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Intermediate filament expression in prostate cancer

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Summary

The human prostate is composed of a series of tubular-alveolar glands and their ducts surrounded by a fibromuscular stroma. The parenchymal glands secrete the seminal fluid and are anatomically arranged into the central, peripheral, and transitional zones. In this chapter the pattern of intermediate filament expression by the various epithelial components of the ducts, tubuloalveolar glands, and stroma are described. The changes which occur during malignant transformation from normal glands to prostatic intraepithelial neoplasia and subsequent invasive carcinoma are presented. The usefulness of cytokeratin markers in the diagnosis of prostate carcinoma is also discussed.

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References

  1. Ishikawa H, Bischoff R, Holtzer H: Mitosis and intermediate-sized filaments in developing skeletal muscle. J Cell Biol 38: 538–555, 1968

    Google Scholar 

  2. Kurki P, Linder E, Virtanen I, Stenman S: Human smooth muscle autoantibodies reacting with intermediate (100⫗A) filaments. Nature (London) 268: 240–241, 1977

    Google Scholar 

  3. Gordon WE, Bushnell A, Burridge K: Characterization of the intermediate (10 nm) filaments of cultured cells using an autoimmune rabbit antiserum. Cell 13: 249–261 1978

    Google Scholar 

  4. Franke WW, Schmid E, Osborn M, Weber K: Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci USA 75: 5034–5038, 1978

    Google Scholar 

  5. Lazarides E: Intermediate filaments: A chemically heterogenous, developmentally regulated class of proteins. Ann Rev Biochem 51: 219–250, 1982

    Google Scholar 

  6. Franke WW: Nuclear lamins and cytoplasmic intermediate filament proteins: A growing multigene family. Cell 48: 3–4, 1987

    Google Scholar 

  7. Lendahl U, Zimmerman LB, McKay RDG: CNS stem cells express a new class of intermediate filament protein. Cell 60: 585–595, 1990

    Google Scholar 

  8. Portier M-M, DeNechaud B, Gross F: Peripherin, a new member of the intermediate filament protein family. Dev Neurosci 6: 335–344, 1984

    Google Scholar 

  9. Fliegner KH, Ching GY, Liem RK: The predicted amino acid sequence of alpha-internexin is that of a novel neuronal intermediate filament protein. EMBO J 9 749–755, 1990

    Google Scholar 

  10. Franke WW, Schmid E, Schiller DL Differentiation-related patterns of expression of proteins of intermediate-sized filaments in tissues and cultured cells. Cold Spring Harbor Symp Quant Biol 46: 431–453, 1982

    Google Scholar 

  11. Osborn M, Weber K: Biology of disease. Tumor diagnosis by intermediate filament typing: A novel tool for surgical pathology. Lab Invest 48: 372–394 1983

    Google Scholar 

  12. Steinert PM, North AC, Parry DAD: Structural features of keratin intermediate filaments [Review]. J Invest Dermatol 103 (Suppl): 195–245, 1994

    Google Scholar 

  13. Quax W, Egberts WV, Hendricks W, Quax-Jeuken Y, Bloemendal H: The structure of the vimentin gene. Cell 35: 215–223, 1983

    Google Scholar 

  14. Quax W, Khan PM, Quax-Jeuken Y, Bloemendal H: The human desmin and vimentin genes are located on different chromosomes. Gene 38: 189–196, 1985

    Google Scholar 

  15. Quax-Jeuken Y, Quax WJ, Bloemendal H: Primary and secondary structure of hamster vimentin predicted from the nucleotide sequence. Proc Natl Acad Sci USA 80: 3548–3552, 1983

    Google Scholar 

  16. Capetanaki YG, Ngai J, Lazarides E: Characterization and regulation in the expression of a gene coding for the intermediate filament protein desmin. Proc Natl Acad Sci USA 81: 6909–6013, 1984

    Google Scholar 

  17. Quax WJ, van den Heuvel R, Egberts WV, Quax-Jeuken Y, Bloemendal H: Intermediate filament cDNAs from BHK-21 cells: Demonstration of distinct genes for desmin and vimentin in all vertebrates classes. Proc Natl Acad Sci USA 81: 5970–5974, 1984

    Google Scholar 

  18. Quax W, van den Broek L, Egberts WV, Ramaekers F, Bloemendal H: Characterization of the hamster desmin gene: Expression and formation of desmin filaments in nonmuscle cells after gene transfer. Cell 43: 327–338, 1985

    Google Scholar 

  19. Balcarek J, Cowan NJ: Structure of the mouse glial fibrillary acidic protein gene: Implications for the evolution of the intermediate filament multigene family. Nucl Acids Res 13: 5527–5543, 1985

    Google Scholar 

  20. Lewis SA, Balcarek JM, Krek V, Shelanski ML, Cowan NJ: Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: Structural conservation of intermediate filaments. Proc Natl Acad Sci USA 81: 2743–2746, 1984

    Google Scholar 

  21. Julien JP, Ramachandran K, Grosveld F: Cloning of a cDNA encoding the smallest neurofilament protein from the rat. Biochim Biophys Acta 825: 398–404, 1985

    Google Scholar 

  22. Lewis SA, Cowan NJ: Genetics, evolution, and expression of the 68,000 MW neurofilament protein: Isolation of a cloned cDNA probe. J Cell Biol 100: 843–850, 1985

    Google Scholar 

  23. Bader BL, Magin TM, Hatzfeld M, Franke WW: Amino acid sequence and gene organization of cytokeratin No. 19, an exceptional tail-less intermediate filament protein. EMBO J 5: 1865–1875, 1986

    Google Scholar 

  24. Glass C, Kim KH, Fuchs E: Sequence and expression of a human type II mesothelial keratin. J Cell Biol 101: 2366–2373, 1985

    Google Scholar 

  25. Hanukoglu I, Fuchs E: The cDNA sequence of a human epidermal keratin: Divergence of sequence but conservation of structure among intermediate filament proteins. Cell 31: 243–252, 1982

    Google Scholar 

  26. Hanukoglu I, Fuchs E: The cDNA sequence of a type II cytoskeletal keratin reveals constant and variable structural domains among keratins. Cell 393 915–924, 1983

    Google Scholar 

  27. Hoffman W, Franz JK: Amino acid sequence of the carboxyterminal part of an acidic type I cytokeratin of molecular weight 51,000 from theXenopus laevis epidermis as predicted from the cDNA sequence. EMBO J 3: 1301–1306, 1984

    Google Scholar 

  28. Hoffman W, Franz JK, Franke WW: Amino acid sequence microbeterogeneities of basic (type II) cytokeratins ofXenopus laevis epidermis and evolutionary conservativity of helical and non-helical domains. J Mol Biol 184: 713–724, 1985

    Google Scholar 

  29. Johnson LD, Idler WW, Ahou WM, Roop DR, Steinert PM: Structure of a gene for the human epidermal 67-kDa keratin. Proc Natl Acad Sci USA 82: 1896–1900, 1985

    Google Scholar 

  30. Jorcano JL, Franz JK, Franke WW: Amino acid sequence diversity between bovine epidermal cytokeratin polypeptides of the basic (type II) subfamily I. Differentiation 28: 155–163, 1984

    Google Scholar 

  31. Jorcano JL, Rieger M, Franz JK, Schiller DL, Moll R, Franke WW: Identification of two types of keratin polypeptides within the acidic cytokeratin subfamily. J Mol Biol 179: 257–281, 1984

    Google Scholar 

  32. Knapp B, Rentrop M, Schweizer J, Winter H: Nonepithelial members of the keratin multigene family: cDNA sequences andin situ localization of the mRNAs. Nucl Acids Res 14: 751–763, 1986

    Google Scholar 

  33. Lehnert ME, Jorcano JL, Zentgraf H, Blessing M, Franz JK, Franke WW: Characterization of bovine keratin genes: Similarities of exon patterns in genes coding for different keratins. EMBO J 32: 3279–3287, 1984

    Google Scholar 

  34. Magin TM, Jorcano JL, Franke WW: Cytokeratin expression in simple epithelia. II. cDNA cloning and sequence characteristic of bovine cytokeratin (No. 8). Differentiation 30: 254–264, 1986

    Google Scholar 

  35. Marchuk D, McCrohon S, Fuchs E: Complete sequence of gene encoding a human type I keratin: Sequences homologous to enhance elements in the regulatory region of the gene. Proc Natl Acad Sci USA 82: 1609–1613, 1985

    Google Scholar 

  36. Ray Chaudhury A, Marshuk D, Lindhurst M, Fuchs E: Three tightly linked genes encoding human type I keratins: Conservation of sequence in the 5′-upstream regions of the coexpressed keratin genes. Mol Cell Biol 6: 539–548, 1986

    Google Scholar 

  37. Romano V, Hatzfeld M, Magin TM: Cytokeratin expression in simple epithelia. 1. Identification of mRNA coding for human cytokeratin No. 18 by a cDNA clone. Differentiation 30: 244–253, 1986

    Google Scholar 

  38. Singer PA, Trevor K, Oshima RG: Molecular cloning and characterization of the endo B cytokeratin expressed in preimplantation mouse embryos. J Biol Chem 261: 538–547, 2986, 1986

    Google Scholar 

  39. Steinert P, Rice R, Roop D, Trus B, Steven A: Complete amino acid sequence of a mouse epidermal keratin subunit and implications for the structure of intermediate filaments. Nature 302: 794–800, 1983

    Google Scholar 

  40. Steinert P, Parry D, Racoosin E: The complete cDNA and deduced amino acid sequence of a type II mouse epidermal keratin of 60,000 kDa: Analysis of sequence differences between type I and type II keratins. Proc Natl Acad Sci USA 81: 5709–5713, 1984

    Google Scholar 

  41. Tyner A, Eichman M, Fuchs E: The sequence of a type II keratin gene expressed in human skin: Conservation of structure among all intermediate filament genes. Proc Natl Acad Sci USA 82: 4683–4687, 1985

    Google Scholar 

  42. Fisher DZ, Chaudbury M, Blobel G: cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filaments proteins. Proc Natl Acad Sci USA 83: 6450–6454, 1986

    Google Scholar 

  43. McKeon FD, Kirschner MW, Caput D: Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins. Nature 319: 463–468, 1986

    Google Scholar 

  44. Weber K, Geisler N: Intermediate filaments: Structural conservation and divergence. Ann NY Acad Sci 455: 126–143, 1985

    Google Scholar 

  45. Steinert PM, Roop DR: Molecular and cellular biology of intermediate filaments. Ann Rev Biochem 57: 593–625, 1988

    Google Scholar 

  46. Roop DR, Cheg CK, Titterington L: Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259: 8037–8040, 1984

    Google Scholar 

  47. Weber K, Shaw G, Osborn M, Debus E, Geisler M: Neurofilament, a subclass of intermediate filaments: Structure and expression. Cold Spring Habor Symp Quant Biol 47: 717–729, 1983

    Google Scholar 

  48. Fuchs E, Marchuk D: Type I and type II keratins have evolved from lower eukaryocytes to form the epidermal intermediate filaments in mammalian skin. Proc Natl Acad Sci USA 80: 5857–5861, 1983

    Google Scholar 

  49. Kim KH, Rheinwald JF, Fuchs EV: Tissue specificity of epithelial keratins: Differential expression of mRNAs for two multigene families. Mol Cell Biol 3: 495–502, 1983

    Google Scholar 

  50. Lewis SA, Cowan NJ: Anomalous placement of introns is a member of the intermediate filament multigene family: An evolutionary conundrum. Mol Cell Biol 6: 1529–1534, 1986

    Google Scholar 

  51. Schiller DL, Franke WW, Geiger B: A subfamily of relatively large and basic cytokeratin polypeptides as defined by peptide mapping is represented by one or several polypeptides in epithelial cells. EMBO J 1: 761–769, 1982

    Google Scholar 

  52. Geisler N, Kaufmann E, Fischer S, Plessman U, Weber K: Neurofilament architecture combines structural principles of intermediate filaments with carboxy-terminal extensions increasing in size between triplet protein. EMBO J 2: 1295–1302, 1983

    Google Scholar 

  53. Geisler M, Kaufmann E, Weber K: Protein chemical characterization of three structurally distinct domains along the protofilament unit of desmin 10 nm filaments. Cell 30: 277–286, 1982

    Google Scholar 

  54. Eicher R, Bonitz P, Sun TT: Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol 98: 1388–1396, 1984

    Google Scholar 

  55. Sun TT, Eicher R, Schermer A, Cooper D, Nelson WG, Weiss RA: Classification, expression, and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. In: Levine AJ, Van de Wonde GF, Topp WC, Watson JD (eds) The Transformed Phenotype: Cancer Cells. Cold Spring Harbor Laboratory, NY, 169–176, 1984

    Google Scholar 

  56. Sun TT, Scheffer CG, Tseng A: Monoclonal antibody studies of mammalian epithelial keratin. A review. Ann NY Acad Sci 455: 307–329, 1984

    Google Scholar 

  57. Goldman R, Goldman A, Green K, Jones J, Lieska M, Yang H-Y: Intermediate filaments: Possible functions as cytoskeletal connecting links between the nucleus and the cell surface. NY Acad Sci 455: 1–17, 1985

    Google Scholar 

  58. Nagle RB, Bocker W, Davis J, Heid HW, Kaufman M, Lucas DO, Jarasch ED: Characterization of breast carcinoma by two monoclonal antibodies distinguishing myoepithelial from luminal epithelial cells. J Histochem Cytochem 34: 869–881, 1986

    Google Scholar 

  59. Pruss RM, Mirsk R, Ruff MC, Thorpe R, Dowding AJ, Anderson BH: All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody. Cell 27: 419–428, 1981

    Google Scholar 

  60. Parry DAD, Fraer RBD: Intermediate filament structure. 1. Analysis of IF protein sequence data. Int J Biol Macromol 7: 203–213, 1985

    Google Scholar 

  61. Quinlan RA, Cohlberg JA, Schiller DL, Hatzfeld M, Franke WW: Heterotypic tetramer (A2D2) complexes of nonepithelial keratins isolated from cytoskeletons of rat hepatocytes and hepatoma cells. J Mol Biol 78: 365–388, 1984

    Google Scholar 

  62. Hatzfeld M, Franke WW: Pair formation and promiscuity of cytokeratins: Formationin vitro of heterotypic complexes and intermediate-sized filaments by homologous and heterologous recombinations of purified polypeptides. J Cell Biol 101: 1826–1841, 1985

    Google Scholar 

  63. Coulombe PA, Fuchs E: Elucidating the early stages of keratin filament assembly. J Cell Biol 111: 153–169, 1990

    Google Scholar 

  64. Steven AC, Hainfeld JF, Trus BL, Wall JS, Steinert PM: The distribution of mass in heteropolymer intermediate filaments assembledin vitro: STEM analysis of vimentin/desmin and bovine epidermal keratin. J Biol Chem 258: 8323–8329, 1983

    Google Scholar 

  65. Steven AC, Hainfeld JF, Trus BL, Wall JS, Steinert PM: Epidermal keratin filaments assembledin vitro have massesper-unit-length that scale according to average subunit mass: Structural basis for homologous packing of subunits in intermediate filaments. J Cell Biol 97: 1939–1044, 1983

    Google Scholar 

  66. Steven AC, Hainfeld JF, Trus BL, Wall JS, Steinert PM: Conformity and diversity in the structures of intermediate filaments. Ann NY Acad Sci 455: 371–380, 1985

    Google Scholar 

  67. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors, and cultured cells. Cell 31: 11–24, 1982

    Google Scholar 

  68. Moll R, Franke WW, Volc-Platzer B, Krepler R: Different keratin polypeptides in epidermis and other epithelia of human skin: A specific cytokeratin of molecular weight 36,000 in epithelia of the pilosebaceous tract and basal cell. J Cell Biol 95: 285–295, 1982

    Google Scholar 

  69. Moll R, Schiller DL, Franke WW: Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J Cell Biol 111: 567–580, 1990

    Google Scholar 

  70. Lynch MH, O'Guin WM, Hardy C, Mak L, Sun T-T: Acidic and basic hair/nail (‘hard’) keratins: Their co-localization in upper cortical and cuticle cells of the human hair follicle and their relationship to ‘soft’ keratins. J Cell Biol 103: 2593–2606, 1986

    Google Scholar 

  71. Heid HW, Moll I, Franke WW: Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation 37: 137–157, 1988

    Google Scholar 

  72. Leask A, Byrne C, Fuchs E: Transcription factor AP2 and its role in epidermal-specific gene expression. Proc Natl Acad Sci USA 88: 7948–7952, 1991

    Google Scholar 

  73. Leask A, Rosenberg R, Vassar R, Fuchs E: Regulation of a human epidermal keratin gene: Sequences and nuclear factors involved in keratinocyte-specific transcription. Genes Dev 4: 1985–1998, 1990

    Google Scholar 

  74. Wu R-L, Galvin S, Wu SK, Xu C, Blumenberg M, Sun T-T: A 300 bp 5′-upstream sequence of a differentiation-dependent rabbit K3 keratin gene can serve as a keratinocyte-specific promoter. J Cell Sci 105: 303–316, 1993

    Google Scholar 

  75. Byrne C, Fuchs E: Probing keratinocyte and differentiation specificity of the human K5 promoterin vitro and in transgenic mice. Mol Cell Biol 13: 3176–3190, 1993

    Google Scholar 

  76. McNeal JE: The prostate gland: Morphology and pathology. Monographs in Urology 3: 33, 1983

    Google Scholar 

  77. Nagle RB, Brawer MK, Kittelson J, Clark V: Phenotypic relationships of prostatic intraepithelial neoplasia to invasive prostatic carcinoma. Am J Pathol 138: 119–128, 1991

    Google Scholar 

  78. Verhagen Anja PM, Aalders TW, Ramaekers FCS, Debruyne FMJ, Schalken JA: Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration. The Prostate 13: 25–38, 1988

    Google Scholar 

  79. Kuwahra T, Nagayama T: Distribution of keratin protein in normal prostate and prostatic tumors. Acta Pathol (Japan) 37: 339–342, 1987

    Google Scholar 

  80. Wernert N, Seitz G, Achtstatter T: Immunohistochemical investigation of different cytokeratins and vimentin in the prostate from the fetal period up to adulthood and in prostate cardinoma. Pathol Res Pract 182: 617–626, 1987

    Google Scholar 

  81. Sherwood ER, Berg LA, Mitchell NJ, McNeal JE, Kozlowski JM, Lee C: Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. J Urol 143: 167–171, 1990

    Google Scholar 

  82. Oertel H: Involutionary changes in prostate and female breast in relationship to cancer development. Can Med Assoc J 16: 237–241, 1925

    Google Scholar 

  83. McNeal JE, Bostwick DG: Intraductal dysplasia: A premalignant lesion of the prostate. Hum Pathol 17: 64–71, 1986

    Google Scholar 

  84. Bostwick DG, Amin MB, Dundore P, Marsh W, Schultz DS: Architectural patterns of high-grade prostatic intraepithelial neoplasia. Hum Pathol 24: 298–310, 1993

    Google Scholar 

  85. Petein M, Michel P, Van Velthoven R, Pasteels J-L, Brawer MK, Davis JR, Nagle RB, Kiss R: Morphonuclear relationship between prostatic intraepithelial neoplasia and cancers as assessed by digital cell image analysis. Am J Clin Pathol 96: 628–634, 1991

    Google Scholar 

  86. Brawer MK, Peehl DM, Stamey TA, Bostwick DG: Keratin immunoreactivity in the benign and neoplastic human prostate. Cancer Res 45: 3663–3667, 1985

    Google Scholar 

  87. Nagle RB, Ahmann FR, McDaniel KM, Paquin ML, Clark VA, Cleniker A: Cytokeratin characterization of human prostatic carcinoma and its derived cell lines. Cancer Res 47: 281–286, 1987

    Google Scholar 

  88. Hedrick L, Epstein JI: Use of keratin 903 as an adjunct in the diagnosis of prostate carcinoma. Am J Surg Pathol 13: 389–396, 1989

    Google Scholar 

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  1. Department of Pathology, University of Arizona Health Sciences Center, 85724-5043, Tucson, AZ, USA

    Ray B. Nagle

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Nagle, R.B. Intermediate filament expression in prostate cancer. Cancer Metast Rev 15, 473–482 (1996). https://doi.org/10.1007/BF00054013

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