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Multiple steroid receptors in human breast cancer

II. Estrogen and progestin receptors in 672 primary tumors

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  • Steroid Receptors in Breast Cancer
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Summary

Estrogen (E) and progestin (P) receptors (R) were investigated in 672 human primary breast tumors (664 from women and 8 from men). Premenopausal patients have both a lower frequency of ER positive tumors (64%) and a lower ER content (80±63 fmol/mg protein) than postmenopausal women (74% and 249±351 fmol/mg protein). All eight cases of male breast cancer were ER-positive. PR was found in 45% of the tumors. In contrast to ER, the presence of PR and the absolute PR level are not related to patient age. Except for the month of March, the mean monthly ER values do not fluctuate in postmenopausal patients: A peak is observed in the ER content of breast cancer from patients who underwent surgery during the month of March. Absolute ER content appears to be statistically unrelated to the tumorous cellular density (TCD); expression of the results as femtomoles per milligram of protein gives an underestimate of the actual receptor concentration. TCD must thus be considered in interpretation of the ER content. The results are in agreement with the idea that the steroid receptors are a characteristic of a population of cancer cells within a breast tumor and that ovarian factors are implicated in the steroid receptor content.

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References

  1. Braunsberg, H.: Factors influencing the estimation of estrogen receptors in human malignant breast tumors. Eur. J. Cancer 11, 499–506 (1975)

    Google Scholar 

  2. Braunsberg, H., James, V. H. T., Jamieson, C. W., Desai, S., Carter, A. E., Hulbert, M.: Effect of age and menopausal status on estimates of estrogen binding by human malignant breast tumors. Br. Med. J. 1974 N, 745–747

  3. Burton, R.: A study of the conditions and mechanism of diphenylamine reaction for the colorimetric estimation of DNA. Biochem. J. 62, 315–323 (1956)

    Google Scholar 

  4. Cikes, M.: Expression of hormone receptors in cancer cells: A hypothesis. Eur. J. Cancer 14, 211–215 (1978)

    Google Scholar 

  5. Cole, P., Cramer, D., Yen, S., Paffenbarger, R., McMahon, B., Brown, J.: Estrogen profiles of premenopausal women with breast cancer. Cancer Res. 38, 745–748 (1978)

    Google Scholar 

  6. Frantz, A. G.: Prolactin. N. Engl. J. Med. 298, 201–207 (1978)

    Google Scholar 

  7. Gerschenson, L. E., Conne, E., Murai, J. T.: Regulation of the cell cycle by diethylstilbestrol and progesterone in cultured endometrial cells. Endocrinology 100, 1468–1471 (1977)

    Google Scholar 

  8. Hahnel, R., Twaddle, E.: Estradiol binding by human breast carcinoma cytosols: the influence of commonly used reference parameters on the results. Eur. J. Cancer 14, 125–131 (1978)

    Google Scholar 

  9. Hahnel, R., Vivian, A. B.: Biochemical and clinical experience with the estimation of estrogen receptors in human breast carcinomas. In: Estrogen receptors in human breast cancer. McGuire, W. L., Carbone, P. P., Vollmer, E. P. (eds.), pp. 205–235. New York: Raven Press 1975

    Google Scholar 

  10. Hahnel, R., Twaddle, R., Vivian, A. B.: Estrogen receptors in human breast cancer. II. In vitro binding of estradiol by benign and malignant tumors. Steroids 18, 681–687 (1971)

    Google Scholar 

  11. Heuson, J. C., Longeval, E., Mattheiem, W. H., Deboel, M. C., Sylvester, R. J., Leclercq, G.: Significance of quantitative assessment of estrogen receptors for endocrine therapy in advanced breast cancer. Cancer 39, 1971–1977 (1977)

    Google Scholar 

  12. Hilf, R., Wittliff, J. L., Rector, W. D., Savlov, E. D., Hall, T. C., Orlando, R. H.: Studies on certain cytoplasmic enzymes and specific estrogen receptors in human breast cancer and in non-malignant diseases of breast. Cancer Res. 33, 2054–2058 (1973)

    Google Scholar 

  13. Horwitz, K. B., McGuire, W. L.: Predicting response to endocrine therapy in human breast cancer: A hypothesis. Science 189, 726–727 (1975)

    Google Scholar 

  14. Horwitz, K. B., McGuire, W. L.: Estrogen and progesterone: their relationship in hormone-dependent breast cancer. In: Progesterone receptors in normal and neoplasic tissues. McGuire, W. L. et al. (eds.), pp. 103–124. New York: Raven Press 1977

    Google Scholar 

  15. Hsueh, A. J. W., Peck, E. J., Clark, J. H.: Progesterone antagonism of estrogen receptor and estradiol-induced uterine growth. Nature 254, 337–338 (1975)

    Google Scholar 

  16. Hughes, A., Jacobson, H. I., Wagner, R. K., Jungblut, P. W.: Ovarian-independent fluctuations of estradiol receptor levels in mammalian tissues. Mol. Cell. Endocrinol. 5, 379–388 (1976)

    Google Scholar 

  17. Jensen, E. V., Suzuki, T., Kawashima, T., Stumpf, W. E., Jungblut, P. W., DeSombre, E. R.: A two-step mechanism for the interaction of estradiol with rat uterus. Proc. Natl. Acad. Sci. USA 59, 632–638 (1968)

    Google Scholar 

  18. Jensen, E. V., Smith, S., DeSombre, E. R.: Hormone dependency in breast cancer. J. Steroid Biochem. 7, 911–917 (1976)

    Google Scholar 

  19. Kiang, D. T., Kennedy, B. J.: Factors affecting estrogen receptors in breast cancer. Cancer 40, 1571–1576 (1977)

    Google Scholar 

  20. Kim, U., Depowski, M. J.: Progression from hormone dependance to autonomy in mammary tumors as an in vivo manifestation of sequential clonal selection. Cancer Res. 35, 2068–2077 (1975)

    Google Scholar 

  21. Kimura, J.: Effect of progesterone on cell division in chemically induced endometrial hyperplasia and adenocarcinoma in mice. Cancer Res. 38, 78–82 (1978)

    Google Scholar 

  22. Korenman, S. G.: Estrogen receptor assay in human breast cancer. J. Natl. Cancer Inst. 55, 543–545 (1975)

    Google Scholar 

  23. Lowry, O. H., Rosebrough N. J., Farr, A. L., Randall, R. J.: Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 265–270 (1951)

    CAS  PubMed  Google Scholar 

  24. Martin, P. M., Rolland, P. H., Jacqumier, J., Rolland, A. M., Toga, M.: Estradiol receptors (ER) in human breast cancer. Neccessity of correlations with anatomopathologic examinations. Rev. Endocr. Rel. Cancer Suppl. April 1978 51–56 (1978)

    Google Scholar 

  25. Martin, P. M., Rolland, P. H., Jacquemier, J., Rolland, A. M., Toga, M.: Multiple steroid receptors in human breast cancer. I. Technological features. Biomedecine 28, 278–287 (1978)

    Google Scholar 

  26. Martin, P. M., Horwitz, K. B., Rayan, D. S., McGuire, W. L.: Phytoestrogen interaction with estrogen receptors in human breast cancer cells. Endocrinology 103, 1860–1867 (1978)

    Google Scholar 

  27. McGuire, W. L., Chamness, G. C.: Studies on the estrogen receptor in breast cancer. In: Receptors for reproductive hormones. O'Malley, B. W., Means, A. R. (eds.), pp. 113–136. Plenum Press 1973

  28. McGuire, W. L., Chamness, G. C., Costlow, M. E., Sheperd, R. E.: Hormone dependance in breast cancer. Metabolism 23, 75–100 (1974)

    Google Scholar 

  29. McGuire, W. L., Carbone, P. P., Sears, M. E., Escher, G. C.: Estrogen receptors in human breast cancer: An overiew. In: Estrogen receptors in human breast cancer. McGuire, W. L. et al. (eds.), pp. 1–7. New York: Raven Press 1975

    Google Scholar 

  30. McGuire, W. L., Carbone, P. P., Vollmer, E. P. (eds.): Estrogen receptors in human breast cancer. New York: Raven Press 1975

    Google Scholar 

  31. McGuire, W. L., Horwitz, K. B., Chamness, G. C., Zava, D. T.: A physiological role for estrogen and progesterone in breast cancer. J. Steroid Biochem. 7, 875–882 (1976)

    Google Scholar 

  32. McGuire, W. L., Horwitz, K. B., Pearson, O. H., Segaloff, A.: Current status of estrogen and progesterone receptors in breast cancer. Cancer 39, 2934–2947 (1977)

    Google Scholar 

  33. McGuire, W. L., Raynaud, J. P., Baulieu, E. E. (eds.): Progesterone receptors in normal and neoplastic tissues. (Progress in cancer research and therapy, Vol. 4). New York: Raven Press 1977

    Google Scholar 

  34. MacMahon, B., Cole, P., Brown, J.: Etiology of human breast cancer: A review. J. Natl. Cancer Inst. 50, 21–42 (1973)

    Article  Google Scholar 

  35. Rosen, R. P., Mendez-Botet, C. J., Nisselbaum, J. S., Schwartz, H. K., Urban, J. A.: Estrogen receptor protein in lesions of the male breast. Cancer 37, 1866–1868 (1976)

    Google Scholar 

  36. Saez, S., Mayer, M.: Facteurs hormonaux de prévision d'hormonal-dependance du cancer du sein. Bull. Cancer (Paris) 64, 557–566 (1977)

    Google Scholar 

  37. Saez, S., Chouvet, C., Sakai, F., Mayer, M., Colon, J., Pommateau, E., Cheiz, F., Clavel, M.: Les récepteurs d'oestradiol dans le cancer mammaire chez la femme. In: Hormones and breast cancer, Vol. 55, pp. 45–56. Paris: Inserm 1975

    Google Scholar 

  38. Sakai, F., Saez, S.: Existence of receptors bound to endogenous estradion in breast cancers of premenopausal and postmenopausal women. Steroids 27, 99–110 (1976)

    Google Scholar 

  39. Scarff, R. W., Torloni, H.: An histological typing of breast tumors. Coll. International Histological Classification of tumors, no. 2, pp. 204–408. Geneva: WHO 1968

    Google Scholar 

  40. Sherman, B. M., Korenman, S. G.: Inadequate corpus luteum function. A pathophysiological interpretation of human breast cancer epidemiology. Cancer 33, 1306–1312 (1974)

    Google Scholar 

  41. Sherman, B. M., West, J. H., Korenman, S. G.: The menopausal transition: Analyses of LH, FSH, estradiol and progesterone concentrations during menstrual cycles of older women. J. Clin. Endocrinol. Metab. 42, 629–636 (1976)

    Google Scholar 

  42. Spaeren, U., Olnes, S., Brennhoud, I., Efskind, J., Pihl, A.: Content of estrogen receptors in human cancers. Eur. J. Cancer 9, 353–357 (1973)

    Google Scholar 

  43. Terenius, L.: Estrogen and progesterone binders in human and rat mammary carcinoma. Eur. J. Cancer 9, 291–305 (1973)

    Google Scholar 

  44. Terenius, L., Johansson, H, Rimsten, A., Thoren, L.: Malignant and benign human mammary disease. Estragen binding in relation to clinical data. Cancer 33, 1364–1368 (1974)

    Google Scholar 

  45. Vignon, F., Rochefort, H.: Regulation of estrogen receptors in ovarian-dependent rat mammary tumors. I. Effects of castration and prolactin. Endocrinology 98, 722–729 (1976)

    Google Scholar 

  46. Wittliff, J. L., Hilf, R., Brooks, W. F., Savlov, E. D., Hall, T. C., Orlando, R. A.: Specific estrogen-binding capacity of the cytoplasmic receptor in normal and neoplasic breast tissues of human. Cancer Res. 32, 1983–1988 (1971)

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire des Récepteurs et des Stéroides Hormonaux, Institut Paoli-Calmettes, 232 Bd. Sainte-Marguerite, F-13273, Marseille, Cedex 2, France

    P. M. Martin, P. H. Rolland, J. Jacquemier, A. M. Rolland & M. Toga

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  1. P. M. Martin
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  2. P. H. Rolland
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  3. J. Jacquemier
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  4. A. M. Rolland
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  5. M. Toga
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Martin, P.M., Rolland, P.H., Jacquemier, J. et al. Multiple steroid receptors in human breast cancer. Cancer Chemother. Pharmacol. 2, 107–113 (1979). https://doi.org/10.1007/BF00254082

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