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Isolation of Stem Cells from Human Adipose Tissue: Technique, Problems, and Pearls

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Adipose Stem Cells and Regenerative Medicine

Abstract

The comprehension of the basic concepts of stem cells is easily explained (their isolation, animal models, difficulties, and an outlook for their use, along with points that must be considered in an actual reality). Cloning, homing, and induction of differentiation are pearls that are approached, and certainly may help plastic surgeons understand this. What is real and what is still not real regarding these fantastic sources of inspiration and cure expectancy are considered.

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References

  1. Almeida KA, Campa A, Alonso-Vale MIC et al (2008) Stromal vascular fraction from fat tissue: obtaining stem cells and their yield according to the topography of the donor areas: previous note. Cir Plást Iberolatinoam 34(1):71–77

    Article  Google Scholar 

  2. Barry FP, Murphy JM (2004) Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol 36:568–584

    Article  PubMed  CAS  Google Scholar 

  3. Gimble JM, Guilak F (2003) Adipose-derived adult stem cells: isolation, characterization and differentiation potential. Cytotherapy 5:362–369

    Article  PubMed  Google Scholar 

  4. Iacovella T, Bentini R, Filippin FB et al (2009) Proliferation of adipose-derived mesenchymal stem cells in polylactic acid-derived biomembranes. Poster session presented at the 24th Annual Conference of the Federation of Brazilian Societies for Experimental Biology (FeSBE), Águas de Lindóia, Brazil, 19–22 Aug 2009

    Google Scholar 

  5. Lu LL, Liu YJ, Yang SG et al (2006) Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials. Haematologica 91:1017–1026

    PubMed  CAS  Google Scholar 

  6. Mitchell JB, McIntosh K, Zvonic S et al (2006) Immuno­phenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers. Stem Cells 24:376–385

    Article  PubMed  Google Scholar 

  7. Ogawa R, Mizuno H, Watanabe H et al (2004) Osteogenic and chondrogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice. Biochem Biophys Res Commun 313:871–877

    Article  PubMed  CAS  Google Scholar 

  8. Rigotti G, Marchi A, Galiè M et al (2007) Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose-derived adult stem cells. Plast Reconstr Surg 119(5):1409–1422

    Article  PubMed  CAS  Google Scholar 

  9. Rodbell M (1964) Metabolism of isolated fat cells. Effects of hormones on glucose metabolism and lipolysis. J Biol Chem 239:357–380

    Google Scholar 

  10. Stocchero IN, Stocchero GF, Stocchero G et al (2006) Liposuctionable fat: a hypothetic model. Plast Reconstr Surg 117(1):337–338

    Article  PubMed  CAS  Google Scholar 

  11. Stocchero IN, Stocchero GHF, Stocchero GF et al (2009) Lamellar fatgrafting: a promising treatment with stromal vascular fraction in recurrent vulvovaginitis. Cir Plást Iberolatinoam 35(3):179–186

    Article  Google Scholar 

  12. Torres FC, Rodrigues CJ, Stocchero IN et al (2007) Stem cells from the fat tissue of rabbits: an easy-to-find experimental source. Aesth Plast Surg 31(5):574–578

    Article  Google Scholar 

  13. Yoshimura K (2008) Clinical use of adipose-derived stem cells for breast volume enhancement. ISAPS News 2(3):6

    Google Scholar 

  14. Zuk PA, Zhu M, Mizuno H et al (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228

    Article  PubMed  CAS  Google Scholar 

  15. Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Ithamar Nogueira Stocchero .

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© 2011 Springer-Verlag Berlin Heidelberg

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Stocchero, I.N., Stocchero, G.F. (2011). Isolation of Stem Cells from Human Adipose Tissue: Technique, Problems, and Pearls. In: Illouz, YG., Sterodimas, A. (eds) Adipose Stem Cells and Regenerative Medicine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20012-0_2

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  • DOI: https://doi.org/10.1007/978-3-642-20012-0_2

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-20011-3

  • Online ISBN: 978-3-642-20012-0

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