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Involvement of cell-cell adhesion molecules in liver colonization by metastatic murine lymphoma/lymphosarcoma variants

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Metastatic variant sublines of the murine RAW117 large cell lymphoma or lymphosarcoma have been established in vitro by sequential cycles of harvesting of liver tumor nodules after intravenous inoculation of tumor cell suspensions into syngeneic BALB/c mice. After five and tenin vivo selections for liver colonization, variant sublines RAW117-H5 and -H10, respectively, were established, and these formed significantly more surface liver tumors than the parental RAW117-P line. RAW117 sublines were tested for their abilities to adhere to embryonic mouse liver or brain cells in anin vitro cell-cell adhesion assay. Liver colonizing RAW117-H10 cells adhered with greater selectivity to liver cells than to brain cells. Parental RAW 117-P cells were more homotypically adhesive, but they were nonselective in their organ cell adhesion properties. We examined RAW117 cells for the presence of liver cross-reactive antigens using polyclonal xenoantibody preparations directed against embryonic murine liver cells. These antibody preparations block organ-specific homotypic adhesion of embryonic murine liver cellsin vitro. The amount of fetal liver antigen(s) expressed on RAW117 sublines correlated with liver colonization potentials (H10 > H5 > P) in quantitative absorption assays. Treatment of the highly metastatic RAW117-H10 subline with polyclonal anti-embryonic murine liver F(ab′)2 or Fab′ antibody fragments had no effect on RAW117-H10 cell viability or growthin vitro orin vivo, but inhibited liver colonization (median liver tumor colonies reduced from > 200 to 0) and prolonged life expectancy. In contrast, pretreatment of RAW 117-H 10 cells with polyclonal anti-H-2 did not modify thein vivo biologic properties of these metastatic cells.

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References

  1. Brackenbury, R., Thiery, J.-P., Rutishauser, U., and Edelman, G. M., 1977, Adhesion among neural cells of the chick embryo. I. An immunological assay for molecules involved in cell-cell binding.Journal of Biological Chemistry,252, 6835–6840.

    Google Scholar 

  2. Brunson, K. W., and Nicolson, G. L., 1978, Selection and biologic properties of malignant variants of murine lymphosarcoma. Journal of the National Cancer Institute, 61, 1499–1503.

    Google Scholar 

  3. Chen, T. R., 1977,In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.Experimental Cell Research,104, 255–262.

    Google Scholar 

  4. Fidler, I. J., and Nicolson, G. L., 1981, Immunobiology of experimental metastatic melanoma.Cancer Biology Review,2, 171–234.

    Google Scholar 

  5. Galfre, G., Howe, S. C., Milstein, C., Butcher, G. W., and Howard, J. C., 1977, Antibodies to major histocompatibility antigens produced by hybrid cell lines.Nature, London,266, 550–552.

    Google Scholar 

  6. Grady, S. R., and McGuire, E. J., 1976, Intercellular adhesive selectivity. III. Species selectivity of embryonic liver intercellular adhesion.Journal of Cell Biology,71, 96–106.

    Google Scholar 

  7. Grady, S. R., Nielsen, L. D., and McGuire, E. J., 1982, Organ and class specificity of cell adhesion blocking antisera.Experimental Cell Research,142, 169–180.

    Google Scholar 

  8. Hermann, S. H., and Mescher, M. F., 1979, Purification of the H-2K k molecule of the murine major histocompatibility complex.Journal of Biological Chemistry,254, 8713–8716.

    Google Scholar 

  9. Kieran, M. W., and Longenecker, B. M., 1983, Organ specific metastasis with special reference to avian systems.Cancer Metastasis Reviews,2, 165–182.

    Google Scholar 

  10. Littlefield, J. W., 1964, Selection of hybrids from matings of fibroblastsin vitro and their presumed recombinants.Science,145, 709–710.

    Google Scholar 

  11. Miner K. M., and Nicolson, G. L., 1983, Differences in the sensitivities of murine metastatic lymphoma/lymphosarcoma cells to macrophage-mediated cytolysis and/or cytostasis.Cancer Research,43, 2063–2071.

    Google Scholar 

  12. Miner, K. M., Reading, C. L., and Nicolson, G. L., 1981,In vivo andin vitro production and detection of monoclonal antibodies to surface components on metastatic variants of murine tumor cells.Invasion Metastasis,1, 158–174.

    Google Scholar 

  13. Miner, K. M., Walter, H., and Nicolson, G. L., 1981, Subfractionation of malignant variants of metastatic murine lymphosarcoma cells by counter-current distribution in two-polymer aqueous phases.Biochemistry,20, 6244–6249.

    Google Scholar 

  14. Nicolson, G. L., 1982, Cancer metastasis: Organ colonization and the cell surface properties of malignant cells.Biochimica et Biophysica Acta,695, 113–176.

    Google Scholar 

  15. Nicolson, G. L., Mascali, J. J., and McGuire, E. J., 1982, Metastatic RAWI17 lymphosarcoma as a model for malignant-normal cell interactions. Possible roles for cell surface antigens in determining the quantity and location of secondary tumors.Oncodevelopmental Biology and Medicine,4, 149–159.

    Google Scholar 

  16. Nicolson, G. L., and Posts, G., 1983, Tumor implantation and invasion at metastatic sites.International Review of Experimental Pathology,25, 77–181.

    Google Scholar 

  17. Nicolson, G. L., Reading, C. L., and Brunson, K. W., 1980, Blood-borne tumor metastasis: Some properties of selected tumor cell variants of differing malignancies. InTumor progression, edited by R. G. Crispen (Amsterdam: Elsevier North-Holland), pp. 31–48.

    Google Scholar 

  18. Nisonoff, A., Markus, G., and Wissler, F. C., 1961, Separation of univalent fragments of rabbit antibody by reduction of a single, labile disulphide bond.Nature, London,189, 293–295.

    Google Scholar 

  19. Nisonoff, A., Wissler, F. C., Lipman, L. N., and Woernley, B. L., 1960, Properties of the major component of a peptic digest of rabbit antibody.Science,132, 1770–1771.

    Google Scholar 

  20. Obrink, B., Kuhlenschmidt, M. S., and Roseman, S., 1977, Adhesive specificity of juvenile rat and chicken liver cells and membranes.Proceedings of the National Academy of Science, U.S.A.,74, 1077–1081.

    Google Scholar 

  21. Ossowski, L., and Reich, E., 1983, Antibodies to plasminogen activator inhibit human tumor metastasis.Cell,35, 611–619.

    Google Scholar 

  22. Raschke, W. C., Ralph, P., WATSON, J., Sklar, M., and Coon, H., 1975, Oncogenic transformation of murine lymphoid cells byin vitro infection with Abelson leukemia virus.Journal of the National Cancer Institute,54, 1249–1253.

    Google Scholar 

  23. Reading, C. L., Belloni, P. N., and Nicolson, G. L., 1980, Selection and in vivo properties of lectin-attachment variants of malignant murine lymphosarcoma cell lines.Journal of the National Cancer Institute,64, 1241–1249.

    Google Scholar 

  24. Reading, C. L., Brunson, K. W., Torriani, M., and Nicolson, G. L., 1980, Malignancies of metastatic murine lymphosarcoma cell lines and clones correlate with decreased cell surface display of RNA tumor virus envelope glycoprotein gp70.Proceedings of the National Academy of Sciences U.S.A.,77, 5943–5947.

    Google Scholar 

  25. Reading, C. L., Kraemer, P. M., Miner, K. M., and Nicolson, G. L., 1983,In vivo andin vitro properties of malignant variants of RAW117 metastatic murine lymphoma/lymphosarcoma.Clinical and Experimental Metastasis,1, 135–151.

    Google Scholar 

  26. Shearman, P. J., Gallatin, W. M., and Longenecker, B. M., 1980, Detection of a cell-surface antigen correlated with organ-specific metastasis.Nature, London,286, 267–269.

    Google Scholar 

  27. Shearman, P. J., and Longenecker, B. M., 1980, Selection of virulence and organ-specific metastasis of herpes virus-transformed lymphoma cells.International Journal of Cancer,25, 363–369.

    Google Scholar 

  28. Shearman, P. J., and Longenecker, B. M., 1981, Clonal variation and functional correlation of organ-specific metastasis and an organ-specific metastasis associated antigen.International Journal of Cancer,27, 387–395.

    Google Scholar 

  29. Sugarbaker, E. V., 1981, Patterns of metastasis in human malignancies.Cancer Biology Review,2, 235–278.

    Google Scholar 

  30. Schirrmacher, V., Cheingsong-Popov, R., and Arnheiter, H., 1980, Hepatocyte-tumor cell interactionin vitro. I. Conditions for rosette formation and inhibition by antiH-2 antibody.Journal of Experimental Medicine,151, 984–989.

    Google Scholar 

  31. Thiery, J. P., Brackenbury, R., Rutishauser, U., and Edelman, G., 1977, Adhesion among neural cells of the chick embryo. II. Purification and characterization of a cell adhesion molecule from neural retina.Journal of Biological Chemistry,252, 6841–6845.

    Google Scholar 

  32. Vollmers, H. P., and Birchmeier, W., 1983, Monoclonal antibodies inhibit the adhesion of mouse B16 melanoma cellsin vitro and block lung metastasisin vivo.Proceedings of the National Academy of Sciences U.S.A.,80, 3729–3733.

    Google Scholar 

  33. Wike, E. L., 1971,Data analysis (Chicago, Aldine Atherton).

    Google Scholar 

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Authors and Affiliations

  1. Department of Molecular and Cellular Biology, National Jewish Hospital and Research Center, 80206, Denver, Colorado, USA

    Edward J. McGuire, James J. Mascali & Sharon R. Grady

  2. Department of Tumor Biology, The University of Texas-M.D. Anderson Hospital and Tumor Institute, 77030, Houston, Texas, U.S.A.

    Garth L. Nicolson

Authors
  1. Edward J. McGuire
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  2. James J. Mascali
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  3. Sharon R. Grady
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  4. Garth L. Nicolson
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Address correspondence to this author in Houston.

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McGuire, E.J., Mascali, J.J., Grady, S.R. et al. Involvement of cell-cell adhesion molecules in liver colonization by metastatic murine lymphoma/lymphosarcoma variants. Clin Exp Metast 2, 213–222 (1984). https://doi.org/10.1007/BF00132928

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