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Deferoxamine Reduces Cavity Size in the Brain After Intracerebral Hemorrhage in Aged Rats

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Intracerebral Hemorrhage Research

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 111))

Abstract

This study investigated whether deferoxamine (DFX), an iron chelator, reduces cavity size after ICH in aged rats. Aged male Fischer rats (18 months old) had an intracaudate injection of 100 μL autologous blood and were treated with DFX or vehicle. Rats were euthanized at day 56 and brains were perfused for histology and immunohistochemistry. Hematoxylin and eosin staining was used to examine hematoma cavity presence and size. Immunohistochemistry was performed to measure the number of cells positive for ferritin, heme oxygenase-1 (HO-1), glial fibrillary acidic protein (GFAP) and OX-6. Neurological deficits were also examined. In aged rats with ICH, a cavity formed in the caudate in 7 out of 12 vehicle-treated rats and 1 out of 9 DFX-treated rats. DFX treatment significantly reduced the size of the ICH-induced cavity (p < 0.05) as well as neurological deficits (p < 0.05). DFX also reduced the number of ferritin (p < 0.05) and HO-1 (p < 0.01) positive cells in the ipsilateral basal ganglia. However, DFX had no effect on brain GFAP and OX-6 immunoreactivity 2 months after ICH.

In conclusion, DFX reduces cavity size, neurological deficits, and immunoreactivity for ferritin and HO-1 after ICH in aged rats, supporting the suggestion that DFX may reduce brain injury in ICH patients.

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Acknowledgements

This study was supported by grants NS-017760, NS-039866 and NS-057539 from the National Institutes of Health (NIH) and 0755717Z and 0840016N from the American Heart Association (AHA). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH and AHA.

Conflict of interest statement We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA

    Tetsuhiro Hatakeyama, Masanobu Okauchi, Ya Hua & Richard F. Keep

  2. Department of Neurosurgery, Kagawa University, Takamatsu, Kagawa, Japan

    Tetsuhiro Hatakeyama & Masanobu Okauchi

  3. Department of Neurosurgery, University of Michigan, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA

    Guohua Xi

Authors
  1. Tetsuhiro Hatakeyama
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  2. Masanobu Okauchi
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  3. Ya Hua
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  4. Richard F. Keep
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  5. Guohua Xi
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Corresponding author

Correspondence to Guohua Xi .

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Editors and Affiliations

  1. , Neurosurgery, Physiology, Loma Linda University, Campus Street 11041, Loma Linda, 92530, USA

    John Zhang

  2. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    Austin Colohan

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Hatakeyama, T., Okauchi, M., Hua, Y., Keep, R.F., Xi, G. (2011). Deferoxamine Reduces Cavity Size in the Brain After Intracerebral Hemorrhage in Aged Rats. In: Zhang, J., Colohan, A. (eds) Intracerebral Hemorrhage Research. Acta Neurochirurgica Supplementum, vol 111. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0693-8_31

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  • DOI: https://doi.org/10.1007/978-3-7091-0693-8_31

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-0692-1

  • Online ISBN: 978-3-7091-0693-8

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