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Blood-aqueous barrier in pseudoexfoliation syndrome: evaluation by immunohistochemical staining of endogenous albumin

  • Clinical Investigations
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Abstract

• Background: Alterations of the integrity of the blood-aqueous barrier (BAB) are frequent findings in eyes with pseudoexfoliation syndrome (PSX). • Methods: Immunohistochemical staining for the demonstration of albumin was used to analyze the BAB in 10 eyes with PSX without previous intraocular surgery and in 10 age-matched normal control eyes. • Results: In eyes with PSX, small amounts of albumin were detected along the anterior surface of the iris in 7, in the anterior chamber in 1, along the ciliary epithelium in 4, and in the trabecular meshwork in 9 of 10 eyes. PSX material was also immunoreactive. In the 10 normal control eyes, albumin was detected anterior to the iris stroma in 1 eye, in the anterior chamber in 2 eyes, in the trabecular meshwork in 1 eye, but not internal to the ciliary epithelium. • Conclusions: Our findings indicate that impairment of the BAB in PSX can be localized at the level of the iris and, less frequently or to a lesser extent, at the level of the ciliary body.

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References

  1. Baltatzis S, Georgopoulos G, Theodossiadis P (1993) Fibrin reaction after extracapsular cataract extraction: a statistical evaluation. Eur J Ophthalmol 3: 95–97

    Google Scholar 

  2. Brooks AMV, Gillies WE (1983) Fluorescein angiography and fluorophotometry of the iris in pseudoexfoliation of the lens capsule. Br J Ophthalmol 67: 249–254

    Google Scholar 

  3. Brooks AMV, Gillies WE (1987) The development of microneovascular changes in the iris in pseudoexfoliation of the lens capsule. Ophthalmology 94: 1090–1097

    Google Scholar 

  4. Drolsum L, Davanger M, Haaskjold E (1994) Risk factors for an inflammatory response after extracapsular cataract extraction and posterior chamber IOL. Acta Ophthalmol (Copenh) 72: 21–26

    Google Scholar 

  5. Ghosh M, Speakman JS (1974) The iris in senile exfoliation of the lens. Can J Ophthalmol 9: 289–297

    Google Scholar 

  6. Harnish J-P (1977) Exfoliation material in different sections of the eye. Graefe's Arch Clin Exp Ophthalmol 203: 181–190

    Google Scholar 

  7. Helbig H, Schlötzer-Schrehardt U, Noske W, Kellner U, Foerster MH, Naumann GOH (1994) Anterior-chamber hypoxia and iris vasculopathy in pseudoexfoliation syndrome. Germ J Ophthalmol 3: 148–153

    Google Scholar 

  8. Johnson DH, Brubaker RF (1982) Dynamics of aqueous humor in the syndrome of exfoliation with glaucoma. Am J Ophthalmol 93: 629–634

    Google Scholar 

  9. Johnson M, Gong H, Freddo TF, Ritter N, Kamm R (1993) Serum proteins and aqueous outflow resistance in bovine eyes. Invest Ophthalmol Vis Sci 34: 3549–3557

    Google Scholar 

  10. Konstas AGP, Marshall GE, Lee WE (1991) Iris vasculopathy in exfoliation syndrome. An immunocytochemical study. Acta Ophthalmol 69: 472–483

    Google Scholar 

  11. Küchle M, Schlötzer-Schrehardt U, Naumann GOH (1991) Occurrence of pseudoexfoliative material in parabulbar structures in pseudoexfoliation syndrome. Acta Ophthalmol 69: 124–130

    Google Scholar 

  12. Küchle M, Nguyen NX, Horn F, Naumann GOH (1992) Quantitative assessment of aqueous flare and aqueous “cells” in pseudoexfoliation syndrom. Acta Ophthalmol 70: 201–208

    Google Scholar 

  13. Küchle M, Ho TS, Nguyen NX, Hannappel E, Naumann GOH (1994) Protein quantification and SDS-PAGE electrophoresis in aqueous humor of pseudoexfoliation eyes. Invest Ophthalmol Vis Sci 35: 748–752

    Google Scholar 

  14. Küchle M, Vinores JA, Henderer SD, Green WR (1995) Immunohistochemical evaluation of the integrity of the blood-aqueous barrier in normal and rubcotic human eyes. Graefe's Arch Clin Exp Ophthalmol (in press)

  15. Menchini U, Ferrari E, Lanzetta P (1994) Fluoroangiographic abnormalities of the iris related to glaucoma in pseudoexfoliation syndrome. Invest Ophthalmol Vis Sci Suppl 35: 1853

    Google Scholar 

  16. Morrison JC, Green WR (1988) Light microscopy of the exfoliation syndrome. Acta Ophthalmol 66 [Suppl 184]: 5–27

    Google Scholar 

  17. Naumann GOH, Erlanger Augenblätter Group (1988) Exfolition syndrome as a risk factor for vitreous loss in extracapsular cataract surgery. Acta Ophthalmol 55 [Suppl 184]: 129

    Google Scholar 

  18. Naumann GOH, Küchle M, Schönherr U, Erlanger Augenblätter-Gruppe (1989) Pseudoexfoliationssyndrom als Risikofaktor für Glaskörperverlust bei der extrakapsulären Kataraktextraktion. Fortschr Ophthalmol 86: 543–545

    Google Scholar 

  19. Pasquale LR, Green WR (1993) Exfoliation syndrome: Slit lamp biomicroscopy and pthological findings of the anterior segment. New Trends Ophthalmol 8: 117–129

    Google Scholar 

  20. Pouliquen P, Robinet A, Colin J, Cochener B, Charpentier D (1992) Syndrome exfoliatif et chirurgie de la cataracte. J Fr Ophthalmol 15: 171–176

    Google Scholar 

  21. Quigley HA, Green WR (1979) The histology of human glaucoma cupping and optic nerve damage: clinicopathologic correlation in 21 eyes. Ophthalmology 86: 1803–1827

    Google Scholar 

  22. Ringvold A (1969) Electron microscopy of the wall of iris vessels in eyes with and without exfoliation syndrome (pseudo exfoliation of the lens capsule). Virchows Arch Abt A Pathol Anat 348: 328–341

    Google Scholar 

  23. Ringvold A, Davanger M (1981) Iris neovascularisation in eyes with pseudoexfoliation syndrome. Br J Ophthalmol 65: 138–141

    Google Scholar 

  24. Sampaolesi R (1956) Note sur la diffusion de la fluoréscene dans le corps vitré. Ophthalmologica 131: 359–364

    Google Scholar 

  25. Sampaolesi R (1960) Neue Untersuchungen über das PseudoKapselhäutchen-Glaukom (Glaucoma capsulare). Ber Dtsch Ophthalmol Ges 62: 177–183

    Google Scholar 

  26. Schlötzer-Schrehardt U, Küchle M, Naumann GOH (1991) Electron microscopic identification of pseudoexfoliation material in extrabulbar tissue. Arch Ophthalmol 109: 565–570

    Google Scholar 

  27. Schötzer-Schrehardt U, Dörfler S, Naumann GOH (1992) Immunohistochemical localization of basement membrane components in pseudoexfoliation material of the lens capsule. Curr Eye Res 11: 343–355

    Google Scholar 

  28. Schlötzer-Schrehardt U, Dörfler S, Naumann GOH (1992) Immunohistochemical localization of blood-aqueous barrier dysfunction in eyes with pseudoexfoliation syndrome. Invest Ophthalmol Vis Sci [Suppl] 33: 731

    Google Scholar 

  29. Schlötzer-Schrehardt UM, Koca MR, Naumann GOH, Volkholz H (1992) Pseudoexfolition syndrome — ocular manifestation of a systemic disease? Arch Ophthalmol 110: 1752–1765

    Google Scholar 

  30. Schlötzer-Schrehardt U, Küchle M, Dörfler S, Naumann GOH (1993) Pseudoexfoliative material in the eyelid skin of pseudoexfoliation-suspect patients: a clinico-histopathological correlation. Germ J Ophthalmol 2: 51–60

    Google Scholar 

  31. Schönherr U, Küchle U, Händel A, Lang GK, Naumann GOH (1990) Pseudoexfoliationssyndrom mit und ohne Glaukom als ernstzunehmender Risikofaktor bei der extrakapsulären Kataraktextraktion. Eine prospektive klinische Studie. Fortschr Ophthalmol 87: 588–590

    Google Scholar 

  32. Shakib M, Ashton N, Blach R (1965) Electron microscopic study of pseudoexfoliation of the lens capsule. II. Iris and ciliary body. Invest Ophthalmol Vis Sci 4: 154–161

    Google Scholar 

  33. Shimizu T (1985) Changes of iris vessels in capsular glaucoma: three-dimensional and electron microscopic studies. Jap J Ophthalmol 29: 434–452

    Google Scholar 

  34. Spinelli D, de Felice GP, Vigasio F, Coggi G (1985) The iris vessels in the exfoliation syndrome: ultrastructural changes. Exp Eye Res 41: 449–455

    Google Scholar 

  35. Streeten BW, Gibson SA, Li ZY (1986) Lectin binding to pseudoexfoliative material and the ocular zonules. Invest Ophthalmol Vis Sci 27: 1516–1521

    Google Scholar 

  36. Streeten BW, Gibson SA, Dark AJ, Wallace RN, Li Z-Y, Hoepner JA (1990) Pseudoexfoliative fibrillopathy in the skin of patients with ocular pseudoexfoliation. Am J Ophthalmol 110: 490–499

    Google Scholar 

  37. Streeten BW, Bookman L, Ritch R, Prince AM, Dark AJ (1992) Pseudoexfoliation fibrillopathy in visceral organs of a patient with pseudoexfoliation syndrome. Arch Ophthalmol 110: 1752–1762

    Google Scholar 

  38. Streeten BW, Qi Y, Wallace RN (1994) Pseudoexfoliation (PSX) syndrome: further evidence of a systemic fibrillopathy with a major component of fibrillin 15. Invest Ophthalmol Vis Sci Suppl 35: 1785

    Google Scholar 

  39. Tada H, Takahashi N, Kimura Y, Numaga T, Yoneya S (1988) Anterior segment fluorophotometry in pseudoexfoliation syndrome. Jpn J Clin Ophthalmol 42: 698–699

    Google Scholar 

  40. Tarkkanen AHA (1986) Exfoliation syndrome. Trans Ophthalmol Soc UK 105: 233–236

    Google Scholar 

  41. Uusitalo M, Kivelä T, Tarkkanen A (1993) Immunoreactivity of exfoliation material for the cell adhesion-related HNK-1 carbohydrate epitope. Arch Ophthalmol 111: 1419–1423

    Google Scholar 

  42. Uusitalo M, Kivelä T, Tarkkanen A (1994) The HNK-1 epitope in the inner connective tissue layer of the human ciliary body in exfoliation syndrome and various types of glaucoma. Graefe's Arch Clin Exp Ophthalmol 232: 8–15

    Google Scholar 

  43. Vannas A (1969) Fluorescein angiography of the vessels of the iris in pseudoexfoliation of the lens capsule, capsular glaucoma and some other forms of glaucoma. Acta Ophthalmol [Suppl] 105: 9–75

    Google Scholar 

  44. Vannas A (1972) Vascular changes in pseudoexfoliation of the lens capsule and capsular glaucoma. A fluorescein angiographic and electron microscopic study. Graefe's Arch Clin Exp Ophthalmol 184: 248–253

    Google Scholar 

  45. Vinores SA, Gadegbeku C, Campochiaro PA, Green WR (1989) Immunohistochemical localization of blood-retinal breakdown in human diabetics. Am J Pathol 134: 231–235

    Google Scholar 

  46. Vinores SA, Campochiaro PA, Lee A, McGehee R, Gadegbeku C, Green WR (1990) Localization of blood-retinal barrier breakdown in human pathologic specimens by immunohistochemical staining for albumin. Lab Invest 62: 742–750

    Google Scholar 

  47. Wälinder PEK, Olivius EOP, Nordell SI, Thorburn WE (1989) Fibrinoid reaction after extracapsular cataract extraction and relationship to exfoliation syndrome. J Cataract Refract Surg 15: 526–530

    Google Scholar 

  48. Zetterström C, Olivestedt G, Lundvall A (1992) Exfoliation syndrome and extracapsular cataract extraction with implantation of posterior chamber lens. Acta Ophthalmol 70: 85–90

    Google Scholar 

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Authors and Affiliations

  1. Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Michael Küchle, Stanley A. Vinores, Jeremy Mahlow & W. Richard Green

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  1. Michael Küchle
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  2. Stanley A. Vinores
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  3. Jeremy Mahlow
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  4. W. Richard Green
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Küchle, M., Vinores, S.A., Mahlow, J. et al. Blood-aqueous barrier in pseudoexfoliation syndrome: evaluation by immunohistochemical staining of endogenous albumin. Graefe's Arch Clin Exp Ophthalmol 234, 12–18 (1996). https://doi.org/10.1007/BF00186513

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  • DOI: https://doi.org/10.1007/BF00186513

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