger, A., Ratcliffe, W. A.. Tamm-Horsfall urinary glycoprotein Biochem. J. 120, 417 (1970).

    Google Scholar 

  4. Friedmann, T., Johnson, P.: Further characterization of the subunit of Tamm-Horsfall mucoprotein. Biochim. biophys. Acta 130, 355 (1966).

    Google Scholar 

  5. Stevenson, F. K.: The viscosity of Tamm-Horsfall mucoprotein and its relation to cystic fibrosis. Clin. chim. Acta 23, 441 (1969).

    Google Scholar 

  6. Maxfield, M.: Urinary glycoprotein (T and H); in: Gottschalk, A.: Glycoproteins, Chapt. 11, 446. Amsterdam: Elsevier Publishing Co. 1966.

    Google Scholar 

  7. Boyce, W. H., King, J. S., Jr., Fielden, M. L.: Total nondialyzable solids (TNDS) in human urine. IX. Immunochemical studies of the R-1 “uromucoid” fraction. J. clin. Invest. 40, 1453 (1961).

    Google Scholar 

  8. Keutel, H. J., Heremans, J., Vaerman, J. P.: Möglichkeiten zur Fällung und Differenzierung der Harnkolloide, in: H. Peeters (ed.): Protides of the biological fluids, p. 326 Proceeding of the 8th Colloquium, Bruges. Amsterdam: Elsevier 1960.

    Google Scholar 

  9. Heremans, M. Th., Vaerman, J. P., Heremans, J. F.: Studies on normal urinary colloids; in: Peeters, H., (ed.): Protides of the biological fluids, p. 396. Proceedings of the 7th Colloquium, Bruges. Amsterdam: Elsevier 1959.

    Google Scholar 

  10. Keutel, H. J., King, J. S. Jr., Boyce, W. H.: Further studies of uromucoid in normal and stone urine. Urol. int. 17, 324 (1964).

    Google Scholar 

  11. Ferrante, N. D., Rich, C.: The mucopolysaccharide of normal human urine. Clin. chim. Acta 1, 519 (1956).

    Google Scholar 

  12. Anderson, A. J., Maclagan, N. F.: The isolation and estimation of urinary mucoproteins. Biochem. J. 59, 638 (1955).

    Google Scholar 

  13. Boyce, W. H., King, J. S., Little, J. M., Jr., Artom, C.: Total nondialyzable solids (TNDS) in human urine. II. A method for reproducible fractionation. J. clin. Invest. 37, 1658 (1958).

    Google Scholar 

  14. Porter, K. R., Tamm, I.: Direct visualization of a mucoprotein component of urine. J. biol. Chem. 212, 135 (1955).

    Google Scholar 

  15. Odin, L.: Carbohydrate residue of a urine mucoprotein inhibiting influenza virus haemagglutination. Nature (Lond.) 170, 663 (1952).

    Google Scholar 

  16. Boyce, W. H., Swanson, M.: Biocolloids of urine in health and in calculous disease. II. Electrophoretic and biochemical studies of a mucoprotein insoluble in molar sodium chloride. J. clin. Invest. 34, 1581 (1955).

    Google Scholar 

  17. Fletcher, A. P., Mc Laughlin, J. E, Ratcliffe, W. A., Woods, D. A.: The chemical composition and electron microcopic appearance of a protein derived from urinary casts. Biochim. biophys. Acta 214, 299 (1970).

    Google Scholar 

  18. Tamm, I., Horsfall, F. L., Jr.: A mucoprotein derived from human urine which reacts with influenza, mumps, and Newcastle disease viruses. J. exp. Med. 95, 71 (1952).

    Google Scholar 

  19. Friedmann, T., Johnson, P.: The disaggregation of Tamm-Horsfall mucoprotein by acetic acid. Biochim. biophys. Acta 121, 292 (1966).

    Google Scholar 

  20. Stevenson, F. K., Kent, P. W.: Subunits of Tamm-Horsfall glycoprotein. Biochem. J. 116, 791 (1970).

    Google Scholar 

  21. Fletcher, A. P., Neuberger, A., Ratcliffe, W. A.: Tamm-Horsfall urinary glycoprotein. The subunit structure. Biochem. J. 120, 425 (1970).

    Google Scholar 

  22. Boyce, W. H., Garvey, F. K.: The amount and nature of the organic matrix in urinary calculi. A review. J. Urol. 76, 213 (1956).

    Google Scholar 

  23. Südhof, H., Krieger, D, Schultz, E.: Über das Harnmucoprotein. Dtsch. Arch. klin. Med. 205, 285 (1958).

    Google Scholar 

  24. Mc Kenzie, J. K., Patel, R., Mc Queen, E. G.: The excretion rate of Tamm-Horsfall urinary mucoprotein in normals and in patients with renal disease. Austr. Ann. Med. 13, 32 (1964).

    Google Scholar 

  25. Bichler, K.-H.: Uromukoidausscheidung. Harnsteinsymposium, Bonn 1972.

  26. Bichler, K.-H., Hirschhäuser, C., Porzsolt, F., Naber, K.: Methoden zur Untersuchung der Proteinfraktionen im Urin. Klin. Wschr. 50, 1056 (1972).

    Google Scholar 

  27. Zwisler, O., Biel, H.: Elektrophorese in horizontalem Polyacrylamidgel. Z. klin. Chem. 4, 58 (1966).

    Google Scholar 

  28. Schultze, H. E., Schmidtberger, R., Haupt, H.: Untersuchungen über die gebundenen Kohlenhydrate in isolierten Plasmaproteiden. Biochem. Z. 329, 490 (1958).

    Google Scholar 

  29. Scheidegger, J. J.: Une micro-méthode de l'immunoélectrophoresis. Int. Arch. Allergy 7, 103 (1955).

    Google Scholar 

  30. Ouchterlony, Ö.: Diffusion-in-gel methods for immunological analysis. Prog. Allergy 6, 30 (1962).

    Google Scholar 

  31. Mancini, G., Carbonara, A., Heremans, J. F.: Immunochemical quantitation of antigens by single radial immundiffusion. Immunochemistry 2, 235 (1965)

    Google Scholar 

  32. Becker, W.: Determination of antisera titres using the single radial immundiffusion method. Immunochemistry 6, 539 (1969).

    Google Scholar 

  33. Laurell, C.-B.: Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analyt. Biochem. 15, 45 (1966).

    Google Scholar 

  34. Tamm, I., Burgher, J. C., Horsfall, F. L. Jr.: Ultracentrifugation studies of a urinary mucoprotein which reacts with various viruses. J. biol. Chem. 212, 125 (1955).

    Google Scholar 

  35. Maxfield, M.: Physicochemical study in water of a mucoprotein with virus-inhibiting activity. Science 123, 1087 (1958).

    Google Scholar 

  36. McQueen, E. G.: The nature of urinary casts. J. clin. Pathol. 15, 367 (1962).

    Google Scholar 

  37. Marr, A. M. S., Neuberger, A., Ratcliffe, W. A.: Rabbit Tamm-Horsfall urinary glycoprotein. Chemical composition and subunit structure. Biochem. J. 122, 623 (1971).

    Google Scholar 

  38. Grant, G. H.: The proteins of normal urine. J. clin. Pathol. 10, 360 (1957).

    Google Scholar 

  39. Vaerman, J. P., Heremans, J. F.: Etude immuno-électrophorétique de l'uromucoide. Experientia 15, 226 (1959).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Urologische Universitätsklinik and Behringwerke AG, Marburg/Lahn, Germany (FRG)

    K. H. Bichler, H. Haupt, G. Uhlemann & H. G. Schwick

Authors
  1. K. H. Bichler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Haupt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Uhlemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. G. Schwick
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bichler, K.H., Haupt, H., Uhlemann, G. et al. Human uromucoid. Urol. Res. 1, 50–59 (1973). https://doi.org/10.1007/BF00256119

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00256119

Key words

Search

Navigation