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Pax-1 in the development of the cervico-occipital transitional zone

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Abstract

The Pax-1 gene has been found to play an important role in the development of the vertebral column. The cervico-occipital transitional zone is a specialized region of the vertebral column, and malformations of this region have frequently been described in humans. The exact embryonic border between head and trunk is a matter of controversy. In order to determine a possible role of Pax-1 in the development of the cervico-occipital transitional zone we studied the expression of this gene in a series of quail embryos and murine fetuses with in situ hybridization and immunohistochemistry. Pax-1 is expressed in all somites of the embryo, including the first five occipital ones. During embryonic days 3–5 the gene is down-regulated in the caudal direction within the first five somites, whereas more caudally Pax-1 is strongly expressed in the cells of the perinotochordal tube. In 5-day-old quail embryos, the cartilaginous anlage of the basioccipital bone has developed and there is no more expression of Pax-1 in this region. The fusion of the dens axis with the body of the axis also coincides with switching off of the Pax-1 gene. More caudally, the gene is continuously expressed in the intervertebral discs of murine embryos and therefore seems to be important for the process of resegmentation. Quail embryos do not possess permanent intervertebral discs. “Hyper-” or “hyposegmentation” defects may be explained by an over- or under-expression of Pax-1 during development. We also reinvestigated the border between the head and trunk in chick embryos by performing homotopical grafting experiments of the 5th somite between chick and quail embryos. Grafted quail cells formed mainly the caudal end of the basioccipital bone. They were also located in the cranial half of the ventral atlantic arch, and only a few cells were found in the tip of the dens axis.

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Authors and Affiliations

  1. Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Albertstrasse 17, D-79104, Freiburg, Germany

    Jörg Wilting, Cecilia Ebensperger, Thomas S. Müller & Bodo Christ

  2. Division of Molecular Immunology, Center of Biomedical Science, School of Medicine, Chiba University, 1-8-1 Inohana, Chuoku, 260, Chiba, Japan

    Haruhiko Koseki

  3. Astra Pain Control AB, Novum Unit, S-14157, Huddinge, Sweden

    Johan Wallin

Authors
  1. Jörg Wilting
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  2. Cecilia Ebensperger
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  3. Thomas S. Müller
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  4. Haruhiko Koseki
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  5. Johan Wallin
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  6. Bodo Christ
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Wilting, J., Ebensperger, C., Müller, T.S. et al. Pax-1 in the development of the cervico-occipital transitional zone. Anat Embryol 192, 221–227 (1995). https://doi.org/10.1007/BF00184746

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  • DOI: https://doi.org/10.1007/BF00184746

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